公开(公告)号：WO2019005337A1

公开(公告)日：2019-01-03

申请号：PCT/US2018/034073

申请日：2018-05-23

申请人： CODEXIS, INC.

发明人： VOLADRI, Rama ,  MICKLITSCH, Christopher, Michael ,  ALVIZO, Oscar ,  NAZOR, Jovana ,  DUAN, Da ,  VIDUYA, Judy, Victoria Antonio ,  JENNE, Stephan ,  AN, Chihui ,  CANADA, Keith, Allen ,  DEVINE, Paul, N. ,  FARASAT, Iman ,  FRYSZKOWSKA, Anna ,  LEXA, Katrina, W. ,  ORR, Robert, Kevin

IPC分类号： C12N9/80 ,  C12N9/84 ,  C12N15/09 ,  C12N15/55

摘要： The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions comprising the enzymes, and methods of using the engineered PGA enzymes.

公开(公告)号：WO2020150125A1

公开(公告)日：2020-07-23

申请号：PCT/US2020/013288

申请日：2020-01-13

申请人： CODEXIS, INC.

发明人： NAZOR, Jovana ,  MICKLITSCH, Christopher, Michael ,  DUAN, Da ,  SOWELL-KANTZ, Auric, Anthony ,  MILLER, Steven ,  KOSJEK, Birgit ,  FARASAT, Iman ,  MARSHALL, Nicholas, M.

IPC分类号： C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/63 ,  C12P13/04 ,  C12Q1/52

摘要： The present invention provides engineered transaminase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered transaminase polypeptides. Methods for producing transaminase enzymes are also provided. In some embodiments, the engineered transaminase polypeptides are optimized to provide improved enzyme properties under minimal aqueous conditions in wet organic solvents.

公开(公告)号：WO2019118557A1

公开(公告)日：2019-06-20

申请号：PCT/US2018/065124

申请日：2018-12-12

申请人： CODEXIS, INC.

发明人： VOLADRI, Rama ,  ENTWISTLE, David ,  MICKLITSCH, Christopher, Michael ,  xMITCHELL, Vesna ,  DELLAS, Nikki ,  ZHANG, Xiyun ,  SERO, Antoinette ,  DORR, Brent ,  FUERST, Douglas, E.

IPC分类号： C12N9/18 ,  A23L3/3571

摘要： The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.

公开(公告)号：WO2020014049A1

公开(公告)日：2020-01-16

申请号：PCT/US2019/040376

申请日：2019-07-02

申请人： CODEXIS, INC.

发明人： MICKLITSCH, Christopher, Michael ,  ALVIZO, Oscar ,  NAZOR, Jovana ,  MANIAR, Harvinder, Chagger ,  KRAWCZYK, Mikayla, Jianghongxia ,  BORRA-GARSKE, Margie, Tabuga ,  SUBRAMANIAN, Nandhitha ,  FRYSZKOWSKA, Anna ,  MARSHALL, Nicholas, M. ,  RODRIGUEZ-GRANILLO, Agustina ,  VERMA, Deeptak ,  ANDREWS, Dewan

IPC分类号： C12N9/04 ,  C12P21/04

摘要： The present invention provides engineered galactose oxidase (GOase) enzymes, polypeptides having GOase activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing GOase enzymes are also provided. The present invention further provides compositions comprising the GOase enzymes and methods of using the engineered GOase enzymes. The present invention finds particular use in the production of pharmaceutical and other compounds.

公开(公告)号：WO2019118557A8

公开(公告)日：2019-06-20

申请号：PCT/US2018/065124

申请日：2018-12-12

申请人： CODEXIS, INC.

发明人： VOLADRI, Rama ,  ENTWISTLE, David ,  MICKLITSCH, Christopher, Michael ,  MITCHELL, Vesna ,  DELLAS, Nikki ,  ZHANG, Xiyun ,  SERO, Antoinette ,  DORR, Brent ,  FUERST, Douglas, E.

IPC分类号： C12N9/18 ,  A23L3/3571

摘要： The present invention provides engineered carboxyesterase enzymes having improved properties as compared to a naturally occurring wild-type carboxyesterase enzymes, as well as polynucleotides encoding the engineered carboxyesterase enzymes, host cells capable of expressing the engineered carboxyesterase enzymes, and methods of using the engineered carboxyesterase enzymes in amidation reactions.