公开(公告)号：WO9720067A2

公开(公告)日：1997-06-05

申请号：PCT/DE9602274

申请日：1996-11-27

Applicant: DEUTSCHES KREBSFORSCH ,  DEBATIN KLAUS MICHAEL ,  HERR INGRID

Inventor： DEBATIN KLAUS-MICHAEL ,  HERR INGRID

IPC: C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6851 ,  C12Q2600/158

Abstract: The invention relates to a process for quantitative determination of CD95 ligand, comprising the following steps: (a) isolation of total RNA from cells; (b) transcription of total RNA from (a) into cDNA by reverse transcription and; (c) amplification of cDNA from (b) and a CD95 ligand competitor fragment by CD95 ligand specific primers in a PCR reaction. The process is also suitable for determining the extent and/or course of apoptosis.

Abstract translation: 本发明涉及一种用于定量测定CD95配体的方法，包括以下步骤：（a）从细胞的全RNA的分离; （B）（a）的成通过逆转录的cDNA的总RNA的说明; 和（c）的cDNA的扩增（b）和通过在PCR反应中CD95配体特异性引物CD95配竞争者的片段。 该方法适合用于确定细胞凋亡的程度和/或过程。

公开(公告)号：WO03013591A3

公开(公告)日：2003-04-17

申请号：PCT/EP0208927

申请日：2002-08-09

Applicant: DEUTSCHES KREBSFORSCH ,  HERR INGRID ,  DEBATIN KLAUS-MICHAEL

Inventor： HERR INGRID ,  DEBATIN KLAUS-MICHAEL

IPC: A61K38/48 ,  A61P35/00 ,  C12Q1/68 ,  G01N33/50

CPC classification number: A61K38/4873

Abstract: Described are pharmaceutical compositions containing a caspase-8 and/or caspase-9 protein and/or (a) DNA sequence(s) encoding a caspase-8 and/or caspase-9 protein and a suitable pharmaceutical carrier. Said compositions are useful for restoring apoptosis sensitivity of glucorticoid-induced resistance of carcinoma cells towards cytotoxic therapy.

Abstract translation: 描述了含有半胱天冬酶-8和/或半胱天冬酶-9蛋白和/或（a）编码半胱天冬酶-8和/或半胱天冬酶-9蛋白的DNA序列和合适的药物载体的药物组合物。 所述组合物可用于恢复糖皮质激素诱导的癌细胞对细胞毒性治疗的耐药性的细胞凋亡敏感性。

公开(公告)号：WO9720064A3

公开(公告)日：1997-08-28

申请号：PCT/DE9602275

申请日：1996-11-27

Applicant: DEUTSCHES KREBSFORSCH ,  DEBATIN KLAUS MICHAEL ,  FRIESEN CLAUDIA ,  KRAMMER PETER ,  HERR INGRID

Inventor： DEBATIN KLAUS-MICHAEL ,  FRIESEN CLAUDIA ,  KRAMMER PETER ,  HERR INGRID

IPC: C12N15/09 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/50

CPC classification number: G01N33/5011

Abstract: The invention relates to a process for determining the activity, in particular cytostatic activity, of drugs. Said process comprises the following steps: (a) incubation of the drugs with cells, and; (b) detecting the extent of apoptosis which is caused by the CD95/CD95 ligand system.

公开(公告)号：WO0208450A3

公开(公告)日：2002-08-08

申请号：PCT/DE0102707

申请日：2001-07-24

Applicant: DEUTSCHES KREBSFORSCH ,  HERR INGRID ,  DEBATIN KLAUS-MICHAEL

Inventor： HERR INGRID ,  DEBATIN KLAUS-MICHAEL

IPC: C12Q1/48 ,  G01N33/68 ,  G01N33/573

CPC classification number: C12Q1/485 ,  G01N33/6872 ,  G01N2800/52

Abstract: The invention relates to a method for measuring the JNK/SAPK activity which is based on the implemation of a kinase reaction with JNK/SAPKs which are obtained from a cell lysate and preferably isolated by immune precipitation in the presence of ATP and GST transcription factors (preferably: GST-c-Jun), and subsequently measuring the amount of phosphorylate GST transcription factors (preferably: p-GST-c-Jun) according to the specific binding of an anti-p-GST transcription factor antibody(preferably: anti-p-GST-c-Jun-antibody).

Abstract translation: 公开的是用于确定JNK / SAPKs，这（与来自细胞裂解物获得的激酶反应的实施，优选通过免疫沉淀JNK分离的活性的方法/在ATP和GST-转录因子的存在下，优选SAPKs：GST-c-Jun的 ）和磷酸化的GST-转录因子的量的后续确定（优选的：通过特定的抗p-GST转录因子抗体的结合对GST-c-Jun的）（优选：抗p-GST-c-Jun的抗体 ）为基础的。