Abstract:
A variant polypeptide having chymosin activity, wherein the variant has an amino acid sequence which, when aligned with the chymosin comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue corresponding to any of amino acids 2, 22, 40, 48, 50, 51, 53, 61, 62, 76, 88, 98, 99, 109, 112, 117, 125, 126, 135, 144, 160, 161, 163, 187, 189, 194, 200, 201, 202, 203, 221, 223, 240, 242, 244, 254, 267, 271, 273, 278, 280, 284, 289, 292, 294 or 295 said positions being defined with reference to SEQ ID NO: 2 and wherein the variant has one or more altered properties as compared with a reference polypeptide having chymosin activity. Such a variant polypeptide may be used in the preparation of a cheese.
Abstract translation:具有凝乳酶活性的变体多肽,其中所述变体具有当与包含SEQ ID NO:2所示的序列的凝乳酶比对时的氨基酸序列包含对应于任何氨基酸的氨基酸残基的至少一个取代 2,22,40,48,50,51,53,61,62,76,88,98,99,109,112,117,125,126,135,144,160,161,163,187, 194,2200,201,202,203,221,223,240,242,244,254,267,271,273,278,280,284,289,292,294或295表示所述位置是参照SEQ ID NO:2,其中与具有凝乳酶活性的参照多肽相比,变体具有一个或多个改变的特性。 这种变体多肽可用于制备奶酪。
Abstract:
The present invention relates to a method for producing cheese with good flavour, especially low-salt cheese by using a lactic acid bacteria (LAB) starter culture which is glutamate decarboxylation (GAD) negative. The method may additionally involve inoculating the milk substrate with a GAD-negative Lb. helveticus strain having a high lytic and peptidolytic activity, which further releases glutamate into the milk. Additional application of camel chymosin reduces bitterness.
Abstract:
Disclosed herein is the use of crosslinked beta-cyclodextrin as a trap for removing cholesterol. The crosslinked beta-cyclodextrin is prepared by crosslinking beta-cyclodextrin in the presence of a crosslinking agent. Treatment with the crosslinked beta-cyclodextrin results in cholesterol-depleted foods. After application to foods, the crosslinked beta-cyclodextrin which traps cholesterol therein can be readily regenerated with organic solvents. The crosslinked beta-cyclodextrin can be applied to almost all cholesterol-containing foods, such as dairy products, meat products, and egg products, with excellent cholesterol removal rates.
Abstract:
The present invention relates to a food product which food product is frozen and thawed before consumption comprising AFP19 as well as to methods for preparing such a food product by incorporating AFP19. The invention further describes production of an ice structuring protein (ISP), specifically, the ice structuring protein from Leucosporidium sp (AFP19) in high amounts in filamentous fungi such as Aspergillus niger or Aspergillus oryzae.
Abstract:
The present invention relates to an aerated cheese product. The present invention further relates to methods of preparing said aerated cheese product and to the aerated cheese product obtainable by the methods.
Abstract:
Methods as can be used in the preparation of one or more dairy, dairy analog and cheese products from a range of proteinacous starting materials, such methods comprising protein modification and protein restoration or protein restructure.
Abstract:
The present invention relates to a method for producing cheese. The method involves very small quantities of milk ranging between 0.1 ml and 160 ml, and is particularly useful in high - throughput cheese making applications. Cheese is produced in at least two vats. In a particularly favourable embodiment, each of the 96 wells of a commercially available microtitre plate is employed as a cheese vat, allowing for the production of 96 cheeses in one run. The cheeses thus produced have been analysed in terms of their flavour profiIe, using high - throughput screening techniques such as GC - MS.
Abstract:
The present invention relates to methods for coagulating bovine milkand making cheese using a coagulant substantially identical to a chymosin originating from an animal of the Tylopoda suborder.
Abstract:
The invention relates to the field of food production methods, whereby at least two micro-organisms are used, preferably a yeast and a bacterial strain.
Abstract:
The present invention relates to a composition comprising a coupled enzyme system for the rapid and efficient production of hydrogen peroxide by the coupling of a first enzyme system capable of hydrogen peroxide generation, to a second enzyme system which utilizes the non hydrogen peroxide product of the first enzyme system, and optionally is capable of generating further hydrogen peroxide.