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    METHODS FOR PHENOTYPING OF INTACT BONES BY TISSUE CLEARING AND STAINING
    3.
    发明申请
    METHODS FOR PHENOTYPING OF INTACT BONES BY TISSUE CLEARING AND STAINING 审中-公开
    通过组织清除和染色法对真实骨骼进行成像的方法

    公开(公告)号：WO2016073941A1

    公开(公告)日：2016-05-12

    申请号：PCT/US2015/059600

    申请日：2015-11-06

    申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

    发明人： GRADINARU, Viviana ,  YANG, Bin ,  TREWEEK, Jennifer ,  CHAN, Ken

    IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/569 ,  G01N1/30

    CPC分类号： G01N1/30 ,  G01N2001/302

    摘要： In various embodiments, the present application teaches methods and kits for clearing and optionally subsequently visualizing tissue containing bone. In some embodiments, the method includes serially incubating cleared bone in refractive index matching solutions with progressively higher refractive indexes. In some embodiments, the methods teach immunolabeling and/or staining tissue containing bone and optionally visualizing the immunolabeled and/or stained tissue containing bone.

    摘要翻译： 在各种实施方案中，本申请教导了用于清除和任选地随后使包含骨组织可视化的方法和试剂盒。 在一些实施方案中，所述方法包括在折射率匹配溶液中连续培养清除的骨，并且逐渐变高的折射率。 在一些实施方案中，所述方法教导了含有骨的组织的免疫标记和/或染色，并任选地使包含免疫标记和/或染色的组织含骨显色。

    ADENO-ASSOCIATED VIRUS COMPOSITIONS FOR TARGETED GENE THERAPY
    6.
    发明申请
    ADENO-ASSOCIATED VIRUS COMPOSITIONS FOR TARGETED GENE THERAPY 审中-公开

    公开(公告)号：WO2020068990A1

    公开(公告)日：2020-04-02

    申请号：PCT/US2019/052969

    申请日：2019-09-25

    申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

    发明人： GRADINARU, Viviana ,  FLYTZANIS, Nicholas C. ,  GOEDEN, Nicholas S.

    IPC分类号： C12N15/86 ,  C07K7/06 ,  C07K14/015

    摘要： Described herein are compositions and kits comprising recombinant adeno-associated viruses (rAAVs) with tropisms showing increased specificity and efficiency of viral transduction in targeted cell-types, for e.g., the brain, and lung. The rAAV compositions described herein also have tropisms showing decreased specificity and decreased efficiency of viral transduction in an off-target cell type, for e.g., the liver. The rAAV compositions described herein encapsidate a transgene, such a therapeutic nucleic acid. Upon systemic delivery to a subject, the rAAV is capable of increased specificity and increased transduction of the transgene in a target cell-type, as compared to a parental or reference AAV.

    METHODS FOR PHENOTYPING OF INTACT WHOLE TISSUES
    10.
    发明申请
    METHODS FOR PHENOTYPING OF INTACT WHOLE TISSUES 审中-公开
    用于定位全身组织的方法

    公开(公告)号：WO2015041755A1

    公开(公告)日：2015-03-26

    申请号：PCT/US2014/048985

    申请日：2014-07-30

    申请人： CALIFORNIA INSTITUTE OF TECHNOLOGY

    发明人： GRADINARU, Viviana ,  YANG, Bin

    IPC分类号： G01N33/533

    CPC分类号： G01N1/30 ,  G01N33/57492 ,  G01N2001/302 ,  G01N2001/305

    摘要： In various embodiments, the present application teaches methods and compositions for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically-transparent, thereby exposing their cellular structure with intact connectivity. In some embodiments, the present application teaches PACT, a protocol for passive tissue clearing and immunostaining of intact organs. In other embodiments, the present application teaches RIMS, a refractive index matching media for imaging thick tissue. In yet other embodiments, the application teaches PARS, a method for whole-body clearing and immunolabeling.

    摘要翻译： 在各种实施方案中，本申请教导了组织清除的方法和组合物，其中整个器官和身体被赋予大分子可渗透性和光学透明性，从而以完整的连接性暴露其细胞结构。 在一些实施方案中，本申请教导了PACT，用于完整器官的被动组织清除和免疫染色的方案。 在其他实施例中，本申请教导了RIMS，用于成像厚组织的折射率匹配介质。 在其它实施方案中，该应用教导了PARS，全身清除和免疫标记的方法。