公开(公告)号：WO2022213077A1

公开(公告)日：2022-10-06

申请号：PCT/US2022/071426

申请日：2022-03-29

申请人： BERKELEY LIGHTS, INC.

发明人： ELMS, Phillip J. M. ,  NG, Preston Lock ,  CHENG, Jonathan ,  VER HEUL, Nathan J. ,  YEE, Jason King Mckenzie ,  CHIRRA DINAKAR, Hariharasudhan ,  TOBIAS, Renee Lynn ,  SACKMANN, Eric K. ,  ZINK, Wesley Arthur ,  NEIRA-QUINTERO, Hector D.

IPC分类号： C12Q1/02 ,  G01N1/00 ,  G01N33/48 ,  G01N33/53

摘要： Methods for identifying a cell population secreting a biomolecule with desirable attributes are disclosed. The desirable attributes can include, for example, quantity and quality (e.g., minimal aggregation and/or desired configuration). Cell populations identified by the disclosed methods are more likely to successfully scale during production. The methods can include assessing multiple domains/binding sites of a complex biomolecule and/or the formation of aggregates by the biomolecules. Methods of assessing a secretion level for biomolecules having a wide range of molecular weights and method for enhancing loading of cells into chambers of a microfluidic device. The labelling of viable cells and/or cells actively expressing a biomolecule of interest, for example, can permit selection and subsequent analysis of the cells most likely to successfully expand and express the biomolecule of interest, thereby reducing effort, risk, and cost associated with the screening of cells and increase the probability of identifying cell lines that are optimal producers of biomolecules of interest.

公开(公告)号：WO2017117408A1

公开(公告)日：2017-07-06

申请号：PCT/US2016/069249

申请日：2016-12-29

申请人： BERKELEY LIGHTS, INC.

发明人： KURZ, Volker L.S. ,  LIONBERGER, Troy A. ,  SACKMANN, Eric K. ,  SZETO, Kai W. ,  LEBEL, Paul M. ,  BRUHN, Brandon R. ,  BREINLINGER, Keith J. ,  HOBBS, Eric D. ,  MCFARLAND, Andrew W. ,  NEVILL, Tanner J. ,  WANG, Xiaohua

IPC分类号： B81B7/02 ,  G01N35/08

CPC分类号： B01L3/502761 ,  B01L2200/0668 ,  B01L2300/1822 ,  B01L2400/0427 ,  B01L2400/0442 ,  B01L2400/0454 ,  B01L2400/086

摘要： Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.

摘要翻译： 描述了使用光学驱动的气泡，对流和置换流体流动以在微流体装置中提供驱动力的装置和方法。 替代动力模式对于选择性地从微流体装置外壳内的各种位置移开和移动微物体（包括生物细胞）是有用的。

公开(公告)号：WO2022216778A1

公开(公告)日：2022-10-13

申请号：PCT/US2022/023598

申请日：2022-04-06

申请人： BERKELEY LIGHTS, INC.

发明人： GADISH, Or ,  MOBILIA, Kellen C. ,  TAFOYA, Sara ,  SACKMANN, Eric K. ,  MASTROIANNI, Alexander J. ,  SHIEH, Peyton ,  SRIDHAR, Smriti ,  SPHABMIXAY, Pierre ,  CHEUNG, Eva Y. ,  WAWRZYN, Grayson T. ,  LE, Long V. ,  VER HEUL, Nathan J. ,  HU, Bo ,  CHAO, Lily ,  LIN, Ke-Chih ,  KURZ, Volker L.S. ,  ONORATO, Robert M.

IPC分类号： B01L3/00 ,  G01N33/52 ,  G01N33/532

摘要： Metabolic engineering has developed microbial cell factories as sustainable alternatives to chemical synthesis from petroleum feedstocks or harvesting of animals and plants, but current methods can be a costly and labor-intensive commitment. Methods are described herein for microfluidic methods of screening thousands of variant cells in order to reduce time and uncertainty to provide improved strains.

公开(公告)号：WO2022047290A1

公开(公告)日：2022-03-03

申请号：PCT/US2021/048196

申请日：2021-08-30

申请人： BERKELEY LIGHTS, INC.

发明人： INGRAM, Patrick N. ,  CHIEN, Alexander ,  LIN, Ke-Chih ,  GADISH, Or ,  LIONBERGER, Troy A. ,  SACKMANN, Eric K. ,  KURZ, Volker L.S. ,  MASTROIANNI, Alexander J. ,  LOWE, JR., Randall D. ,  HUBBARD, Jonathan Cloud Dragon

IPC分类号： A61K49/00 ,  A61B5/145 ,  A61B5/1455 ,  G01N21/64

摘要： Methods, systems, and kits for determining a level of dissolved oxygen within a microfluidic device are provided. The microfluidic device can be suitable for cell culture. The methods, systems, and kits can further be used to determine a level of oxygen consumption in a population of biological micro-objects. In particular, the methods, systems, and kits of the present disclosure rely on flowing a fluidic medium containing a dye and a supplied partial pressure of oxygen into the microfluidic device for a period of time, wherein fluorescence emitted by the dye changes based on availability of oxygen proximate to the dye; taking a fluorescence image of an area of interest within the chamber; and correlating fluorescence of the fluorescence image of the area of interest to a reference to determine an observed partial pressure of the oxygen in the area of interest.

公开(公告)号：WO2020077274A1

公开(公告)日：2020-04-16

申请号：PCT/US2019/055953

申请日：2019-10-11

申请人： BERKELEY LIGHTS, INC.

发明人： MCEWEN, Jason M. ,  LIONBERGER, Troy A. ,  SACKMANN, Eric K. ,  KURZ, Volker L.S. ,  MOBILIA, Kellen C.

IPC分类号： B01F3/08 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  C12N15/10 ,  C12P21/00

摘要： Systems, methods, and kits therefor, enabling rapid protein evolution are described herein. A system useful in the methods described herein include a DNA synthesis component; a microfluidic system including a microfluidic device having a microfluidic channel and sequestration pens; and a computing component, which is configured to analyze assay results and, based upon the analysis, design improved DNA sequences for iterative protein evolution. The microfluidic system is configured to permit correlation of DNA sequence on a bead to its location within the microfluidic device, permit cell free protein expression of a DNA sequence captured to a bead, and to permit assay of the protein so produced.