公开(公告)号：WO2008067807A3

公开(公告)日：2008-09-12

申请号：PCT/DE2007002216

申请日：2007-12-10

Applicant: EVOCATAL GMBH ,  EGGERT THORSTEN ,  ANDEXER JENNIFER

Inventor： EGGERT THORSTEN ,  ANDEXER JENNIFER

IPC: C12N9/88 ,  C12N5/10 ,  C12P13/00

CPC classification number: C12N9/88 ,  C12P13/004

Abstract: The invention relates to a polypeptide which can be isolated from plants of the Brassicaceae family and which has at least the activity of a hydroxynitrile lyase (HNL). Said hydroxynitrile lyase is the first HNL from the Brassicaceae family. Furthermore, the plants (Arabidopsis) from which said enzyme or the gene therefor are isolated are not described as cyanogens. All HNL containing plants described up to now are cyanogenic plants, so it has been assumed up to now that only cyanogenic plants contain hydroxynitrile lyases. Surprisingly it has been found that such a polypeptide (AtHNL) is (R) selective. From the amino acid sequence a theoretical molecular weight of 29,2 kDa is found for the AtHNL sub-unit. The calculated molecular mass of a protein of ca. 30 kDa can be confirmed using SDS gel electrophoresis.

Abstract translation: 本发明涉及可以从十字花科植物中分离并且至少具有羟基腈裂解酶（HNL）活性的多肽。 根据本发明的羟腈裂解酶是来自十字花科的第一种HNL。 该酶或其基因被分离的植物（拟南芥）也被描述为非氰。 迄今为止描述的所有含有HNL的植物都是含氰的植物，因此之前假定只有含氰的植物含有羟基腈裂解酶。 令人惊讶的是，已经发现根据本发明的多肽（AtHNL）（R）是选择性的。 从氨基酸序列得到AtHNL亚基的理论分子量为29.2kDa。 约30kDa蛋白质的计算分子量可以通过SDS凝胶电泳确认。