中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

157 records (took 0.027 seconds)

    DIGITAL DROPLET PCR SYSTEM
    1.
    发明申请
    DIGITAL DROPLET PCR SYSTEM 审中-公开

    公开(公告)号：WO2023069109A1

    公开(公告)日：2023-04-27

    申请号：PCT/US2021/056135

    申请日：2021-10-22

    Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

    Inventor： SHKOLNIKOV, Viktor ,  CUMBIE, Michael

    IPC: C12Q1/686 ,  B81B7/02 ,  C12M1/02

    Abstract: A digital droplet PCR system is described. The digital droplet PCR system comprises a microfluidic cartridge comprising: a plurality of sample droplet generators, wherein each sample droplet generator is operable to partition a PCR mixture into a plurality of aqueous droplets dispersed in a carrier liquid, and wherein at least one sample droplet generator is operable to partition a PCR mixture that is different to a PCR mixture that is partitioned by at least one other sample droplet generator; a thermocycling chamber comprising an embedded heater, an inlet configured to receive the plurality of aqueous droplets from the plurality of sample droplet generators, and an outlet; and an optical readout zone fluidly connected to the outlet of the thermocycling chamber; and a pressure-actuated pump configured to couple to and cause fluid flow through the microfluidic cartridge. A method of performing digital droplet PCR is also described.

    MECHANICAL CELL LYSIS IN DIGITAL MICROFLUIDIC DEVICES
    2.
    发明申请
    MECHANICAL CELL LYSIS IN DIGITAL MICROFLUIDIC DEVICES 审中-公开

    公开(公告)号：WO2023287404A1

    公开(公告)日：2023-01-19

    申请号：PCT/US2021/041511

    申请日：2021-07-14

    Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

    Inventor： GOVYADINOV, Alexander ,  SHKOLNIKOV, Viktor

    IPC: G01N27/26 ,  G01N33/487 ,  B81B1/00

    Abstract: This disclosure relates to mechanical cell lysis in a digital microfluidic (DMF) platform, which includes an embedded thin film resistor to generate vapor bubbles in a drop so as to perform the mechanical cell lysis. Cell lysis is a necessary step for single cell analysis (e.g., genomics, transcriptomics, proteomics, and metabolomics). Performing lysis at a single cell level in a DMF platform allows for a complex, automated, reprogrammable workflow to be performed simultaneously or sequentially to a large number of cells. Mechanical lysis is advantageous over chemical lysis because no lysing reagent is introduced into the lysis, which may degrade the analytes of interest and interfere with a downstream analysis.

    PARTICLE DETECTION VIA RESONANT FREQUENCIES
    6.
    发明申请
    PARTICLE DETECTION VIA RESONANT FREQUENCIES 审中-公开

    公开(公告)号：WO2022055480A1

    公开(公告)日：2022-03-17

    申请号：PCT/US2020/049858

    申请日：2020-09-09

    Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

    Inventor： SHKOLNIKOV, Viktor ,  YU, Joshua M. ,  SMITH, Matthew David ,  PUGLIESE, Roberto A.

    IPC: C12M3/00 ,  B81B7/02

    Abstract: In one example in accordance with the present disclosure, a particle detection system is described. The particle detection system includes a microfluidic channel through which fluid is to flow. The fluid includes particles. The particle detection system also includes a sensing circuit to output a resonant frequency. The sensing circuit includes a pair of electrodes disposed within the microfluidic channel. Contents of a volume between the pair of electrodes changes a capacitance between the pair of electrodes. A change in the capacitance changes the resonant frequency output by the sensing circuit. The particle detection system also includes a controller to determine the contents of the volume based on the resonant frequency.

    SORTING A DROPLET INCLUDING A BIOLOGIC SAMPLE
    10.
    发明申请
    SORTING A DROPLET INCLUDING A BIOLOGIC SAMPLE 审中-公开

    公开(公告)号：WO2021112841A1

    公开(公告)日：2021-06-10

    申请号：PCT/US2019/064505

    申请日：2019-12-04

    Applicant: HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P.

    Inventor： GOVYADINOV, Alexander ,  SHKOLNIKOV, Viktor

    IPC: C12Q1/6851 ,  C12Q1/6809 ,  B81B7/02

    Abstract: Examples herein involve sorting a droplet including a biologic sample. In a particular example, sorting a droplet including a biologic sample includes generating a droplet including a biologic sample and a pH sensitive surfactant, and heating a nucleic acid molecule in the biologic sample. The pH sensitive surfactant may change the surface tension of the droplet responsive to amplification of the nucleic acid molecule. The droplet may be sorted into one of a plurality of sorting lanes based on the surface tension of the droplet, where a sorting lane among the plurality of sorting lanes is associated with droplets including the amplified nucleic acid molecule. A determination of whether the droplet includes the amplified nucleic acid molecule may be performed by detecting passage of the droplet in one of the plurality of sorting lanes.

Patent Agency Ranking