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    COMPOSITIONS COMPRISING RECOMBINANT BACILLUS CELLS AND ANOTHER BIOLOGICAL CONTROL AGENT
    1.
    发明申请
    COMPOSITIONS COMPRISING RECOMBINANT BACILLUS CELLS AND ANOTHER BIOLOGICAL CONTROL AGENT 审中-公开
    包含重组BACILLUS细胞和另一种生物控制剂的组合物

    公开(公告)号：WO2016044548A1

    公开(公告)日：2016-03-24

    申请号：PCT/US2015/050621

    申请日：2015-09-17

    Applicant: BAYER CROPSCIENCE LP ,  SPOGEN BIOTECH INC.

    Inventor： CURTIS, Damian ,  THOMPSON, Brian

    IPC: A01N63/00 ,  A01N63/04 ,  A01P21/00

    CPC classification number: C07K14/325 ,  A01N25/00 ,  A01N63/00 ,  A61K38/00 ,  C12N15/00 ,  C12N15/63 ,  C12N15/82 ,  C12N15/866 ,  A01N63/04

    Abstract: The present invention relates to a composition comprising a) recombinant exosporium-producing Bacillus cells that express a fusion protein comprising: (i) at least one plant growth stimulating protein or peptide and (ii) a targeting sequence that localizes the fusion protein to the exosporium of the Bacillus cells; and b) at least one further biological control agent selected from particular microorganisms disclosed herein and/or a mutant of a specific strain of a microorganism disclosed herein having all identifying characteristics of the respective strain, and/or at least one metabolite produced by the respective strain, and/or at least one metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and/or phytopathogens in a synergistically effective amount. Furthermore, the present invention relates to the use of this composition as well as a method for enhancing plant growth, promoting plant health, and/or reducing overall damage of plants and plant parts.

    Abstract translation: 本发明涉及一种组合物，其包含：a）表达融合蛋白的重组芽孢杆菌产生芽孢杆菌细胞，所述融合蛋白包含：（i）至少一种植物生长刺激蛋白或肽 和（ii）将融合蛋白定位于芽孢杆菌属细胞的外生孢子的导向序列; 和b）选自本文公开的特定微生物的至少一种其他生物防治剂和/或本文公开的微生物特定菌株的突变体，其具有各自菌株的全部鉴定特征，和/或由各自产生的至少一种代谢产物 和/或由相应菌株产生的至少一种代谢物，其以协同有效量表现出对昆虫，螨，线虫和/或植物病原体的活性。 此外，本发明涉及该组合物的用途以及增强植物生长，促进植物健康和/或减少植物和植物部分的整体损害的方法。

    PLANT GROWTH-PROMOTING BACTERIA AND METHODS OF USE
    5.
    发明申请
    PLANT GROWTH-PROMOTING BACTERIA AND METHODS OF USE 审中-公开
    植物生长促进细菌和使用方法

    公开(公告)号：WO2014145883A1

    公开(公告)日：2014-09-18

    申请号：PCT/US2014/030726

    申请日：2014-03-17

    Applicant: SPOGEN BIOTECH INC.

    Inventor： THOMPSON, Brian ,  THOMPSON, Katie ,  ANGLE, Brittany

    IPC: C12N1/20 ,  A01N63/02

    CPC classification number: A01N63/00 ,  A01H3/00 ,  A01N63/02 ,  C12R1/01 ,  C12R1/05 ,  C12R1/07 ,  C12R1/075 ,  C12R1/085 ,  C12R1/125 ,  A01N25/00

    Abstract: The present invention generally relates to bacteriologically pure bacterial cultures of novel strains of plant growth-promoting bacteria, and inoculums comprising the same. The invention is also directed to plant seeds coated with the inoculums, kits comprising the inoculums and methods for stimulating plant growth by applying the biologically pure bacterial culture or the inoculum to a plant, plant seed, or plant growth medium.

    Abstract translation: 本发明一般涉及植物生长促进细菌新菌株的细菌学纯细菌培养物和包含其的接种物。 本发明还涉及用接种物包被的植物种子，包含接种物的试剂盒和通过将生物学纯细菌培养物或接种物施用于植物，植物种子或植物生长培养基来刺激植物生长的方法。

    FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS
    6.
    发明申请
    FUSION PROTEINS AND METHODS FOR STIMULATING PLANT GROWTH, PROTECTING PLANTS, AND IMMOBILIZING BACILLUS SPORES ON PLANTS 审中-公开
    融合蛋白和刺激植物生长的方法，保护植物和植物上的苗木运动

    公开(公告)号：WO2014145964A1

    公开(公告)日：2014-09-18

    申请号：PCT/US2014/030824

    申请日：2014-03-17

    Applicant: SPOGEN BIOTECH INC.

    Inventor： THOMPSON, Brian ,  THOMPSON, Katie

    IPC: C07K19/00 ,  C12N15/62 ,  C12N1/21

    CPC classification number: C07K14/32 ,  A01N37/46 ,  A01N37/48 ,  A01N63/00 ,  A01N63/02 ,  C07K2319/035 ,  C12N9/0095 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/48 ,  C12Y118/06001 ,  C12Y301/01003 ,  C12Y301/03008 ,  C12Y302/01004 ,  C12Y304/00

    Abstract: The present invention is generally directed to fusion proteins containing a targeting sequence that targets the fusion protein to the exosporium of a Bacillus cereus family member. The invention also relates to recombinant Bacillus cereus family members expressing such fusion proteins and formulations containing the recombinant Bacillus cereus family members expressing the fusion proteins. Methods for stimulating plant growth, for protecting plants from pathogens, and for enhancing stress resistance in a plant by applying the recombinant Bacillus cereus family members or the formulations to plants or a plant growth medium are also described. The invention also relates to methods for immobilizing spores of a recombinant Bacillus cereus family member expressing a fusion protein on plants.

    Abstract translation: 本发明一般涉及含有将融合蛋白靶向蜡状芽孢杆菌家族成员的外植体的靶向序列的融合蛋白。 本发明还涉及表达这种融合蛋白的重组蜡状芽孢杆菌家族成员和含有表达融合蛋白的重组蜡状芽孢杆菌家族成员的制剂。 还描述了通过将重组蜡状芽孢杆菌家族成员或制剂施用于植物或植物生长培养基来刺激植物生长，保护植物免受病原体和增强植物胁迫抗性的方法。 本发明还涉及将表达融合蛋白的重组蜡状芽孢杆菌家族成员的孢子固定在植物上的方法。

    FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA
    7.
    发明申请
    FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA 审中-公开

    公开(公告)号：WO2016044655A3

    公开(公告)日：2016-03-24

    申请号：PCT/US2015/050795

    申请日：2015-09-17

    Applicant: SPOGEN BIOTECH INC.

    Inventor： THOMPSON, Brian ,  SIEGEL, Ashley

    IPC: C07K14/32

    Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

    BIOACTIVE POLYPEPTIDES FOR IMPROVEMENTS IN PLANT PROTECTION, GROWTH AND PRODUCTIVITY
    9.
    发明申请
    BIOACTIVE POLYPEPTIDES FOR IMPROVEMENTS IN PLANT PROTECTION, GROWTH AND PRODUCTIVITY 审中-公开

    公开(公告)号：WO2019018768A1

    公开(公告)日：2019-01-24

    申请号：PCT/US2018/043092

    申请日：2018-07-20

    Applicant: SPOGEN BIOTECH INC.

    Inventor： THOMPSON, Brian ,  LESLIE, Michelle

    IPC: A61K38/00 ,  A61K38/16 ,  C07K7/00 ,  C07K7/06 ,  C07K14/00 ,  C07K14/195

    Abstract: Bioactive priming polypeptides are provided that are useful when applied to plants in agricultural formulations. Methods of using the formulations containing the bioactive priming polypeptides are also provided which are applied exogenously to the surface of a plant or a plant cell membrane or endogenously to the interior of a plant or to a plant cell. The bioactive priming polypeptides when applied to a plant, a plant part, or a plant growth medium or a rhizosphere in an area surrounding the plant or the plant part increase growth, yield, health, longevity, productivity, and/or vigor of a plant or a plant part and/or decrease abiotic stress in the plant or the plant part and/or protect the plant or the plant part from disease, insects and/or nematodes, and/or increase the innate immune response of the plant or the plant part and/or change plant architecture.

    FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA
    10.
    发明申请
    FUSION PROTEINS, RECOMBINANT BACTERIA, AND METHODS FOR USING RECOMBINANT BACTERIA 审中-公开
    融合蛋白，重组细菌和使用重组细菌的方法

    公开(公告)号：WO2016044661A1

    公开(公告)日：2016-03-24

    申请号：PCT/US2015/050807

    申请日：2015-09-17

    Applicant: SPOGEN BIOTECH INC.

    Inventor： THOMPSON, Brian ,  SIEGEL, Ashley

    IPC: C07K14/32

    CPC classification number: C07K14/32 ,  A01N37/44 ,  A01N63/02 ,  A23K10/18 ,  A23K20/147 ,  A61K39/00 ,  A61L2/18 ,  C02F3/342 ,  C02F3/348 ,  C02F2101/10 ,  C02F2101/30 ,  C02F2103/002 ,  C02F2103/003 ,  C02F2103/007 ,  C02F2103/06 ,  C02F2103/10 ,  C02F2103/14 ,  C02F2103/16 ,  C02F2103/26 ,  C02F2103/28 ,  C07K2319/01 ,  C07K2319/035 ,  C07K2319/40 ,  C09K8/62 ,  C09K2208/24 ,  C12N1/20 ,  C12N3/00 ,  C12N15/62 ,  C12N15/75 ,  C12P21/02 ,  E21B43/16

    Abstract: Fusion proteins containing a targeting sequence, an exosporium protein, or an exosporium protein fragment that targets the fusion protein to the exosporium of a Bacillus cereus family member are provided. Recombinant Bacillus cereus family members expressing such fusion proteins are also provided. Genetically inactivated Bacillus cereus family members and recombinant Bacillus cereus family members that overexpress exosporium proteins are also provided. Seeds coated with the recombinant Bacillus cereus family members and methods for using the recombinant Bacillus cereus family members (e.g., for stimulating plant growth) are also provided. Various modifiations of the recombinant Bacillus cereus family members that express the fusion proteins are further provided. Fusion proteins comprising a spore coat protein and a protein or peptide of interest, recombinant bacteria that express such fusion proteins, seeds coated with such recombinant bacteria, and methods for using such recombinant bacteria (e.g., for stimulating plant growth) are also provided.

    Abstract translation: 提供了融合蛋白，其含有靶向序列，外孢壁蛋白或靶向融合蛋白至蜡样芽孢杆菌家族成员的外孢壁的外孢壁蛋白片段。 还提供了表达这种融合蛋白的重组蜡状芽孢杆菌家族成员。 还提供了基因失活的蜡状芽孢杆菌家族成员和过表达外孢菌蛋白的重组蜡状芽孢杆菌家族成员。 还提供了用重组蜡状芽孢杆菌家族成员包被的种子和使用重组蜡状芽孢杆菌家族成员（例如，用于刺激植物生长）的方法。 进一步提供了表达融合蛋白的重组蜡状芽孢杆菌家族成员的各种修饰。 还提供了包含孢子外壳蛋白和感兴趣的蛋白质或肽的融合蛋白，表达此类融合蛋白的重组细菌，用这种重组细菌包被的种子，以及使用这种重组细菌的方法（例如用于刺激植物生长）。 / p>

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