公开(公告)号：WO2015175308A1

公开(公告)日：2015-11-19

申请号：PCT/US2015/029648

申请日：2015-05-07

Applicant: DANISCO US INC.

Inventor： BODO, Michael ,  COHEN, Jeffrey David ,  LIU, Chuanbin

IPC: C12P19/02 ,  C12P19/14

CPC classification number: C12P19/02 ,  C12P19/14

Abstract: Provided is an improved method or process for saccharifying a lignocellulosic biomass feedstock into fementable sugars, comprising the maintenance of dissolved oxygen levels in the saccharification mixture. Provided is also an improved apparatus or reactor useful for practicing the improved method or process.

Abstract translation: 提供了一种用于将木质纤维素生物质原料糖化为可发酵糖的改进方法或方法，其包括在糖化混合物中维持溶解氧水平。 还提供了一种用于实施改进的方法或方法的改进的装置或反应器。

公开(公告)号：WO2021092356A1

公开(公告)日：2021-05-14

申请号：PCT/US2020/059379

申请日：2020-11-06

Applicant: DANISCO US INC

Inventor： BODIE, Elizabeth A. ,  CHEN, Zhongqiang ,  LIU, Chuanbin ,  WARD, Michael

IPC: C12N15/90

Abstract: The present disclosure is generally related to modified filamentous fungal strains (cells) comprising enhanced protein productivity phenotypes, wherein such modified strains are particularly well-suited for growth in submerged cultures (e.g., large-scale production of proteins for industrial/commercial applications). Thus, certain embodiments of the disclosure are related to such variant (modified) strains of filamentous fungus derived (obtained) from parental strains comprising a gene encoding a native GEF1 protein, wherein the variant strain comprises a genetic modification which disrupts or deletes a gene encoding a native GEF1 protein, wherein the variant strain comprises an increased protein productivity phenotype (i.e., relative to the parental strain) when grown/cultivated/fermented under the same conditions.

公开(公告)号：WO2018106656A1

公开(公告)日：2018-06-14

申请号：PCT/US2017/064651

申请日：2017-12-05

Applicant: DANISCO US INC

Inventor： LAM, Anna, May ,  LIU, Chuanbin ,  TORRES PAZMINO, Daniel, Esteban ,  DOUGLAS, Nicholai, Rainsong ,  KIM, Steven, Sungjin ,  KAPER, Thijs ,  KARKEHABADI, Saeid ,  HANSSON, Henrik ,  SANDGREN, Mats

IPC: C12N9/02 ,  C12P19/04 ,  C12N9/42

CPC classification number: C12N9/0083 ,  C12N9/2437 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C12Y114/99

Abstract: Disclosed herein are truncated lytic polysaccharide mono-oxygenase (LPMO) enzymes lacking a carbohydrate-binding module (CBM). Further disclosed, for example, are compositions comprising these enzymes, and methods of producing and using them. In addition, combinations of truncated LPMOs and full-length LPMOs are disclosed to have improved cellulase-enhancing activities.