IMPROVED CHYMOSINE ENZYME VARIANTS
    1.
    发明申请
    IMPROVED CHYMOSINE ENZYME VARIANTS 审中-公开
    改善CHYMOSINE酶变异

    公开(公告)号:WO2013164479A3

    公开(公告)日:2013-12-19

    申请号:PCT/EP2013059315

    申请日:2013-05-03

    Abstract: A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.

    Abstract translation: 具有凝乳酶活性的变体多肽,其中所述变体具有当与包含SEQ ID NO:2所示的序列的凝乳酶比对时的氨基酸序列包含对应于任何氨基酸的氨基酸残基的至少一个取代 2,22,40,48,50,51,53,61,62,76,88,98,99,109,112,117,125,126,135,144,160,161,163,187, 194,2200,201,202,203,221,223,240,242,244,254,267,271,273,278,280,284,289,292,294或295表示所述位置是参照SEQ ID NO:2,其中与具有凝乳酶活性的参照多肽相比,变体具有一个或多个改变的特性。 这种变体多肽可用于制备奶酪。

    METHOD FOR MAKING CHEESE
    2.
    发明申请
    METHOD FOR MAKING CHEESE 审中-公开
    制作方法

    公开(公告)号:WO2013093049A3

    公开(公告)日:2013-08-15

    申请号:PCT/EP2012076744

    申请日:2012-12-21

    Applicant: CHR HANSEN AS

    Abstract: The present invention relates to a method for producing cheese with good flavour, especially low-salt cheese by using a lactic acid bacteria (LAB) starter culture which is glutamate decarboxylation (GAD) negative. The method may additionally involve inoculating the milk substrate with a GAD-negative Lb. helveticus strain having a high lytic and peptidolytic activity, which further releases glutamate into the milk. Additional application of camel chymosin reduces bitterness.

    Abstract translation: 本发明涉及通过使用谷氨酸脱羧(GAD)阴性的乳酸菌(LAB)起始培养物,生产具有良好风味的干酪,特别是低盐干酪的方法。 该方法还可以包括用GAD阴性Lb接种乳底物。 具有高裂解活性和解肽活性的螺旋藻菌株,其进一步将谷氨酸释放到牛奶中。 骆驼凝乳酶的额外应用可减少苦味。

    METHOD FOR HIGH-THROUGHPUT CHEESE MAKING
    7.
    发明申请
    METHOD FOR HIGH-THROUGHPUT CHEESE MAKING 审中-公开
    高通量制作方法

    公开(公告)号:WO2008153387A1

    公开(公告)日:2008-12-18

    申请号:PCT/NL2008/050369

    申请日:2008-06-12

    CPC classification number: A23C19/072 A23C19/0688

    Abstract: The present invention relates to a method for producing cheese. The method involves very small quantities of milk ranging between 0.1 ml and 160 ml, and is particularly useful in high - throughput cheese making applications. Cheese is produced in at least two vats. In a particularly favourable embodiment, each of the 96 wells of a commercially available microtitre plate is employed as a cheese vat, allowing for the production of 96 cheeses in one run. The cheeses thus produced have been analysed in terms of their flavour profiIe, using high - throughput screening techniques such as GC - MS.

    Abstract translation: 本发明涉及一种生产奶酪的方法。 该方法涉及0.1ml至160ml之间的非常少量的牛奶,并且特别适用于高通量奶酪制备应用。 奶酪至少在两个桶内生产。 在特别有利的实施方案中,将市售的微量滴定板的96个孔中的每一个用作奶酪桶,从而允许在一次运行中生产96个奶酪。 已经使用高通量筛选技术如GC-MS对其生产的奶酪进行了风味分析。

Patent Agency Ranking