公开(公告)号：WO2016007975A1

公开(公告)日：2016-01-14

申请号：PCT/VN2014/000004

申请日：2014-07-07

申请人： HOANG, Duong Thanh

发明人： HOANG, Duong Thanh ,  HOANG, Kim Phuc

IPC分类号： A01K67/033 ,  C12N15/113

CPC分类号： A01K67/0339 ,  A01K2207/05 ,  A01K2217/05 ,  A01K2217/20 ,  A01K2217/206 ,  A01K2227/706 ,  A01K2267/02 ,  C12N15/111 ,  C12N15/113 ,  C12N15/85 ,  C12N2310/14

摘要： An all male Culicide mosquito population is created by knocking down its Transformer-2 gene, causing the dysfunction of X chromosome-bearing sperm, hence producing severe biased male progenies. Unlike previous methods, we recently discovered that the Tra-2 knockdown also results in female- specific zygotes lethality (XX). This art is therefore also designed to kill early female zygotes (XX) that may have survived the previous knockdown, and the all male progenies are created only when an antibiotic substance has been added into food and drink to feed mosquitoes. The strict limit of the antibiotic exposure time allows mosquito-adapted Wolbachia bacteria to survive. Selected Wolbachia bacteria may induce cytoplasmic incompatibility (CI) of up to 100%. All the progenies are therefore genetically males, which cause sterility when outcrossing with females infected by another Wolbachia strain (bidirectional CI) or are uninfected (unidirectional CI) in natural environment.

摘要翻译： 通过击倒其Transformer-2基因，导致X染色体携带精子的功能障碍，从而产生严重偏倚的男性后代，所有男性Culicide蚊子群体都是创造的。 与以前的方法不同，我们最近发现Tra-2敲除也导致女性特异性合子致死率（XX）。 因此，这种艺术也被设计用于杀死可能在之前的击倒中幸存的早期雌性受精卵（XX），并且所有男性后代仅在将抗生素物质添加到食物和饮料中才能产生蚊子时产生。 抗生素暴露时间的严格限制允许蚊子适应的Wolbachia细菌存活。 所选Wolbachia细菌可诱导高达100％的细胞质不相容性（CI）。 所有后代因此是遗传性雄性，当与自然环境中的另一种Wolbachia菌株（双向CI）感染的雌性或未感染（单向CI）异交时，其导致不育。

公开(公告)号：WO2012129577A1

公开(公告)日：2012-09-27

申请号：PCT/VN2011/000011

申请日：2011-12-29

申请人： HOANG, Duong Thanh ,  HOANG, Kim Phuc

发明人： HOANG, Duong Thanh ,  HOANG, Kim Phuc

IPC分类号： A01K67/033 ,  C12N15/09 ,  C12N15/113 ,  C12N15/85

CPC分类号： C12N15/113 ,  A01K67/0333 ,  A01K67/0339 ,  A01K2217/058 ,  A01K2227/706 ,  A01K2267/02 ,  C12N2310/14 ,  C12N2330/51

摘要： The present invention develops a novel method for controlling mosquito populations. Culicinae mosquitoes carrying one or more loci of transformant Tra-2 RNAi constructs which target to mosquito Transformer-2 locus in respective or none respective Culicinae mosquitoes. Tra-2 sequences used to assemble Tra-2 RNAi recombinant constructs are Tra-2 gene sequences of Culicinae mosquitoes and can be derived from endogenous or exogenous sequences. The Tra-2 RNAi expression is conditional, wherein the expression causing a knockdown effect into the endogenous Tra-2 gene results in mortality of X (m) chromosome bearing sperms and produces maleness mosquito population in the nature environmental of the species.

摘要翻译： 本发明开发了一种控制蚊子种群的新方法。 携带一个或多个转基因Tra-2 RNAi构建体的Culicinae蚊子，其针对蚊子Transformer-2基因座在相应的或没有相应的Culicinae蚊子中。 用于组装Tra-2 RNAi重组构建物的Tra-2序列是Culicinae蚊子的Tra-2基因序列，并且可以衍生自内源或外源序列。 Tra-2 RNAi表达是有条件的，其中引起内源性Tra-2基因的敲低作用的表达导致带有X（m）染色体的精子的死亡率并且在该物种的自然环境中产生恶性的蚊子群体。