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公开(公告)号：WO2020190998A1
公开(公告)日：2020-09-24
申请号：PCT/US2020/023260
申请日：2020-03-18
Applicant: BAYER CROPSCIENCE LP , SPOGEN BIOTECH INC.
Inventor： AUGUSTIN, Jörg , CURTIS, Damian , HENRY, Elizabeth M. , HOTTON, Sara K. , LAMSA, Anne , MANAVALAN, Lakshmi Praba , THOMAS, Varghese P. , THOMPSON, Brian
Abstract: The present invention relates to a fusion protein having a targeting sequence, exosporium protein, or exosporium protein fragment that targets the fusion protein to the exosporium of a recombinant Bacillus cereus family member and a pectinase enzyme, wherein the pectinase is a pectate lyase from Bacillus spp. having any of SEQ ID NOs: 213-217 and 222-226 or a polygalaturonase from Aspergillus niger or certain Bacillus species that can have any of SEQ ID NOs: 210-212, 218-221, and 227. The present invention also provides a recombinant Bacillus cereus family member that expresses such fusion protein and exosporium fragments derived from such recombinant Bacillus cereus family member. Methods of using such recombinant Bacillus cereus family members or exosporium fragments derived therefrom for plant growth promotion are also provided.
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公开(公告)号：WO2019221988A8
公开(公告)日：2019-11-21
申请号：PCT/US2019/031264
申请日：2019-05-08
Applicant: BAYER CROPSCIENCE LP
Inventor： COLLINS, Jennifer Anne , SANCHEZ, Jesus F. , WALLER, James L. , TRAAG, Bjorn A. , HOTTON, Sara K. , TEGELER, Tony J.
IPC: C07K14/195 , A01N63/04 , C12N1/20 , C12R1/01 , A01N63/02
Abstract: The present invention provides a composition comprising a biologically pure culture of a Paenibacillus sp. strain comprising a mutant DegU lacking a functional receiver domain or a functional DNA binding domain and/or a mutant DegS lacking a functional single binding domain or a functional ATPase domain with decreased viscosity in a liquid culture. Also provided is a method of identifying a Paenibacillus sp. mutant derivative strain with decreased viscosity in a liquid culture compared to a Paenibacillus sp. parental strain with a visual screen for mutant isolates with a non-mucoid morphology.
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公开(公告)号：WO2019221988A1
公开(公告)日：2019-11-21
申请号：PCT/US2019/031264
申请日：2019-05-08
Applicant: BAYER CROPSCIENCE LP
Inventor： COLLINS, Jennifer Anne , SANCHEZ, Jesus F. , WALLER, James L. , TRAAG, Bjorn A. , HOTTON, Sara K. , TEGELER, Tony J.
IPC: C07K14/195 , A01N63/04 , C12N1/20 , C12R1/01 , A01N63/02
Abstract: The present invention provides a composition comprising a biologically pure culture of a Paenibacillus sp. strain comprising a mutant DegU lacking a functional receiver domain or a functional DNA binding domain and/or a mutant DegS lacking a functional single binding domain or a functional ATPase domain with decreased viscosity in a liquid culture. Also provided is a method of identifying a Paenibacillus sp. mutant derivative strain with decreased viscosity in a liquid culture compared to a Paenibacillus sp. parental strain with a visual screen for mutant isolates with a non-mucoid morphology.
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