公开(公告)号：WO2023077158A1

公开(公告)日：2023-05-04

申请号：PCT/US2022/079062

申请日：2022-11-01

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： GURKAN, Umut ,  AN, Ran ,  SEKYONDA, Zoe ,  FRAIWAN, Arwa

IPC: C12Q1/6806 ,  B01L3/00 ,  G01N27/447 ,  G01N33/72

Abstract: Embodiments described herein relate to a diagnostic system and electrophoresis device for detecting and/or measuring hemoglobin variants in blood of a subject. The diagnostic system includes a cartridge and a UV imaging system. The cartridge includes a housing having a microchannel extending between first and second buffer pools each containing a buffer solution. The buffer solution exhibits an affinity to non-glycosylated hemoglobin, which facilitates its separation from glycosylated hemoglobin. An electrophoresis strip is positioned within the microchannel and is structured to receive at least a portion of a hemolysate. The electrophoresis strip has first and second ends positioned in the first and second buffer pools so as to be at least partially saturated with the buffer solution in each buffer pool, with a first electrode exposed to the buffer solution in the first buffer pool, and a second electrode exposed to the buffer solution in the second buffer pool.

公开(公告)号：WO2022246326A2

公开(公告)日：2022-11-24

申请号：PCT/US2022/030561

申请日：2022-05-23

Applicant: CASE WESTERN RESERVE UNIVERSITY

Inventor： GURKAN, Umut ,  SEKYONDA, Zoe ,  AKKUS, Ozan ,  AN, Ran

IPC: A61B5/145 ,  A61B5/00 ,  A61B10/00 ,  G01N2800/22 ,  G01N33/49 ,  G01N33/721

Abstract: A method of determining at least one of hemoglobin oxygen affinity, rate of hemoglobin deoxygenation, or the presence of hemoglobin variants in blood of a subject, the method includes determining differences of absorption spectra of oxygenated and deoxygenated hemoglobin, red blood, and/or blood obtained from the subject and comparing the determined absorption spectra differences to a control value, wherein the absorption spectra differences are indicative of hemoglobin oxygen affinity, rate of hemoglobin deoxygenation, or the presence of hemoglobin variants in the blood of the subject.