公开(公告)号：WO2009134418A3

公开(公告)日：2010-01-21

申请号：PCT/US2009002677

申请日：2009-04-30

Applicant: FOX CHASE CANCER CT ,  KATZ RICHARD A ,  SKALKA ANNA MARIE

Inventor： KATZ RICHARD A ,  SKALKA ANNA MARIE

IPC: C12Q1/68 ,  C12N5/00

CPC classification number: C12N15/111 ,  C12N2320/12

Abstract: A high throughput RNAi-based assay for identify factors involved in maintaining epigenetic silencing is disclosed. The assay measures reactivation of a silent reporter gene in cells, resulting from RNAi-based knockdown of target mRNA. RNAi-based screening of these silent reporter cells has identified known enzymes that place or remove epigenetic marks on histones, as well as non-enzymatic proteins that function in silencing or in transfer of marks during S-phase. In addition, the screen has been used to identify a number of novel gene products involved in epigenetic silencing, which are also disclosed.

Abstract translation: 公开了用于识别维持表观遗传学沉默的因素的高通量RNAi测定。 该测定法测定细胞中无声报道基因的再活化，由基于RNAi的靶mRNA敲低引起。 这些无声报道细胞的基于RNAi的筛选已经鉴定了在组蛋白中放置或除去表观遗传标记的已知酶，以及在S期期间沉默或转移标记的非酶蛋白质。 此外，该屏幕已经用于鉴定参与表观遗传学沉默的许多新的基因产物，这也被公开。