公开(公告)号：WO2022237937A1

公开(公告)日：2022-11-17

申请号：PCT/DE2022/100350

申请日：2022-05-06

Applicant: 3P INSTRUMENTS GMBH & CO. KG

Inventor： ESCHRICH, Robert ,  KLANK, Dietmar ,  SEIDLER, Andreas ,  GRAF, Matthias

IPC: B01L7/00 ,  F25D3/10 ,  G01N15/08 ,  B01L2300/0854 ,  B01L2300/1822 ,  B01L2300/1883 ,  B01L2300/1894 ,  B01L7/52

Abstract: Die Erfindung betrifft eine Vorrichtung (1a, 1 b) zum Temperieren einer Messzelle (30), insbesondere einer Gassorptions-Messzelle, mit einem isolierten Gefäß (4) zur Aufnahme eines Kühlmediums und einer Messapparatur. Die Messapparatur weist ein Temperierelement (8) mit einer Aufnahmeöffnung (9) zur Aufnahme der Messzelle (30) und eine Kühlvorrichtung zum Temperieren des Temperierelements (8) auf, welche in Richtung einer Längsachse (L) ausgerichtet und wärmeübertragend aneinander anliegen. Die Kühlvorrichtung ist mit einem Befestigungselement (15a, 15b) zum Fixieren des Temperierelements (8) an einer Wandung des Gefäßes (4) und zur Wärmeübertragung zwischen der Messapparatur und dem Kühlmedium ausgebildet. Zwischen dem Temperierelement (8) und der Kühlvorrichtung ist in Richtung der Längsachse (L) ein thermoelektrisches Kühlelement (13) zum Kühlen des Temperierelements (8) und der Messzelle (30) angeordnet. Die Erfindung betrifft zudem ein Verfahren zum Betreiben der Vorrichtung (1a, 1 b) zum Temperieren der Messzelle (30).

公开(公告)号：WO2022195289A2

公开(公告)日：2022-09-22

申请号：PCT/GB2022/050686

申请日：2022-03-18

Applicant: BG RESEARCH LTD

Inventor： NAZARETH, Nelson

IPC: B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  B65D17/00 ,  B04B15/02 ,  B01L2300/044 ,  B01L2300/0809 ,  B01L2300/1822 ,  B01L2400/0409 ,  B01L7/52 ,  G01N2035/00346

Abstract: An apparatus comprising: first and second Peltier devices arranged in substantial opposition to one another; and a thermally conducting chamber defined substantially between the first and second Peltier devices and configured to enclose a reaction vessel during use to facilitate a transfer of heat between the reaction vessel and the Peltier devices.

公开(公告)号：WO2020257356A2

公开(公告)日：2020-12-24

申请号：PCT/US2020/038242

申请日：2020-06-17

Applicant: MAMMOTH BIOSCIENCES, INC. ,  BROUGHTON, James Paul ,  SINGH, Jasmeet ,  FASCHING, Clare Louise ,  TSALOGLOU, Maria-Nefeli ,  GALARZO, Pedro Patrick Draper ,  CHEN, Janice Sha ,  MIAO, Xin ,  HARRINGTON, Lucas ,  DRZAL, Daniel Thomas ,  SHAPIRO, Sarah Jane

Inventor： BROUGHTON, James Paul ,  SINGH, Jasmeet ,  FASCHING, Clare Louise ,  TSALOGLOU, Maria-Nefeli ,  GALARZO, Pedro Patrick Draper ,  CHEN, Janice Sha ,  MIAO, Xin ,  HARRINGTON, Lucas ,  DRZAL, Daniel Thomas ,  SHAPIRO, Sarah Jane

IPC: B01L3/00 ,  B01L7/00 ,  B01L2200/0605 ,  B01L2200/0689 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0487 ,  B01L2400/0644 ,  B01L2400/065 ,  B01L2400/0655 ,  B01L2400/0666 ,  B01L3/502738 ,  B01L7/525

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids.

公开(公告)号：WO2023057338A1

公开(公告)日：2023-04-13

申请号：PCT/EP2022/077322

申请日：2022-09-30

Applicant: BAYER AKTIENGESELLSCHAFT

Inventor： SCHADE, Andreas ,  KÜSTER, Mike ,  OCHMANN, Klaus ,  PFLÜGER, Philipp ,  GESELL, Robin ,  NICOL, Andreas ,  FELLER, Torsten ,  SUMMER, Holger ,  GOLZ, Stefan ,  KALTHOF, Bernd

IPC: B01L3/00 ,  B01L7/00 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L3/50851 ,  B01L7/5255

Abstract: : The invention relates to a system comprising at least one disposable comprising one or more wells in a body, wherein the wells are capable of acting as vessel for one or more chemical, biochemical or molecular biological reactions requiring one or more prescribed reaction temperatures according to a prescribed protocol, said body comprising a flat bottom side building a first heating surface capable of homogeneously conducting heat into the wells and a flat upper side comprising well openings sealed by means of a transparent sealing foil building a second heating surface capable of homogeneously conducting heat in the wells, said body being arranged in a rigid frame support; one or more separate tempering units TUx, each tempering unit TUx comprising at least one first temperature-controlled plates with a planar seat to conform to the flat bottom side of the disposable; transport means comprising a carrier in which the disposable is placed and a moving mechanism for moving the disposable into and/or out of the tempering unit TUx and for contacting one of the heating surfaces of the disposable with the first temperature-controlled plates; a control unit comprising one or more processors configured to activate the tempering units TUx and the transport means for the one or more chemical, biochemical, or molecular biological reactions. The solution of the present invention is particularly adapted for high throughput reactions or assay.

公开(公告)号：WO2022178488A2

公开(公告)日：2022-08-25

申请号：PCT/US2022/070542

申请日：2022-02-05

Applicant: CYTEK BIOSCIENCES, INC.

Inventor： KRUEGER, Glen ,  VRANE, David

IPC: G01N15/14 ,  G01N21/49 ,  G01N21/63 ,  B01L2200/0652 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1844 ,  B01L2300/1894 ,  B01L3/0241 ,  B01L3/502715 ,  B01L3/502761 ,  C12M47/04 ,  G01N15/1436 ,  G01N2015/0065 ,  G01N2015/1006 ,  G01N2015/1409 ,  G01N2015/149

Abstract: A flow cytometry or cell sorting system is disclosed. Under pressure, a fluidics system causes sheath and sample biological fluids to flow. The fluidics system can include a degasser. A flow cell communicates with the fluidics system to receive the sheath fluid, wherein a sample biological fluid flows with cells or particles through the flow cell to be surrounded by the sheath fluid. A deflection chamber under the flow cell receives the drops of sample biological fluid and sheath fluid out of the flow cell to selectively deflect one or more of the drops along one or more deflection paths; and a droplet deposition unit to receive selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers.

公开(公告)号：WO2021257348A1

公开(公告)日：2021-12-23

申请号：PCT/US2021/036658

申请日：2021-06-09

Applicant: BIO-RAD LABORATORIES, INC.

Inventor： BAUTISTA, Garret

IPC: C12Q1/686 ,  B01L3/00 ,  B01L7/00 ,  B01L2300/046 ,  B01L2300/0663 ,  B01L2300/123 ,  B01L2300/16 ,  B01L2300/1822 ,  B01L2300/1833 ,  B01L2300/1883 ,  B01L3/50851 ,  B01L7/52

Abstract: A sample plate for a thermal cycler suitable for performing a polymerase chain reaction (PCR) procedure includes a base plate and a number of reaction vessels extending upward from the base plate. The sample plate further includes a vertical wall surrounding an outer perimeter defined by the reaction vessels. The vertical wall can be a continuation vertical wall, an intermittent vertical wall, or a perforated vertical wall. The intermittent vertical wall can include a plurality of wall portions, each of which plurality of wall portions is separated from other wall portions via a plurality of gaps.

公开(公告)号：WO2022155304A1

公开(公告)日：2022-07-21

申请号：PCT/US2022/012275

申请日：2022-01-13

Applicant: CEPHEID ,  ROHRS, Charles

Inventor： LEE, Marissa ,  KOAY, Jessica ,  SOLIS, Earl ,  PICCINI, Matthew ,  CASLER JR., Richard J.

IPC: G05D23/19 ,  B01L7/00 ,  B01L1/025 ,  B01L2200/147 ,  B01L2200/148 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/1822 ,  B01L2300/1894 ,  B01L7/04 ,  B01L7/52 ,  B01L9/06 ,  F25B21/02 ,  G01K1/026 ,  G01K13/024 ,  G01N1/42 ,  G01N1/44 ,  G01N2035/00346 ,  G05D23/1919 ,  G05D23/1931

Abstract: Thermal control devices and methods to provide improved control, speed and efficiency in temperature cycling are provided herein. Such thermal control device and methods can include one or more active elements, such a thermoelectric cooler device, that is controlled by an algorithm that regulates a temperature distribution of an adjacent reaction-vessel according to a temperature distribution command trajectory and estimated reaction-vessel temperature distribution. Some embodiments include two active elements that are bilaterally applied to opposing sides of the reaction-vessel. In some embodiments, the estimated reaction-vessel temperature is determined based on a state of power electronics of the element and a temperature output of one or more sensors of a portion of the element and/or an ambient environment of the reaction-vessel. Methods of calibration of such systems utilizing a thermal calibrator as a proxy for the reaction-vessel are also provided herein.

公开(公告)号：WO2021220192A2

公开(公告)日：2021-11-04

申请号：PCT/IB2021/053530

申请日：2021-04-28

Applicant: STAB VIDA - INVESTIGAÇÃO E SERVIÇOS EM CIÊNCIAS BIOLÓGICAS, LDA

Inventor： DE LEÃO E FLORES, Alcino Orfeu ,  REIMÃO PINTO DE FRANÇA DORIA, Gonçalo Maria

IPC: B01L7/00 ,  C12Q1/68 ,  C12Q1/70 ,  B01L2300/023 ,  B01L2300/0663 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L7/52 ,  C12Q1/701

Abstract: Present invention refers to method and portable device(1) for detection and identification of specific sequences of nucleic acids in different samples, by utilisation of reverse transcription technique and/or isothermal amplification, using specific oligonucleotide primers of target region(s) to detect, whereby the amplification of the same is conducted in indirect and non- specific form, or direct and specific, using discriminatory amplification reagents, which signal, colourimetric/fluorescent, is recorded by device (1) where reaction is carried out. Device is controlled by a mobile application(9), also recording data acquired by device (1) analyses, stores it in remote server in cloud (10), which analyses global set of all data allowing global analysis of for example, evolution of epidemics. Invention is useful for detection and identification of relevant nucleic sequences for different types of diagnostic in different clinical, pharmaceutical, veterinary, food, environmental, biotechnological, biosafety areas, particularly useful in quick, low-cost diagnostics, and at point of care, of SARS-CoV-2 virus.

公开(公告)号：WO2021198476A1

公开(公告)日：2021-10-07

申请号：PCT/EP2021/058731

申请日：2021-04-01

Applicant: DIASCREEN GMBH

Inventor： MISEREZ, André, Roger ,  LUX, Angela, Mirela

IPC: B01L3/00 ,  B01L7/00 ,  B01L2200/147 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2400/0478 ,  B01L2400/0616 ,  B01L2400/0644 ,  B01L3/502 ,  B01L3/502738 ,  B01L7/52

Abstract: The invention is notably directed to a point-of-care diagnostic device (1). The latter includes a fluid-mixing compartment (10) with a conduit (15), a fluid-tight piston (20), and two chambers (11, 13). The piston is slidably movable in the conduit. The chambers comprise a first chamber (11) and a second chamber (13), each communicating with the conduit via a first valve (115) and a second valve (135), respectively. Each of the first valve and the second valve is configured to be actuated by the piston, in operation. The device further includes a reaction chamber (30), which is in fluidic communication with the conduit. The device is configured to controllably move the piston in the conduit, so as to successively actuate the first valve and the second valve, and then adjust the pressure in the reaction chamber for performing a reaction therein. Actuating the first valve allows a liquid sample that initially is in the first chamber to enter the conduit. Actuating the second valve allows contents thereof to admix with the liquid sample, such that an admixture can eventually be obtained in the reaction chamber, which is pressure-adjusted (by controllably moving the piston in the conduit) for performing said reaction. The invention is further directed to related methods of operation.

公开(公告)号：WO2021127315A1

公开(公告)日：2021-06-24

申请号：PCT/US2020/065810

申请日：2020-12-18

Applicant: BECKMAN COULTER, INC.

Inventor： DAVIS, Matthew S. ,  LU, Kristina K. ,  MOSCHELL, Rachel Ellen ,  NEI, Peter Robert ,  SAUERBURGER, Mark F ,  SMITH, Zachary M. ,  SNIDER, John S. ,  SPRINGSTON, Jason L.

IPC: B01L3/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N35/00 ,  B01L2200/023 ,  B01L2200/147 ,  B01L2300/021 ,  B01L2300/0858 ,  B01L2300/1822 ,  B01L2400/043 ,  B01L3/5082 ,  B01L7/54 ,  B01L9/06 ,  C12Q1/6846

Abstract: A reaction vessel comprises a lower chamber with a first volume, and an upper chamber with a second volume greater than the first volume. A thermocycling system for heating the reaction vessel includes a lower heating zone to heat the lower chamber, an upper heating zone to heat the upper chamber, and a lid heater to heat an opening of the upper chamber. A method comprises loading a sample into a lower chamber of a reaction vessel, thermocycling the lower chamber using a lower heating zone of the thermo cycler, combining an additive into the sample to produce a combination filling the lower chamber and at least partially filling an upper chamber of the reaction vessel, and incubating the upper and lower chambers using the lower heating zone and an upper heating zone. The lower and upper chambers can have different wall thicknesses to facilitate heat transfer.