公开(公告)号：WO9909042A3

公开(公告)日：1999-05-06

申请号：PCT/US9816870

申请日：1998-08-13

Applicant: CEPHEID

Inventor： CHRISTEL LEE ALLAN ,  KOVACS GREGORY T A ,  MCMILLAN WILLIAM A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  POURAHMADI FARZAD

IPC: G01N31/20 ,  B01D15/08 ,  B01F5/04 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C07H1/00 ,  C07H21/00 ,  G01N1/34 ,  G01N27/447 ,  G01N33/53 ,  G01N37/00 ,  C12M1/26

CPC classification number: B01L3/502753 ,  B01F5/0403 ,  B01F5/0645 ,  B01F13/0059 ,  B01J19/0093 ,  B01L3/502707 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/086 ,  C07H1/00 ,  G01N1/405

Abstract: The invention presents a microfluidic device and method for separating a desired material, such as nucleic acid, from other materials in a fluid sample. In a preferred embodiment, the device comprises a microfabricated chip (20) having an inlet port (28), an outlet port (30), and an extraction chamber (26) in fluid communication with the ports. The chamber (26) has internal attachment surfaces for capturing the desired material from the fluid sample as the sample flows continuously through the chamber. The captured material may then be eluted by forcing an elution fluid to flow through the chamber (26), thus releasing the material from the internal surfaces into the elution fluid. The flow-through design of the device allows target material from a relatively large volume of fluid sample to be concentrated into a much smaller volume of elution fluid. The internal surfaces are preferably formed by an array of columns (32) integrally formed with a wall of the chamber (26) and extending into the chamber (26). The collumns (32) provide a large surface area for capturing the desired material. The device also preferably includes an integrated heater (34) for increasing elution efficiency.

Abstract translation: 本发明提出了一种微流体装置和方法，用于从流体样本中的其他材料中分离出所需材料，如核酸。 在一个优选实施例中，该装置包括具有与端口流体连通的入口端口（28），出口端口（30）和抽取腔室（26）的微制造芯片（20）。 腔室（26）具有内部连接表面，用于在样品连续流过腔室时从流体样品中捕获所需材料。 然后可以通过迫使洗提流体流过室（26）来洗提捕获的材料，从而将材料从内表面释放到洗提流体中。 该装置的流通式设计允许来自相对较大体积的流体样品的目标物质被浓缩成小得多的洗脱液体积。 内表面优选由与腔室（26）的壁整体形成并延伸到腔室（26）中的一列柱（32）形成。 柱（32）为捕获所需材料提供了大的表面积。 该设备还优选包括用于提高洗脱效率的集成加热器（34）。