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    UNITARY CHEMICAL PROCESSING DEVICE
    1.
    发明申请
    UNITARY CHEMICAL PROCESSING DEVICE 审中-公开
    单位化学处理装置

    公开(公告)号：WO9947255A8

    公开(公告)日：2001-07-19

    申请号：PCT/US9906150

    申请日：1999-03-17

    Applicant: CEPHEID

    Inventor： NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  KOVACS GREGORY T A

    IPC: B03C5/00 ,  B01D57/02 ,  B01J19/00 ,  B01L3/00 ,  B01L99/00 ,  G01N27/447 ,  G01N30/60

    CPC classification number: G01N27/44743 ,  B01J19/0093 ,  B01J2219/00853 ,  B01J2219/00873 ,  B01J2219/00889 ,  B01J2219/00905 ,  B01J2219/00909 ,  B01J2219/00912 ,  B01J2219/00952 ,  B01J2219/0097 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2300/0681 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2300/1883 ,  B01L2400/0406 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/0655 ,  G01N30/6095

    Abstract: A sample-processing device (150) comprises a unitary body (152), preferably a molded polymeric part, having formed therein a reaction chamber (154) for chemically reacting a sample, a separation region (158) for separating components of the sample, and a transition region (156) connecting the reaction chamber (154) to the separation region (158). The reaction chamber (154), transition region (156), and separation region (158) are formed in and enclosed by the unitary body (152). Additionally, the transition region (156) includes at least one flow restrictor (180) for controlling the flow of fluid between the reaction chamber (154) and the separation region (158). Further, the portion of the unitary body (152) defining the transition region (156) has lower thermal conduction than the portion of the body defining the reaction chamber (154) so that the transition region (156) thermally isolates the reaction chamber (154) from the separation region (156). In a preferred embodiment, the reaction chamber (154) is an amplification chamber for amplifying nucleic acid in the sample, and the separation region (158) comprises an electrophoresis column or capillary containing a suitable matrix material, such as electrophoresis gel or buffer, for separating nucleic acid fragments in the sample. Electrodes (167, 168, 169) are embedded in the body (152) for forcing the sample to flow from the reaction chamber (154) to the separation region (158). The unitary body (152) may also be surrounded by external, functional components such as an optical detector (186) for detecting separated components of the sample.

    Abstract translation: 样品处理装置（150）包括整体（152），优选为模制聚合物部分，其中形成有用于使样品化学反应的反应室（154），用于分离样品组分的分离区域（158） 以及将反应室（154）连接到分离区域（158）的过渡区域（156）。 反应室（154），过渡区域（156）和分离区域（158）由整体（152）形成并包围。 另外，过渡区域（156）包括用于控制反应室（154）和分离区域（158）之间的流体流动的至少一个限流器（180）。 此外，限定过渡区域（156）的整体（152）的部分具有比限定反应室（154）的主体部分更低的热传导，使得过渡区域（156）将反应室（154）热隔离 ）从分离区域（156）。 在优选的实施方案中，反应室（154）是用于放大样品中的核酸的扩增室，分离区域（158）包含电泳柱或毛细管，其含有合适的基质材料，例如电泳凝胶或缓冲液，用于 分离样品中的核酸片段。 电极（167,168,169）嵌入在主体（152）中，用于迫使样品从反应室（154）流到分离区（158）。 整体（152）也可以被诸如用于检测样品的分离组分的光学检测器（186）的外部功能部件包围。

    MULTI-SITE REACTOR SYSTEM WITH DYNAMIC, INDEPENDENT CONTROL OF INDIVIDUAL REACTION SITES
    5.
    发明申请
    MULTI-SITE REACTOR SYSTEM WITH DYNAMIC, INDEPENDENT CONTROL OF INDIVIDUAL REACTION SITES 审中-公开
    具有独立反应场所独立控制的多场反应器系统

    公开(公告)号：WO9948608A3

    公开(公告)日：1999-12-09

    申请号：PCT/US9906628

    申请日：1999-03-23

    Applicant: CEPHEID

    Inventor： YOUNG STEVEN J ,  KOVACS GREGORY T A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  MCMILLAN WILLIAM A ,  OTHMER KONSTANTIN ,  CHRISTEL LEE A

    IPC: C12Q1/68 ,  B01J19/00 ,  B01L7/00 ,  G05B13/02 ,  G05D23/19

    CPC classification number: B01L7/52 ,  G05B13/021 ,  G05D23/1913

    Abstract: A multi-site reactor system (60) provides dynamic, independent, computer-implemented control of each reaction site, permitting different thermal profiles to be performed on samples at different reaction sites simultaneously. The system includes reaction vessels (2) for holding reaction mixtures and heat-exchanging modules (37) for receiving the vessels. The system also includes a controller (64) for independently controlling each heat-exchanging module (37) to heat and/or cool the reaction mixture contained therein. The controller (64) includes an adaptive control program for dynamically adjusting the duration or intensity of power pulses provided to each heating element and for dynamically adjusting the duration of fan operation to attain target temperatures quickly and accurately without overshooting or undershooting the target.

    Abstract translation: 多位点反应器系统（60）为每个反应位点提供动态，独立的计算机实现的控制，允许在不同反应位点的样品上同时进行不同的热分布。 该系统包括用于保持反应混合物的反应容器（2）和用于接收容器的热交换模块（37）。 该系统还包括用于独立地控制每个热交换模块（37）以加热和/或冷却其中包含的反应混合物的控制器（64）。 控制器（64）包括一个自适应控制程序，用于动态地调整提供给每个加热元件的功率脉冲的持续时间或强度，并且用于动态地调整风扇操作的持续时间以快速且准确地达到目标温度，而不会超标或低于目标。

    MULTIPLE BEAD REAGENT SYSTEM FOR PROTEIN BASED ASSAYS WITH OPTIMIZED MATRICES
    6.
    发明申请
    MULTIPLE BEAD REAGENT SYSTEM FOR PROTEIN BASED ASSAYS WITH OPTIMIZED MATRICES 审中-公开
    具有优化基质的蛋白质多样性测试系统

    公开(公告)号：WO2006036848A3

    公开(公告)日：2009-04-02

    申请号：PCT/US2005034310

    申请日：2005-09-23

    Applicant: CEPHEID ,  MCMILLAN WILLIAM A ,  MOON BYUNG SOOK ,  JONES MARTIN

    Inventor： MCMILLAN WILLIAM A ,  MOON BYUNG SOOK ,  JONES MARTIN

    IPC: C12Q1/68 ,  G01N33/53

    CPC classification number: G01N33/54393 ,  C12Q1/6846 ,  C12Q2547/107 ,  C12Q2545/101 ,  C12Q2527/125

    Abstract: The invention provides a multi-bead assay system for a protein based assay comprising at least two different beads. The first bead comprises protein and a protein stabilization matrix. The first bead forms a first solution when dissolved in liquid, and the first solution permts a first activity level for the assay. The second bead comprises a potentiation bead matrix that when dissolved in the first solution forms a second solution that potentiates the protein based assay to achieve a second actitivy level that is higher than the first activity level.

    Abstract translation: 本发明提供了一种用于基于蛋白质的测定的多珠测定系统，其包含至少两种不同的珠粒。 第一珠包含蛋白质和蛋白质稳定基质。 当溶解在液体中时，第一珠粒形成第一溶液，并且第一溶液将第一活性水平用于测定。 第二珠粒包含增强珠粒基质，其当溶解在第一溶液中时形成第二溶液，其加强基于蛋白质的测定以达到高于第一活性水平的第二活性水平。

    MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES
    7.
    发明申请
    MICROSTRUCTURES FOR THE MANIPULATION OF FLUID SAMPLES 审中-公开
    用于操纵流体样本的微结构

    公开(公告)号：WO9909042A3

    公开(公告)日：1999-05-06

    申请号：PCT/US9816870

    申请日：1998-08-13

    Applicant: CEPHEID

    Inventor： CHRISTEL LEE ALLAN ,  KOVACS GREGORY T A ,  MCMILLAN WILLIAM A ,  NORTHRUP M ALLEN ,  PETERSEN KURT E ,  POURAHMADI FARZAD

    IPC: G01N31/20 ,  B01D15/08 ,  B01F5/04 ,  B01F5/06 ,  B01F13/00 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  C07H1/00 ,  C07H21/00 ,  G01N1/34 ,  G01N27/447 ,  G01N33/53 ,  G01N37/00 ,  C12M1/26

    CPC classification number: B01L3/502753 ,  B01F5/0403 ,  B01F5/0645 ,  B01F13/0059 ,  B01J19/0093 ,  B01L3/502707 ,  B01L2200/0631 ,  B01L2200/0636 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2300/1827 ,  B01L2400/0421 ,  B01L2400/0487 ,  B01L2400/086 ,  C07H1/00 ,  G01N1/405

    Abstract: The invention presents a microfluidic device and method for separating a desired material, such as nucleic acid, from other materials in a fluid sample. In a preferred embodiment, the device comprises a microfabricated chip (20) having an inlet port (28), an outlet port (30), and an extraction chamber (26) in fluid communication with the ports. The chamber (26) has internal attachment surfaces for capturing the desired material from the fluid sample as the sample flows continuously through the chamber. The captured material may then be eluted by forcing an elution fluid to flow through the chamber (26), thus releasing the material from the internal surfaces into the elution fluid. The flow-through design of the device allows target material from a relatively large volume of fluid sample to be concentrated into a much smaller volume of elution fluid. The internal surfaces are preferably formed by an array of columns (32) integrally formed with a wall of the chamber (26) and extending into the chamber (26). The collumns (32) provide a large surface area for capturing the desired material. The device also preferably includes an integrated heater (34) for increasing elution efficiency.

    Abstract translation: 本发明提出了一种微流体装置和方法，用于从流体样本中的其他材料中分离出所需材料，如核酸。 在一个优选实施例中，该装置包括具有与端口流体连通的入口端口（28），出口端口（30）和抽取腔室（26）的微制造芯片（20）。 腔室（26）具有内部连接表面，用于在样品连续流过腔室时从流体样品中捕获所需材料。 然后可以通过迫使洗提流体流过室（26）来洗提捕获的材料，从而将材料从内表面释放到洗提流体中。 该装置的流通式设计允许来自相对较大体积的流体样品的目标物质被浓缩成小得多的洗脱液体积。 内表面优选由与腔室（26）的壁整体形成并延伸到腔室（26）中的一列柱（32）形成。 柱（32）为捕获所需材料提供了大的表面积。 该设备还优选包括用于提高洗脱效率的集成加热器（34）。

    MULTIPLEXED DETECTION OF BIOLOGICAL AGENTS
    9.
    发明申请
    MULTIPLEXED DETECTION OF BIOLOGICAL AGENTS 审中-公开
    多重检测生物代理

    公开(公告)号：WO2005111243A3

    公开(公告)日：2007-03-01

    申请号：PCT/US2005016257

    申请日：2005-05-06

    Applicant: CEPHEID ,  MCMILLAN WILLIAM A

    Inventor： MCMILLAN WILLIAM A

    IPC: C12Q1/68 ,  C12N15/11 ,  G01N33/53 ,  G01N33/68

    CPC classification number: G01N33/68 ,  C12Q1/6816 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2563/107

    Abstract: Described are methods, kits and systems for multiplexed detection of biological agents in a sample, e.g., multiplexed detection of e.g., bacteria, viruses, and biological toxins. The method utilizes two markers for each agent; the presence or absence in a sample of each ~f the two markers per agent is determined in separate reactions; however each reaction is used to detect a single marker for multiple agents. Also disclosed is the multiplexed detection method using real time PCR. The invention provides an efficient, cost-effective, and specific method for multiplexed detection of biological agents.

    Abstract translation: 描述了用于多样化检测样品中生物制剂的方法，试剂盒和系统，例如多重检测例如细菌，病毒和生物毒素。 该方法为每个试剂使用两个标记; 每个试剂每个样品的存在或不存在每个试剂的两个标记在单独的反应中确定; 然而，每个反应用于检测多个试剂的单个标记物。 还公开了使用实时PCR的多路复用检测方法。 本发明提供了生物制剂多重检测的有效，成本有效和具体的方法。

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