公开(公告)号：WO2021219777A1

公开(公告)日：2021-11-04

申请号：PCT/EP2021/061244

申请日：2021-04-29

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： JEDRZEJOWSKI, Anäis ,  FREMAUX, Christophe ,  GUILLOU, Elodie ,  VAN DILLEN, Sabine ,  HORVATH, Philippe ,  PIANFETTI, Mikael

IPC分类号： C12R1/46 ,  C07K14/315 ,  C12N15/00

摘要： The invention relates to a method for generating a Streptococcus thermophilus strain exhibiting a "high galactose utilization" profile, which is characterized by a high percentage of consumed galactose at the maximum speed of lactose consumption and optionally a high percentage of consumed galactose at the end of lactose consumption (both as determined by assay I), comprising modifying the sequence of the gal-lac gene cluster. The invention also relates to a Streptococcus thermophilus strain obtained or obtainable by this method, to a Streptococcus thermophilus strain characterized both by the sequence of its gal-lac gene cluster and its "high galactose utilization" profile, to a culture and kit-of part comprising such strains, as well as to the use of such strains in food applications.

公开(公告)号：WO2020182976A1

公开(公告)日：2020-09-17

申请号：PCT/EP2020/056766

申请日：2020-03-13

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： JEDRZEJOWSKI, Anäis ,  FREMAUX, Christophe ,  VAN DILLEN, Sabine ,  DESFOUGÈRES, Thomas ,  JODEAU, Max Charles ,  LUGAND, Damien

IPC分类号： C12R1/46 ,  C12P7/56 ,  A23C9/12 ,  C12N9/38

摘要： The invention relates to a polynucleotide comprising a lacZ gene ( lacZ FS ) encoding a β-galactosidase characterized by a particular profile regarding its efficiency of hydrolysis of lactose. The invention is also directed to a Streptococcus thermophilus strain comprising a lacZ FS allele and bacterial composition thereof, and their use to obtain fermented milk not undergoing post-acidification.