公开(公告)号：WO2023023359A1

公开(公告)日：2023-02-23

申请号：PCT/US2022/040939

申请日：2022-08-19

Applicant: CEPHEID

Inventor： SHAH, Amish ,  LEE, Marissa ,  KOAY, Jessica ,  CASLER JR., Richard J.

IPC: G01N21/64 ,  H05B45/14

Abstract: Systems and methods for monitoring, characterizing and controlling operation of LEDs are provided herein. Methods include measuring a voltage (V) across a LED (1), and correlating the voltage (V) to a junction temperature of the LED (1). This correlation can be used to improve operation of the LED (1) by increasing the signal to noise ratio of the LED signal (5), characterize the LED (1) by comparing to an l-V curve, control LED operation to compensate for LED degradation and avoid crosstalk, and/or to generally improve performance and life expectancy of the LED (1). Improved performance of the LED (1) can include stabilizing the photon output (5) during performance of an assay to provide a desired dye reporter signal required for the assay and/or reducing an intra-shot during of the LED output (5) during the assay. System and device with control units (2) configured to perform these methods are also described herein.

公开(公告)号：WO2022155304A1

公开(公告)日：2022-07-21

申请号：PCT/US2022/012275

申请日：2022-01-13

Applicant: CEPHEID ,  ROHRS, Charles

Inventor： LEE, Marissa ,  KOAY, Jessica ,  SOLIS, Earl ,  PICCINI, Matthew ,  CASLER JR., Richard J.

IPC: G05D23/19 ,  B01L7/00 ,  B01L1/025 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L7/04 ,  B01L7/52 ,  B01L9/06 ,  F25B21/02 ,  G01K1/026 ,  G01K13/024 ,  G01N1/42 ,  G01N1/44 ,  G01N2035/00346 ,  G05D23/1919 ,  G05D23/1931

Abstract: Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.