公开(公告)号：WO2016197123A3

公开(公告)日：2017-01-26

申请号：PCT/US2016036050

申请日：2016-06-06

Applicant: DOUGLAS SCIENT LLC

Inventor： HJELSETH ROBERT ,  HODGSON CORY ,  BRISTOW ANDREW ,  BAUMGARTNER MARK ,  SELLNOW DAVID ,  KEPPEL CASSIE ,  STECKELBERG JOHN ,  NEWMAN PAUL

IPC: B01L3/00 ,  C12M1/33 ,  C12M3/00 ,  G01N1/28

CPC classification number: B01L3/50 ,  B01L3/502 ,  B01L3/508 ,  B01L3/5635 ,  B01L2200/026 ,  B01L2200/0631 ,  B01L2300/021 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0832 ,  B01L2400/0457 ,  C12M45/06

Abstract: Embodiments of the invention provide a filter kit including filters for processing a biological sample. Some embodiments include a filter cap in a tube kit with a first tube containing a buffer solution and a second tube containing a lyophilized master mix. Some embodiments include a method of processing a sample using the kit including mixing a biological sample in a first tube with the buffer solution, positioning the filter cap in the first tube, positioning a second tube on the filter cap, flipping the first tube, the filter cap, and the second cap to filter the biological sample and buffer solution mixture with the filter cap as it flows from the first tube to the second tube. Some embodiments include structure enabling transfer of materials through inline flow between the tubes. Some further embodiments include integrated structure for sample pulverization with integrated buffer and lyophilized master mix.

Abstract translation: 本发明的实施方案提供了一种过滤器套件，其包括用于处理生物样品的过滤器。 一些实施例包括管套件中的过滤器盖，其中第一管含有缓冲溶液，第二管含有冻干的主混合物。 一些实施方案包括使用试剂盒处理样品的方法，包括将第一管中的生物样品与缓冲溶液混合，将过滤帽定位在第一管中，将第二管定位在过滤帽上，翻转第一管， 过滤器盖和第二盖，以便当过滤帽从第一管流向第二管时，过滤生物样品和缓冲溶液混合物与过滤嘴盖。 一些实施例包括能够通过管之间的直列流传输材料的结构。 一些另外的实施方案包括用于具有集成缓冲剂和冻干的主混合物的样品粉碎的整体结构。