公开(公告)号：WO2015116395A1

公开(公告)日：2015-08-06

申请号：PCT/US2015/011527

申请日：2015-01-15

Applicant: DANISCO US INC.

Inventor： TEUNISSEN, Paula Johanna Maria ,  PEPSIN, Michael Jay ,  SHARMA, Vivek

IPC: C12N9/24 ,  C12P7/64

CPC classification number: C12N9/2482 ,  C11B1/025 ,  C11B13/00 ,  C12P7/06 ,  C12P7/64 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01008 ,  Y02E50/17 ,  Y02W30/74

Abstract: Provided are GH10 xylanases or a fragment thereof including modified xlyanase enzymes, all having xylanase activity, wherein the enzyme or fragment thereof provides increased oil recovery from a grain-based material compared with a control or parent GH10 xylanase enzyme, the parent GH10 xylanase having been modified at at least one or more of the following positions 7, 25, 33, 64, 79, 89, 217 and 298, wherein the numbering is based on the amino acid numbering of FveXyn4 (SEQ ID No. 1). Methods of using the GH10 xylanases, for example, for improved oil recovery are also provided.

Abstract translation: 提供的GH10木聚糖酶或其片段包括全部具有木聚糖酶活性的改性的xlyanase酶，其中与对照或亲本GH10木聚糖酶相比，其酶或片段提供了从谷物基材料提高的油回收率，母本GH10木聚糖酶已被 在以下至少一个或多个位置7,25,33,64,79,89,217和298处修饰，其中所述编号基于FveXyn4（SEQ ID No.1）的氨基酸编号。 还提供了使用GH10木聚糖酶的方法，例如用于改进的油回收。