公开(公告)号：WO2004003194A2

公开(公告)日：2004-01-08

申请号：PCT/EP2003/006711

申请日：2003-06-25

Applicant: FLANDERS INTERUNIVERSITY INSTITUTE FOR BIOTECHNOLOGY

Inventor： CONTRERAS, Roland ,  CALLEWAERT, Nico, L., M. ,  GEYSENS, Steven, C., J. ,  KAIGORODOV, Vladimir ,  WOUTER, Vervecken

IPC: C12N9/24

CPC classification number: C12Y302/01114 ,  C12N9/1288 ,  C12N9/2488 ,  C12N15/815 ,  C12Y302/01024

Abstract: The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the contemplated vectors are also included in this invention.

Abstract translation: 本发明提供了遗传工程改造的甲基营养酵母菌株，包括毕赤酵母，特别是巴斯德毕赤酵母能够产生具有降低或修饰的糖基化的蛋白质。 还提供了使用这种遗传工程改造的毕赤酵母菌株生产具有还原和/或修饰的糖基化的糖蛋白的方法。 包含用于转化甲基营养酵母的α-1,2-甘露糖苷酶I，葡糖苷酶II，GlcNAc转移酶I和甘露糖苷酶II或包含OCH1破坏序列的编码序列的载体被本发明考虑。 用于提供预期载体的试剂盒也包括在本发明中。