公开(公告)号：WO2015059690A8

公开(公告)日：2015-07-23

申请号：PCT/IL2014050902

申请日：2014-10-14

Applicant: YEDA RES & DEV

Inventor： SOREK ROTEM ,  SBERRO HILA ,  GOLDFARB TAMARA

IPC: C12N9/10 ,  C07K14/195

CPC classification number: C12N1/20 ,  A23C9/1234 ,  A23C2220/202 ,  C07K14/195 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0051 ,  C12N9/1007 ,  C12N9/12 ,  C12N9/14 ,  C12N9/16 ,  C12N9/48 ,  C12N15/52 ,  C12Y108/04008 ,  C12Y201/01072 ,  C12Y207/11001 ,  C12Y301/03001 ,  C12Y304/00 ,  C12Y306/04012 ,  C12Y306/04013

Abstract: Isolated polynucleotides encoding a BREX system are provided. Accordingly there is provided an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ and at least one of pglX, pglXI, brxP, brxHI, brxHII, brxL, brxD, brxA, brxB, brxF, and brxE, with the proviso that said BREX system does not comprise pglW, and wherein said BREX system confers phage resistance to a bacteria recombinantly expressing same; Also provided is an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ, pglX, pglW and at least one of brxD and brxHI, and wherein said BREX system confers phage resistance to a bacteria recombinantly expressing same. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.

Abstract translation: 提供编码BREX系统的分离的多核苷酸。 因此，提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ和pglX，pglXI，brxP，brxHI，brxHII，brxL，brxD，brxA，brxB，brxD，brxA，brxB，brxF中的至少一种的BREX系统的核酸序列 和brxE，条件是所述BREX系统不包含pglW，并且其中所述BREX系统赋予对重组表达的细菌的噬菌体抗性; 还提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ，pglX，pglW和至少一种brxD和brxHI的BREX系统的核酸序列，并且其中所述BREX系统将重组的噬菌体抗性赋予细菌 表达相同。 还提供了赋予细菌噬菌体抗性或赋予细菌对噬菌体易感性的组合物和方法。

公开(公告)号：WO2016030546A1

公开(公告)日：2016-03-03

申请号：PCT/EP2015/069879

申请日：2015-08-31

Applicant: KATHOLIEKE UNIVERSITEIT LEUVEN

Inventor： NEELY, Robert ,  LEEN, Volker ,  HOFKENS, Johan

IPC: C07H19/167 ,  C12Q1/68 ,  C12N9/10 ,  A61K31/7076

CPC classification number: C07H19/16 ,  C07H19/167 ,  C12N9/1007 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Y201/01072

Abstract: Cofactor analogues for methyltransferases are disclosed. The compounds are represented by formula (I) wherein R1 is COOH or COO-; X is an organic or inorganic anion carrying one or more negative charges; Y and Y' are H, or an alkyl; R2 is NH 2 , NHBoc, or H; and Z is S or Se. R comprises a carbon-carbon double bond, carbon-oxygen double bond, carbon-sulfur double bond, carbon-nitrogen double bond, a carbon-carbon triple bond, carbon-nitrogen triple bond, an aromatic carbocyclic or heterocyclic system in β-position to the sulfonium center, unsaturated c-c bond, or c-heteroatom bond where the heteroatom is O, N, S.

Abstract translation: 公开了甲基转移酶的辅因子类似物。 化合物由式（I）表示，其中R 1是COOH或COO-; X是带有一个或多个负电荷的有机或无机阴离子; Y和Y'是H或烷基; R2是NH2，NHBOC或H; Z为S或Se。 R包括碳 - 碳双键，碳 - 氧双键，碳 - 硫双键，碳 - 氮双键，碳 - 碳三键，碳 - 氮三键，β位上的芳族碳环或杂环体系 到锍中心，不饱和cc键或c-杂原子键，其中杂原子是O，N，S。

公开(公告)号：WO2014160343A1

公开(公告)日：2014-10-02

申请号：PCT/US2014/026350

申请日：2014-03-13

Applicant: COSKATA, INC.

Inventor： REEVES, Andrew

IPC: C12N1/21 ,  C12N9/10 ,  C12N15/54 ,  C12N15/70

CPC classification number: C12N15/74 ,  C12N9/1007 ,  C12N15/63 ,  C12N15/70 ,  C12N15/87 ,  C12Q2521/331 ,  C12Y201/01072

Abstract: This invention provides isolated polynucleotides encoding DNA Type I methyltransferase and uses thereof for improving transformation efficiencies of exogenous and endogenous plasmid DNA into Clostridial hosts.

Abstract translation: 本发明提供编码DNA I型甲基转移酶的分离的多核苷酸及其用于提高外源和内源质粒DNA转化成梭菌宿主的转化效率的用途。

公开(公告)号：WO2011139994A2

公开(公告)日：2011-11-10

申请号：PCT/US2011/034852

申请日：2011-05-02

Applicant: BATTELLE ENERGY ALLIANCE, LLC ,  LEE, Brady, D. ,  NEWBY, Deborah, T. ,  LACEY, Jeffrey, A. ,  THOMPSON, David, N. ,  THOMPSON, Vicki, S. ,  APEL, William, A. ,  ROBERTO, Francisco, F. ,  REED, David, W.

Inventor： LEE, Brady, D. ,  NEWBY, Deborah, T. ,  LACEY, Jeffrey, A. ,  THOMPSON, David, N. ,  THOMPSON, Vicki, S. ,  APEL, William, A. ,  ROBERTO, Francisco, F. ,  REED, David, W.

IPC: C12N9/02 ,  C07H21/00 ,  C12N9/14 ,  C12N9/00 ,  C12N9/52 ,  C07K14/195 ,  C12N9/16 ,  C12N9/10 ,  C12N9/12 ,  C12N9/90

CPC classification number: C12N15/74 ,  C07K14/195 ,  C12N9/00 ,  C12N9/1007 ,  C12N9/52 ,  C12N15/87 ,  C12Y201/01072

Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius .

Abstract translation: 提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。 进一步提供的是使用分离和/或纯化的多肽和/或酸碱热杆菌的核酸序列调节或改变细胞内部或外部的重组的方法。

公开(公告)号：WO2015059690A1

公开(公告)日：2015-04-30

申请号：PCT/IL2014/050902

申请日：2014-10-14

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD.

Inventor： SOREK, Rotem ,  SBERRO, Hila ,  GOLDFARB, Tamara

IPC: C12N9/10 ,  C07K14/195

CPC classification number: C12N1/20 ,  A23C9/1234 ,  A23C2220/202 ,  C07K14/195 ,  C07K14/245 ,  C07K14/32 ,  C12N9/0051 ,  C12N9/1007 ,  C12N9/12 ,  C12N9/14 ,  C12N9/16 ,  C12N9/48 ,  C12N15/52 ,  C12Y108/04008 ,  C12Y201/01072 ,  C12Y207/11001 ,  C12Y301/03001 ,  C12Y304/00 ,  C12Y306/04012 ,  C12Y306/04013

Abstract: Isolated polynucleotides encoding a BREX system are provided. Accordingly there is provided an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ and at least one of pglX, pglXI, brxP, brxHI, brxHH, brxL, brxD, brxA, brxB, brxF , and brxE , with the proviso that said BREX system does not comprise pglW , and wherein said BREX system confers phage resistance to a bacteria recombinantiy expressing same; Also provided is an isolated polynucleotide encoding a BREX system comprising a nucleic acid sequence encoding the BREX system comprising brxC/pglY, pglZ, pglX, pglW and at least one of brxD and brxHI , and wherein said BREX system confers phage resistance to a bacteria recombinantiy expressing same. Also provided are compositions and methods for conferring phage resistance to bacteria or for conferring bacterial susceptibility to phages.

Abstract translation: 提供编码BREX系统的分离的多核苷酸。 因此，提供了编码BREX系统的分离的多核苷酸，其包含编码BREX系统的核酸序列，其包含brxC / pglY，pglZ和pglX，pglXI，brxP，brxHI，brxHH，brxL，brxD，brxA，brxB，brxF中的至少一种 和brxE，条件是所述BREX系统不包含pglW，并且其中所述BREX系统赋予对表达其的重组细菌的噬菌体抗性; 还提供了编码BREX系统的分离的多核苷酸，其包含编码包含brxC / pglY，pglZ，pglX，pglW和至少一种brxD和brxHI的BREX系统的核酸序列，并且其中所述BREX系统赋予对重组细菌的噬菌体抗性 表达相同。 还提供了赋予细菌噬菌体抗性或赋予细菌对噬菌体易感性的组合物和方法。

公开(公告)号：WO2011139994A3

公开(公告)日：2012-01-05

申请号：PCT/US2011034852

申请日：2011-05-02

Applicant: BATTELLE ENERGY ALLIANCE LLC ,  LEE BRADY D ,  NEWBY DEBORAH T ,  LACEY JEFFREY A ,  THOMPSON DAVID N ,  THOMPSON VICKI S ,  APEL WILLIAM A ,  ROBERTO FRANCISCO F ,  REED DAVID W

Inventor： LEE BRADY D ,  NEWBY DEBORAH T ,  LACEY JEFFREY A ,  THOMPSON DAVID N ,  THOMPSON VICKI S ,  APEL WILLIAM A ,  ROBERTO FRANCISCO F ,  REED DAVID W

IPC: C07H21/04

CPC classification number: C12N15/74 ,  C07K14/195 ,  C12N9/00 ,  C12N9/1007 ,  C12N9/52 ,  C12N15/87 ,  C12Y201/01072

Abstract: Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.

Abstract translation: 提供了分离和/或纯化的多肽和编码来自酸热环酸杆菌的多肽的核酸序列。 进一步提供的是使用分离和/或纯化的多肽和/或酸碱热杆菌的核酸序列调节或改变细胞内部或外部的重组的方法。

公开(公告)号：WO2007071962A1

公开(公告)日：2007-06-28

申请号：PCT/GB2006/004755

申请日：2006-12-19

Applicant: MEDICAL RESEARCH COUNCIL ,  MINCZUK, Michal ,  PAPWORTH, Monika, A. ,  KLUG, Aaron

Inventor： MINCZUK, Michal ,  PAPWORTH, Monika, A. ,  KLUG, Aaron

IPC: C12N15/11 ,  C12N15/79 ,  C07K19/00

CPC classification number: A61K38/1709 ,  A61K38/45 ,  A61K38/46 ,  C07K14/47 ,  C07K2319/07 ,  C07K2319/095 ,  C07K2319/81 ,  C12N9/1007 ,  C12N9/14 ,  C12Y201/01072 ,  C12Y306/03014

Abstract: Methods for delivering non-mitochondrial proteins to mitochondria are provided. Also provided are nucleic acid constructs comprising a coding sequence encoding a DNA- binding polypeptide, fused to a mitochondrial targeting sequence (MTS) and a nuclear export signal (NES), and the encoded proteins. The construct successfully delivers DNA binding proteins to the mitochondrion. A chimeric methylase based on the above construct is successfully delivered to mitochondria, resulting in modification of mtDNA.

Abstract translation: 提供了将线粒体蛋白递送至线粒体的方法。 还提供了包含编码与线粒体靶向序列（MTS）和核出口信号（NES）融合的DNA结合多肽的编码序列和编码的蛋白质的核酸构建体。 该构建体成功地将DNA结合蛋白递送至线粒体。 基于上述构建体的嵌合甲基化酶被成功地递送至线粒体，导致mtDNA的修饰。