公开(公告)号：WO2013164479A3

公开(公告)日：2013-12-19

申请号：PCT/EP2013059315

申请日：2013-05-03

Applicant: DSM IP ASSETS BV

Inventor： DEKKER PETRUS JACOBUS THEODORUS ,  JONG DE RENE MARCEL ,  MUIJLWIJK CORNELIS MARINUS

IPC: C12N9/64 ,  A23C19/04 ,  C12N1/19 ,  C12N15/59

CPC classification number: C12N9/6483 ,  A23C19/032 ,  A23C19/0326 ,  A23C19/0684 ,  A23C19/072 ,  A23C2220/202 ,  A23C2250/15 ,  C12Y304/23004

Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.

Abstract translation: 具有凝乳酶活性的变体多肽，其中所述变体具有当与包含SEQ ID NO：2所示的序列的凝乳酶比对时的氨基酸序列包含对应于任何氨基酸的氨基酸残基的至少一个取代 2，22，40，48，50，51，53，61，62，76，88，98，99，109，112，117，125，126，135，144，160，161，163，187， 194,2200,201,202,203,221,223,240,242,244,254,267,271,273,278,280,284,289,292,294或295表示所述位置是参照SEQ ID NO：2，其中与具有凝乳酶活性的参照多肽相比，变体具有一个或多个改变的特性。 这种变体多肽可用于制备奶酪。

公开(公告)号：WO2012049179A2

公开(公告)日：2012-04-19

申请号：PCT/EP2011067738

申请日：2011-10-11

Applicant: DSM IP ASSETS BV ,  WISSELINK HENDRIK WOUTER ,  MARIS VAN ANTONIUS JEROEN ADRIAAN ,  PRONK JACOBUS THOMAS ,  KLAASSEN PAUL ,  JONG DE RENE MARCEL

Inventor： WISSELINK HENDRIK WOUTER ,  MARIS VAN ANTONIUS JEROEN ADRIAAN ,  PRONK JACOBUS THOMAS ,  KLAASSEN PAUL ,  JONG DE RENE MARCEL

IPC: C12N1/18 ,  C12N9/12 ,  C12P7/08

CPC classification number: C12N15/81 ,  C07K14/395 ,  C12N9/1205 ,  C12P7/06 ,  C12P7/10 ,  Y02E50/16 ,  Y02E50/17

Abstract: The invention relates to a polypeptide having a mutation at one or more position corresponding to Y274 and/or D376 of SEQ ID NO: 51, wherein the polypeptide has at least 50% sequence identity with SEQ ID NO: 51, and wherein the polypeptide has kinase activity.

Abstract translation: 本发明涉及在一个或多个对应于SEQ ID NO：51的Y274和/或D376的位置具有突变的多肽，其中所述多肽与SEQ ID NO：51具有至少50％的序列同一性，并且其中所述多肽具有 激酶活性。