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1.发明申请
公开(公告)号：WO2011061629A2
公开(公告)日：2011-05-26
申请号：PCT/IB2010/003154
申请日：2010-11-19
Applicant: OXYRANE UK LIMITED , GEYSENS, Steven Christian, Jozef , VERVECKEN, Wouter
Inventor： GEYSENS, Steven Christian, Jozef , VERVECKEN, Wouter
IPC: C12N1/00
CPC classification number: C12N9/1051 , C07K16/32 , C07K2317/14 , C07K2317/41 , C12N9/2402 , C12N9/2488 , C12N9/60 , C12N15/81 , C12P21/005 , C12Y204/01 , C12Y204/01133 , C12Y302/01024 , C12Y302/01084 , C12Y302/01114
Abstract: Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosylation pathway.
Abstract translation: 本文描述了可用于产生含有哺乳动物样复合N-聚糖的靶分子或在哺乳动物糖基化途径中含有中间体的遗传工程真菌细胞。
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公开(公告)号：WO2007112757A3
公开(公告)日：2008-02-28
申请号：PCT/DK2007000177
申请日：2007-04-04
Applicant: ZYMENEX AS , NILSSON STEFAN
Inventor： NILSSON STEFAN
CPC classification number: A61K38/45 , A61K38/465 , A61K38/47 , C07K1/34 , C07K1/36 , C12N9/1085 , C12Y205/01061 , C12Y301/06001 , C12Y301/06008 , C12Y302/01024 , C12Y302/01046
Abstract: The present invention comprises a method of concentrating a composition comprising a polypeptide of interest and the use of such a concentrated composition for the treatment of diseases in mammals, in particular by subcutaneous injection.
Abstract translation: 本发明包括浓缩包含感兴趣的多肽的组合物和使用这种浓缩组合物用于治疗哺乳动物疾病，特别是通过皮下注射的方法。
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3.发明申请EXPRESSION OF CLASS 2 MANNOSIDASE AND CLASS III MANNOSIDASE IN LOWER EUKARYOTIC CELLS 审中-公开在较低的原核细胞中表达2类曼卡塞菌素和第3类芒果苷
公开(公告)号：WO2004074498A3
公开(公告)日：2005-06-23
申请号：PCT/US2004005132
申请日：2004-02-20
Applicant: HAMILTON STEPHEN R , GERNGROSS TILLMAN U , WILDT STEFAN , CHOI BYUNG-KWON , NETT JUERGEN HERMANN , BOBROWICZ PIOTR , DAVIDSON ROBERT C
Inventor： HAMILTON STEPHEN R , GERNGROSS TILLMAN U , WILDT STEFAN , CHOI BYUNG-KWON , NETT JUERGEN HERMANN , BOBROWICZ PIOTR , DAVIDSON ROBERT C
CPC classification number: C12P21/005 , A01K2217/075 , C12N9/1051 , C12N9/2488 , C12N15/79 , C12Y302/01024 , C12Y302/01114
Abstract: A method for producing human-like glycoproteins by expressing a Class 2 alpha-mannosidase having a substrate specificity for Manalphal,3 and Manalphal,6 glycosidic linkages in a lower eukaryote is disclosed. Hydrolysis of these linkages on oligosaccharides produces substrates for further N-glycan processing in the secretory pathway.
Abstract translation: 公开了通过在低等真核生物中表达具有Manalphal，3和Manalphal，6个糖苷键的底物特异性的2类α-甘露糖苷酶来生产人样糖蛋白的方法。 在寡糖上的这些键合的水解产生用于在分泌途径中进一步N-聚糖加工的底物。
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公开(公告)号：WO2004003194A2
公开(公告)日：2004-01-08
申请号：PCT/EP2003/006711
申请日：2003-06-25
Inventor： CONTRERAS, Roland , CALLEWAERT, Nico, L., M. , GEYSENS, Steven, C., J. , KAIGORODOV, Vladimir , WOUTER, Vervecken
IPC: C12N9/24
CPC classification number: C12Y302/01114 , C12N9/1288 , C12N9/2488 , C12N15/815 , C12Y302/01024
Abstract: The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the contemplated vectors are also included in this invention.
Abstract translation: 本发明提供了遗传工程改造的甲基营养酵母菌株，包括毕赤酵母，特别是巴斯德毕赤酵母能够产生具有降低或修饰的糖基化的蛋白质。 还提供了使用这种遗传工程改造的毕赤酵母菌株生产具有还原和/或修饰的糖基化的糖蛋白的方法。 包含用于转化甲基营养酵母的α-1,2-甘露糖苷酶I，葡糖苷酶II，GlcNAc转移酶I和甘露糖苷酶II或包含OCH1破坏序列的编码序列的载体被本发明考虑。 用于提供预期载体的试剂盒也包括在本发明中。
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公开(公告)号：WO1997010353A1
公开(公告)日：1997-03-20
申请号：PCT/US1996014730
申请日：1996-09-13
Inventor： VIRGINIA TECH INTELLECTUAL PROPERTY, INC. , CROPTECH DEVELOPMENT CORPORATION , RADIN, David, N. , CRAMER, Carole, L. , OISHI, Karen, K. , WEISSENBORN, Deborah, L.
IPC: C12P21/06
CPC classification number: C12Y302/01045 , A61K38/00 , C12N9/2402 , C12N15/8237 , C12N15/8257 , C12Y302/01018 , C12Y302/01022 , C12Y302/01024 , C12Y302/01049 , C12Y302/01076
Abstract: The invention relates to the production of enzymatically active recombinant human and animal lysosomal enzymes involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal lysosomal enzymes in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting enzymatic activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human hGC and IDUA nucleotide sequences produces enzymatically active modified human glucocerebrosidase and human alpha -L-iduronidase. The recombinant lysosomal enzymes produced in accordance with the invention may be used for a variety of purposes, including but not limited to enzyme replacement therapy for the therapeutic treatment of human and animal lysosomal storage diseases.
Abstract translation: 本发明涉及酶活性重组人和动物溶酶体酶的生产，其涉及重组表达构建体的构建和表达，其包含在植物表达系统中编码人或动物溶酶体酶的序列。 植物表达系统提供翻译后修饰和加工以产生显示酶活性的重组基因产物。 本发明通过其中具有包含人hGC和IDUA核苷酸序列的重组表达构建体的转基因烟草植物产生酶促活性修饰的人类葡糖脑苷脂酶和人α-L-胆碱苷酶的实施例来证明。 根据本发明生产的重组溶酶体酶可用于多种目的，包括但不限于用于治疗性治疗人和动物溶酶体贮积病的酶替代疗法。
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Patent Agency Ranking6.发明申请MANNOSIDASES CAPABLE OF UNCAPPING MANNOSE-1-PHOSPHO-6-MANNOSE LINKAGES AND DEMANNOSYLATING PHOSPHORYLATED N-GLYCANS AND METHODS OF FACILITATING MAMMALIAN CELLULAR UPTAKE OF GLYCOPROTEINS 审中-公开能够去除甘露糖-1-磷酸-6-甘露糖键和去甘氨酸化磷酸化N-甘露糖的甘露糖苷酶以及促进哺乳动物哺乳动物细胞摄取的方法
公开(公告)号：WO2012042386A3
公开(公告)日：2012-05-31
申请号：PCT/IB2011002770
申请日：2011-09-29
Applicant: OXYRANE UK LTD , NOELLA GWENDA PYNAERT , PIENS KATHLEEN CAMILLA TELESPHORE ALIDA MARIA , VALEVSKA ALBENA VERGILIEVA , VERVECKEN WOUTER
Inventor： NOELLA GWENDA PYNAERT , PIENS KATHLEEN CAMILLA TELESPHORE ALIDA MARIA , VALEVSKA ALBENA VERGILIEVA , VERVECKEN WOUTER
CPC classification number: C12P21/005 , A61K38/00 , A61K38/16 , A61K38/1709 , C12N9/2402 , C12N9/2488 , C12Y302/01 , C12Y302/01024 , Y02P20/52
Abstract: The invention provides mannosidases capable of uncapping mannose-l-phospho- 6-mannose moieties and demannosylating phosphorylated N-glycans, methods of using such mannosidases, glycoproteins produced using the methods, as well as methods of facilitating mammalian cellular uptake of glycoproteins.
Abstract translation: 本发明提供了能够脱帽甘露糖-1-磷酸-6-甘露糖部分和去甘露糖基化磷酸化N-聚糖的甘露糖苷酶，使用此类甘露糖苷酶的方法，使用所述方法产生的糖蛋白以及促进哺乳动物细胞摄取糖蛋白的方法。
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公开(公告)号：WO2011061629A3
公开(公告)日：2011-12-01
申请号：PCT/IB2010003154
申请日：2010-11-19
Applicant: OXYRANE UK LTD , GEYSENS STEVEN CHRISTIAN JOZEF , VERVECKEN WOUTER
Inventor： GEYSENS STEVEN CHRISTIAN JOZEF , VERVECKEN WOUTER
CPC classification number: C12N9/1051 , C07K16/32 , C07K2317/14 , C07K2317/41 , C12N9/2402 , C12N9/2488 , C12N9/60 , C12N15/81 , C12P21/005 , C12Y204/01 , C12Y204/01133 , C12Y302/01024 , C12Y302/01084 , C12Y302/01114
Abstract: Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosylation pathway.
Abstract translation: 本文描述了用于在哺乳动物糖基化途径中产生包含哺乳动物样复合体N-聚糖或含有中间体的靶分子的方法和基因工程真菌细胞。
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8.发明申请FUSION CONSTRUCTS AND USE OF SAME TO PRODUCE ANTIBODIES WITH INCREASED FC RECEPTOR BINDING AFFINITY AND EFFECTOR FUNCTION 审中-公开融合结构及其用途生产具有增强的FC受体结合亲和力和效应功能的抗体
公开(公告)号：WO2004065540A3
公开(公告)日：2005-03-24
申请号：PCT/IB2004000844
申请日：2004-01-22
Applicant: GLYCART BIOTECHNOLOGY AG , UMANA PABLO , BRUENKER PETER , FERRARA CLAUDIA , SUTER TOBIAS
Inventor： UMANA PABLO , BRUENKER PETER , FERRARA CLAUDIA , SUTER TOBIAS
IPC: A61K38/00 , C07K16/28 , C07K16/30 , C07K16/32 , C12N9/10 , C12N9/24 , C12N15/62 , C12P21/00 , C07K16/00 , C12N15/54
CPC classification number: C07K16/2887 , A61K38/00 , A61K2039/505 , C07K16/28 , C07K16/2863 , C07K16/2896 , C07K16/30 , C07K16/32 , C07K2317/24 , C07K2317/41 , C07K2317/732 , C07K2319/05 , C12N9/1051 , C12N9/2488 , C12N15/62 , C12P21/005 , C12Y204/01038 , C12Y204/01144 , C12Y302/01024 , C12Y302/01096 , C12Y302/01114
Abstract: The present invention relates to the field of glycosylation engineering of proteins. More particularly, the present invention relates to nucleic acid molecules, including fusion constructs, having catalytic activity and the use of same in glycosylation engineering of host cells to generate polypeptides with improved therapeutic properties, including antibodies with increased Fc receptor binding and increased effector function.
Abstract translation: 本发明涉及蛋白质的糖基化工程领域。 更具体地说，本发明涉及具有催化活性的核酸分子，包括具有催化活性的核糖分子，以及其在宿主细胞的糖基化工程中的用途，以产生具有改善的治疗性质的多肽，包括具有增加的Fc受体结合和增加的效应子功能的抗体。
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公开(公告)号：WO03091431A8
公开(公告)日：2005-02-17
申请号：PCT/JP0305464
申请日：2003-04-28
Applicant: KIRIN BREWERY , AIST , KOBAYASHI KAZUO , KITAGAWA YOSHINORI , KOMEDA TOSHIHIRO , KAWASHIMA NAGAKO , JIGAMI YOSHIFUMI , CHIBA YASUNORI
Inventor： KOBAYASHI KAZUO , KITAGAWA YOSHINORI , KOMEDA TOSHIHIRO , KAWASHIMA NAGAKO , JIGAMI YOSHIFUMI , CHIBA YASUNORI
IPC: C07K16/24 , C12N1/14 , C12N1/19 , C12N9/00 , C12N9/02 , C12N9/04 , C12N9/10 , C12N9/24 , C12N9/60 , C12N9/88 , C12P19/04 , C12P19/26 , C12P21/00 , C12P21/02 , C12N15/09 , C12N9/50
CPC classification number: C12P19/26 , C07K16/243 , C07K2317/21 , C12N1/14 , C12N9/0006 , C12N9/0008 , C12N9/1048 , C12N9/1051 , C12N9/2488 , C12N9/60 , C12N9/88 , C12N9/93 , C12P21/02 , C12Y101/01085 , C12Y101/03013 , C12Y102/01012 , C12Y302/01024 , C12Y402/01019 , C12Y603/02006
Abstract: It is intended to provide a process for producing a glycoprotein having a mammalian type sugar chain characterized by comprising transferring and expressing alpha-1,2-mannosidase in a sugar chain biosynthesis pathway gene mutant of a methylotroph yeast under the control by a potent promoter, culturing in a medium the methylotroph yeast cells having a foreign gene transferred thereinto, and then harvesting the glycoprotein having the mammalian type sugar chain from the culture medium. Using a newly bred methylotroph yeast carrying a sugar chain mutation, a neutral sugar chain identical with a high mannose type sugar chain produced by mammalian cells such as human cells or a glycoprotein having such a neutral sugar chain can be produced in a large amount at a high purity. By transferring a gene in a mammalian type sugar chain biosynthesis pathway into the above-described mutant, a mammalian type sugar chain such as a hybrid sugar chain or a complex sugar chain or a protein having a mammalian type sugar chain can be efficiently produced.
Abstract translation: 旨在提供一种具有哺乳动物型糖链的糖蛋白的制备方法，其特征在于包括通过有效启动子控制的甲基营养酵母的糖链生物合成途径基因突变体中转移和表达α-1,2-甘露糖苷酶， 在培养基中培养具有转移外源基因的甲基营养酵母细胞，然后从培养基中收获具有哺乳动物型糖链的糖蛋白。 使用携带糖链突变的新繁殖的甲基营养酵母，可以以大量的生成与哺乳动物细胞如人细胞或具有中性糖链的糖蛋白相同的高甘露糖型糖链的中性糖链 高纯度 通过将哺乳动物型糖链生物合成途径中的基因转移到上述突变体中，可以有效地制备哺乳动物型糖链如杂合糖链或复合糖链或具有哺乳动物型糖链的蛋白质。
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公开(公告)号：WO2003091431A1
公开(公告)日：2003-11-06
申请号：PCT/JP2003/005464
申请日：2003-04-28
IPC: C12N15/09
CPC classification number: C12P19/26 , C07K16/243 , C07K2317/21 , C12N1/14 , C12N9/0006 , C12N9/0008 , C12N9/1048 , C12N9/1051 , C12N9/2488 , C12N9/60 , C12N9/88 , C12N9/93 , C12P21/02 , C12Y101/01085 , C12Y101/03013 , C12Y102/01012 , C12Y302/01024 , C12Y402/01019 , C12Y603/02006
Abstract: 本発明は、メチロトロフ酵母の糖鎖生合成系酵素遺伝子変異株に強力なプロモーターの支配下にてα-1,2-マンノシダーゼを導入・発現させ、更に異種遺伝子を導入したメチロトロフ酵母細胞を培地に培養し、培養物より哺乳類型糖鎖を有する糖蛋白質を得ることを特徴とする、哺乳類型糖鎖を有する糖蛋白質の製造方法を提供する。新規に育種した糖鎖変異メチロトロフ酵母を用い、ヒトなど哺乳類細胞の生産するハイマンノース型と同一の中性糖鎖、あるいは同一の中性糖鎖を有する糖蛋白質を多量かつ純度よく生産することができる。また更に当該変異株に哺乳類型糖鎖の生合成系遺伝子を導入することにより、ハイブリッド型、複合型等の哺乳類型糖鎖、あるいは哺乳類型糖鎖を有する蛋白質を効率的に生産することができる。
Abstract translation: 旨在提供一种具有哺乳动物型糖链的糖蛋白的制备方法，其特征在于包括通过有效启动子控制的甲基营养酵母的糖链生物合成途径基因突变体中转移和表达α-1,2-甘露糖苷酶， 在培养基中培养具有转移外源基因的甲基营养酵母细胞，然后从培养基中收获具有哺乳动物型糖链的糖蛋白。 使用携带糖链突变的新繁殖的甲基营养酵母，可以以大量的生成与哺乳动物细胞如人细胞或具有中性糖链的糖蛋白相同的高甘露糖型糖链的中性糖链 高纯度 通过将哺乳动物型糖链生物合成途径中的基因转移到上述突变体中，可以有效地制备哺乳动物型糖链如杂合糖链或复合糖链或具有哺乳动物型糖链的蛋白质。
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