公开(公告)号：WO2014201088A1

公开(公告)日：2014-12-18

申请号：PCT/US2014/041863

申请日：2014-06-11

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： AKKUS, Ozan ,  SLIPCHENKO, Mikhail ,  AKKUS, Anna ,  YANG, Shan ,  LI, Bolan

IPC: G01J3/44 ,  G01N21/62 ,  G01N21/65 ,  G01N25/14

CPC classification number: C12Q1/40 ,  C12Q1/37 ,  G01N1/4077 ,  G01N21/65 ,  G01N2001/4088 ,  G01N2201/121 ,  G01N2333/928 ,  G01N2333/958 ,  G01N2800/107 ,  G01N2800/345

Abstract: Methods for determining whether certain compounds, in particular crystals, are present in a sample of a biological fluid that indicates an individual has a particular disease or condition, such as but not limited to gout, pseudogout or urinary tract stones. In some embodiments, the methods include the steps of digestion and filtration of a sample of synovial fluid in order to isolate, if present, monosodium urate monohydrate (MSU), calcium pyrophosphate dihydrate (CPPD), or calcium phosphate crystals from the sample, wherein the filtrate is analyzed with a Raman device to ascertain the presence and type of the crystals. Devices for performing steps of the method are disclosed.

Abstract translation: 用于确定某些化合物，特别是晶体是否存在于指示个体的生物流体样品中具有特定疾病或病症（例如但不限于痛风，假结肠或泌尿道结石）的方法。 在一些实施方案中，所述方法包括以下步骤：消化和过滤滑液样品，以便从样品中分离（如果存在的话）单酸一钠水溶液（MSU），焦磷酸钙二水合物（CPPD）或磷酸钙晶体，其中 用拉曼装置分析滤液以确定晶体的存在和类型。 公开了用于执行该方法的步骤的装置。