公开(公告)号：WO2014113208A1

公开(公告)日：2014-07-24

申请号：PCT/US2013/078238

申请日：2013-12-30

Applicant: COSKATA, INC.

Inventor： DATTA, Rathin ,  REEVES, Andrew

IPC: C12N1/21 ,  C12P7/16

CPC classification number: C12N1/20 ,  C12P7/16 ,  Y02E50/10

Abstract: This invention provides compositions for the production of butanol. Specifically, the compositions of the present invention use syntrophic co-cultures for the production of butanol from syngas.

Abstract translation: 本发明提供了用于生产丁醇的组合物。 具体地，本发明的组合物使用合成共培养物从合成气生产丁醇。

公开(公告)号：WO2014160343A1

公开(公告)日：2014-10-02

申请号：PCT/US2014/026350

申请日：2014-03-13

Applicant: COSKATA, INC.

Inventor： REEVES, Andrew

IPC: C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N15/70

CPC classification number: C12N15/74 ,  C12N9/1007 ,  C12N15/63 ,  C12N15/70 ,  C12N15/87 ,  C12Q2521/331 ,  C12Y201/01072

Abstract: This invention provides isolated polynucleotides encoding DNA Type I methyltransferase and uses thereof for improving transformation efficiencies of exogenous and endogenous plasmid DNA into Clostridial hosts.

Abstract translation: 本发明提供编码DNA I型甲基转移酶的分离的多核苷酸及其用于提高外源和内源质粒DNA转化成梭菌宿主的转化效率的用途。

公开(公告)号：WO2011155954A1

公开(公告)日：2011-12-15

申请号：PCT/US2010/043962

申请日：2010-07-30

Applicant: COSKATA, INC. ,  REEVES, Andrew ,  DATTA, Rathin

Inventor： REEVES, Andrew ,  DATTA, Rathin

IPC: C12N15/53 ,  C12N15/63 ,  C12N15/113 ,  C12P7/06

CPC classification number: C12N15/74 ,  C12N15/52 ,  C12P7/065 ,  Y02E50/17

Abstract: Gene sequences of key acetogenic clostridial species were sequenced and isolated. Genes of interest were identified, and functionality was established. Key genes of interest for metabolic catalyzing activity in clostridial species include a three-gene operon coding for CODH activity, a two-gene operon coding for PTA-ACK, and a novel acetyl coenzyme A reductase. The promoter regions of the two operons and the acetyl coA reductase are manipulated to increase ethanol production.

Abstract translation: 对重要的乙型梭菌种的基因序列进行测序和分离。 确定了感兴趣的基因，建立了功能。 梭菌中代谢催化活性的关键基因包括编码CODH活性的三基因操纵子，编码PTA-ACK的双基因操纵子和新型乙酰辅酶A还原酶。 操纵两个操纵子的启动子区域和乙酰辅酶A还原酶以增加乙醇生成。

公开(公告)号：WO2009151584A3

公开(公告)日：2009-12-17

申请号：PCT/US2009/003475

申请日：2009-06-09

Applicant: COSKATA, INC. ,  REEVES, Andrew

Inventor： REEVES, Andrew

IPC: C12Q1/68 ,  G01N27/447 ,  C12N15/11 ,  C12P7/06

Abstract: A method for determining the presence of a contaminating organism in a fermentation process includes initiating the fermentation process using a syngas and obtaining a first aliquot from the fermentation process. The method further includes subjecting said first aliquot to a polymerase chain reaction using at least one oligonucleotide primer capable of hybridizing to a target sequence of a genomic nucleic acid from a suspected contaminating organism, producing a first amplified product based on the polymerase chain reaction, separating the first amplified product based on size, and determining the presence of the suspected contaminating organism based on the first separated amplified product.

公开(公告)号：WO2013043513A1

公开(公告)日：2013-03-28

申请号：PCT/US2012/055612

申请日：2012-09-14

Applicant: COSKATA, INC. ,  DATTA, Rathin ,  REEVES, Andrew ,  KLIMAN, Laura, T.

Inventor： DATTA, Rathin ,  REEVES, Andrew ,  KLIMAN, Laura, T.

IPC: C12P7/16 ,  C12P7/52 ,  C12P7/02 ,  C12P7/40 ,  C12P23/00 ,  C12N9/00 ,  C12N1/20

CPC classification number: C12P7/04 ,  C12P7/065 ,  C12P7/08 ,  C12P7/14 ,  C12P7/52 ,  C12P7/54 ,  C12P39/00 ,  Y02E50/17

Abstract: A method of operating a fermentation zone for the production of ethanol from syngas uses a crotonate-like compound to prevent or reverse the effects of butyrogen contamination. The crotonate-like compound works in continuous fermentation processes to reduce or eliminate contamination from butyrate and butanol in the syngas derived ethanol product.

Abstract translation: 操作用于从合成气生产乙醇的发酵区的方法使用巴豆酸酯化合物来防止或逆转丁酸污染的作用。 巴豆酸酯化合物在连续发酵过程中起作用，以减少或消除合成气衍生乙醇产物中丁酸和丁醇的污染。