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    PROCESS FOR PRODUCING THIOESTERS EMPLOYING A SORTASE A
    3.
    发明申请
    PROCESS FOR PRODUCING THIOESTERS EMPLOYING A SORTASE A 审中-公开
    生产使用SORTASE的硫酸酯的方法A

    公开(公告)号：WO2017050874A1

    公开(公告)日：2017-03-30

    申请号：PCT/EP2016/072512

    申请日：2016-09-22

    Applicant: F. HOFFMANN-LA ROCHE AG ,  HOFFMANN-LA ROCHE INC.

    Inventor： SCHATTE, Martin ,  BOENITZ-DULAT, Mara ,  BERGMANN, Frank

    IPC: C12P11/00 ,  C12P21/02

    CPC classification number: C12P11/00 ,  A61K47/65 ,  C12P21/02

    Abstract: Herein is reported a method for the enzymatic production of a thioester comprising incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue), ii) a second polypeptide that has at its N-terminus a cysteine amino acid residue or is a cysteinyl compound, and iii) a third polypeptide with sortase A activity.

    Abstract translation: 本文报道了一种用于酶促生产硫酯的方法，包括孵育i）包含氨基酸序列LPXTG（SEQ ID NO：01，其中X可以是任何氨基酸残基）的第一多肽，ii）具有 其N末端是半胱氨酸氨基酸残基或是半胱氨酰化合物，和iii）具有分选酶A活性的第三种多肽。

    IMPROVED SORTASE
    6.
    发明申请
    IMPROVED SORTASE 审中-公开
    改进的SORTASE

    公开(公告)号：WO2017167712A1

    公开(公告)日：2017-10-05

    申请号：PCT/EP2017/057247

    申请日：2017-03-28

    Applicant: F. HOFFMANN-LA ROCHE AG ,  HOFFMANN-LA ROCHE INC.

    Inventor： BOENITZ-DULAT, Mara ,  SCHATTE, Martin

    IPC: C12N9/52 ,  C12N15/57

    Abstract: Reported herein is a sortase comprising an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 11 and that comprises the mutations D101S and K137S.

    Abstract translation: 本文报道了分选酶，其包含与SEQ ID NO：11的氨基酸序列至少90％相同并且包含突变D101S和K137S的氨基酸序列。

    TRANSAMIDATION EMPLOYING SORTASE A IN DEEP EUTECTIC SOLVENTS
    7.
    发明申请
    TRANSAMIDATION EMPLOYING SORTASE A IN DEEP EUTECTIC SOLVENTS 审中-公开
    在深层保护溶剂中使用SORTASE A的活化剂

    公开(公告)号：WO2017050872A1

    公开(公告)日：2017-03-30

    申请号：PCT/EP2016/072510

    申请日：2016-09-22

    Applicant: F. HOFFMANN-LA ROCHE AG ,  HOFFMANN-LA ROCHE INC.

    Inventor： SCHATTE, Martin ,  BOENITZ-DULAT, Mara

    IPC: C12P21/02

    CPC classification number: C12P21/02

    Abstract: Herein is reported a method for the enzymatic production of a polypeptide comprising the step of incubating i) a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 01, wherein X can be any amino acid residue) or LPXTA (SEQ ID NO: 41, wherein X can be any amino acid residue), ii) a second polypeptide that has i) a glycinyl, an alaninyl, or a cysteinyl compound at its N- terminus, or ii) an oligoglycine, or oligoalanine, or a cysteine amino acid residue followed by one to three glycine or alanine amino acid residues at its N-terminus, or iii) a lysine amino acid residue within its 5 N-terminal amino acid residues, and iii) a third polypeptide with sortase A activity, in a deep eutectic solvent and thereby producing a polypeptide.

    Abstract translation: 本文报道了一种用于酶产生多肽的方法，其包括以下步骤：i）包含氨基酸序列LPXTG（SEQ ID NO：01，其中X可以是任何氨基酸残基）或LPXTA（SEQ ID NO： ：41，其中X可以是任何氨基酸残基），ii）第二多肽，其i）在其N-末端具有甘氨酰基，丙烯酰基或半胱氨酰化合物，或ii）低聚赖氨酸或寡聚丙氨酸或半胱氨酸 氨基酸残基，其N-末端具有一至三个甘氨酸或丙氨酸氨基酸残基，或iii）其5个N-末端氨基酸残基内的赖氨酸氨基酸残基，和iii）具有分选酶A活性的第三多肽 深层共晶溶剂，从而产生多肽。

    NOVEL METHODS FOR ENZYME MEDIATED POLYPEPTIDE CONJUGATION USING SORTASE
    8.
    发明申请
    NOVEL METHODS FOR ENZYME MEDIATED POLYPEPTIDE CONJUGATION USING SORTASE 审中-公开
    使用SORTASE的酶介导多肽结合的新方法

    公开(公告)号：WO2016096741A1

    公开(公告)日：2016-06-23

    申请号：PCT/EP2015/079617

    申请日：2015-12-14

    Applicant: F. HOFFMANN-LA ROCHE AG ,  HOFFMANN-LA ROCHE INC.

    Inventor： BOENITZ-DULAT, Mara ,  KRATZSCH, Peter ,  SCHATTE, Martin

    IPC: C07K16/00 ,  C12N9/52 ,  C07K16/46 ,  C07K16/32

    CPC classification number: C12Y304/2207 ,  C07K16/00 ,  C07K16/32 ,  C07K16/46 ,  C07K2317/52 ,  C12N9/52 ,  C12N9/6472

    Abstract: Herein is reported a method for producing an enzymatic conjugation product of two polypeptides comprising incubating of a first polypeptide comprising the amino acid sequence LPXTG (SEQ ID NO: 20, wherein X can be any amino acid residue), a second polypeptide has an oligo-alanine A m (m = 2 (SEQ ID NO: 26), or 3 (SEQ ID NO: 27), or 4 (SEQ ID NO: 28), or 5 (SEQ ID NO: 29)) amino acid sequence at its N-terminus, a third polypeptide with sortase activity which is derived from Staphylococcus aureus Sortase A, and recovering the conjugate from the reaction mixture and thereby producing the enzymatic conjugation product of two polypeptides.

    Abstract translation: 本文报道了用于产生两种多肽的酶偶联产物的方法，包括孵育包含氨基酸序列LPXTG（SEQ ID NO：20，其中X可以是任何氨基酸残基）的第一多肽，第二多肽具有寡核苷酸， 丙氨酸Am（m = 2（SEQ ID NO：26）或3（SEQ ID NO：27）或4（SEQ ID NO：28）或5（SEQ ID NO：29））氨基酸序列 - 末端，具有来自金黄色葡萄球菌分选酶A的分选酶活性的第三种多肽，并从反应混合物中回收结合物，从而产生两种多肽的酶联结产物。

    ACTIVITY ASSAY FOR BOND FORMING ENZYMES
    9.
    发明申请
    ACTIVITY ASSAY FOR BOND FORMING ENZYMES 审中-公开
    用于粘结形成酶的活性测定

    公开(公告)号：WO2016096740A1

    公开(公告)日：2016-06-23

    申请号：PCT/EP2015/079615

    申请日：2015-12-14

    Applicant: F. HOFFMANN-LA ROCHE AG ,  HOFFMANN-LA ROCHE INC.

    Inventor： BOENITZ-DULAT, Mara ,  KOPETZKI, Erhard ,  KRATZSCH, Peter ,  SCHATTE, Martin

    IPC: C12Q1/37

    CPC classification number: G01N33/5302 ,  A61K38/00 ,  C12Q1/005 ,  C12Q1/37 ,  G01N2333/90 ,  G01N2333/96413

    Abstract: Herein is reported a method for the detection of a sortase in a sample, comprising the following steps: a) incubating the sample with a first substrate comprising an immobilization tag and a second substrate comprising a detectable label, whereby in the presence of a sortase in the sample a conjugate comprising the immobilization tag and the detectable label is formed, b) immobilizing the conjugate of step a) via/using the immobilization tag to a solid phase, c) detecting the immobilized conjugate via/using the detectable label and thereby detecting the sortase in the sample.

    Abstract translation: 本文报道了用于检测样品中分选酶的方法，包括以下步骤：a）将样品与包含固定标签的第一底物和包含可检测标记的第二底物孵育，由此在存在分选酶的情况下 形成包含固定标签和可检测标记物的缀合物，b）通过/使用固定标签固定步骤a）的缀合物至固相，c）通过/使用可检测标记物检测固定的缀合物，从而检测 样品中的分选酶。

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