公开(公告)号：WO2017213758A1

公开(公告)日：2017-12-14

申请号：PCT/US2017/028370

申请日：2017-04-19

Applicant: CODEXIS, INC.

Inventor： NAZOR, Jovana ,  OSBORNE, Robert ,  LIANG, Jack ,  VROOM, Jonathan ,  ZHANG, Xiyun ,  ENTWISTLE, David ,  VOLADRI, Rama ,  GARCIA, Ravi, David ,  MOORE, Jeffrey, C. ,  GROSSER, Shane ,  KOSJEK, Birgit ,  TRUPPO, Matthew

IPC: C12P17/12 ,  C12P13/24

Abstract: The present invention provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents.

Abstract translation: 本发明提供了用于生产羟基化化合物的工程化脯氨酸羟化酶多肽，编码工程化脯氨酸羟化酶的多核苷酸，能够表达工程化脯氨酸羟化酶的宿主细胞，以及使用工程化脯氨酸羟化酶制备 用于生产活性药物的化合物。

公开(公告)号：WO2016130412A1

公开(公告)日：2016-08-18

申请号：PCT/US2016/016692

申请日：2016-02-05

Applicant: CODEXIS, INC.

Inventor： ALVIZO, Oscar ,  AGARD, Nicholas, J. ,  XIE, Xinkai ,  ENTWISTLE, David ,  KOSJEK, Birgit

IPC: C12N9/04 ,  C12P17/10 ,  C12P41/00

CPC classification number: C12N9/0006 ,  C12P7/02 ,  C12P7/40 ,  C12P17/12 ,  C12P41/002 ,  C12Y101/01184

Abstract: The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds.

Abstract translation: 本公开提供与天然存在的野生型酮还原酶相比具有改进性质的工程化酮还原酶。 还提供了编码工程化酮还原酶的多核苷酸，能够表达工程化酮还原酶的宿主细胞，以及使用工程化酮还原酶合成各种手性化合物的方法。

公开(公告)号：WO2013036861A8

公开(公告)日：2013-03-14

申请号：PCT/US2012/054300

申请日：2012-09-07

Applicant: CODEXIS, INC ,  CABIROL, Fabien, L. ,  CHEN, Haibin ,  GOHEL, Anupam ,  SALIM, Paulina ,  SMITH, Derek ,  JANEY, Jacob ,  KOSJEK, Birgit ,  TANG, Weng, Lin ,  HSIEH, Helen ,  PHAM, Son

Inventor： CABIROL, Fabien, L. ,  CHEN, Haibin ,  GOHEL, Anupam ,  SALIM, Paulina ,  SMITH, Derek ,  JANEY, Jacob ,  KOSJEK, Birgit ,  TANG, Weng, Lin ,  HSIEH, Helen ,  PHAM, Son

IPC: C07K14/435

Abstract: The present disclosure relates to transaminase polypeptides capable of aminating a dicarbonyl substrate, and polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides.

公开(公告)号：WO2020150125A1

公开(公告)日：2020-07-23

申请号：PCT/US2020/013288

申请日：2020-01-13

Applicant: CODEXIS, INC.

Inventor： NAZOR, Jovana ,  MICKLITSCH, Christopher, Michael ,  DUAN, Da ,  SOWELL-KANTZ, Auric, Anthony ,  MILLER, Steven ,  KOSJEK, Birgit ,  FARASAT, Iman ,  MARSHALL, Nicholas, M.

IPC: C12N9/10 ,  C12N15/09 ,  C12N15/54 ,  C12N15/63 ,  C12P13/04 ,  C12Q1/52

Abstract: The present invention provides engineered transaminase polypeptides and compositions thereof, as well as polynucleotides encoding the engineered transaminase polypeptides. Methods for producing transaminase enzymes are also provided. In some embodiments, the engineered transaminase polypeptides are optimized to provide improved enzyme properties under minimal aqueous conditions in wet organic solvents.

公开(公告)号：WO2013074650A1

公开(公告)日：2013-05-23

申请号：PCT/US2012/065046

申请日：2012-11-14

Applicant: CODEXIS, INC. ,  CABIROL, Fabien ,  CHEN, Haibin ,  GOHEL, Anupam ,  COLLIER, Steven, J. ,  SMITH, Derek ,  KOSJEK, Birgit ,  JANEY, Jacob

Inventor： CABIROL, Fabien ,  CHEN, Haibin ,  GOHEL, Anupam ,  COLLIER, Steven, J. ,  SMITH, Derek ,  KOSJEK, Birgit ,  JANEY, Jacob

IPC: C12N9/04 ,  C12P13/00 ,  C12P7/00 ,  C12P17/10 ,  C12N9/00

CPC classification number: C12N9/0006 ,  C12N15/70 ,  C12P13/00 ,  C12P13/001 ,  C12P13/02 ,  C12P41/002 ,  C12Y101/01184 ,  Y02P20/52

Abstract: The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.

Abstract translation: 本公开涉及用于制备羟基取代的氨基甲酸酯化合物的工程化酮还原酶多肽，以及多核苷酸，载体，宿主细胞，以及制备和使用酮还原酶多肽的方法。