公开(公告)号：WO2021217104A1

公开(公告)日：2021-10-28

申请号：PCT/US2021/029008

申请日：2021-04-23

Applicant: INSCRIPTA, INC.

Inventor： BELGRADER, Phillip ,  SILTANEN, Christian ,  WATTERSON, William ,  DURA, Burak ,  CHABANSKY, Bruce ,  STUMBO, David ,  SMITH, Eric ,  BERNATE, Jorge

IPC: C12M1/00 ,  C12M3/00 ,  C12M1/26 ,  C12N15/87 ,  C12N15/90

Abstract: This invention relates to compositions of matter, methods, modules and instruments for automated mammalian cell growth and mammalian cell transduction followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：WO2019068062A1

公开(公告)日：2019-04-04

申请号：PCT/US2018/053671

申请日：2018-09-30

Applicant: INSCRIPTA, INC.

Inventor： BERNATE, Jorge ,  MASQUELIER, Don ,  BELGRADER, Phillip

IPC: A61K9/50 ,  A61K48/00 ,  A61N1/30 ,  A61N1/32 ,  C12M1/42 ,  C12N13/00 ,  C12N15/87 ,  C12N15/867

Abstract: In an illustrative embodiment, automated instruments comprising one or more flow- through electroporation devices or modules are provided to automate transformation of nucleic acids in live cells.

公开(公告)号：WO2021217106A1

公开(公告)日：2021-10-28

申请号：PCT/US2021/029011

申请日：2021-04-24

Applicant: INSCRIPTA, INC.

Inventor： BELGRADER, Phillip ,  BADE, Nathan ,  SILTANEN, Christian ,  MIR, Aamir ,  CHEN, Xi-Jun ,  MOK, Janine ,  DURA, Burak ,  CHABANSKY, Bruce ,  STUMBO, David ,  SMITH, Eric ,  BERNATE, Jorge

IPC: C12N15/11 ,  C12M1/00 ,  C12M1/36 ,  C12N9/22

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：WO2019006436A1

公开(公告)日：2019-01-03

申请号：PCT/US2018/040519

申请日：2018-06-30

Applicant: INSCRIPTA, INC.

Inventor： MASQUELIER, Don ,  BELGRADER, Phillip ,  BERNATE, Jorge ,  GILL, Ryan ,  NESS, Kevin

IPC: C12N15/10 ,  G01N35/04 ,  G01N35/00

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

公开(公告)号：WO2020037057A1

公开(公告)日：2020-02-20

申请号：PCT/US2019/046526

申请日：2019-08-14

Applicant: INSCRIPTA, INC.

Inventor： SPINDLER, Eileen ,  HIDDESSEN, Amy ,  BELGRADER, Phillip ,  JOHNSON, Charles ,  DAVIS, Clint

IPC: A61K35/12 ,  A61K35/17 ,  C12N15/90 ,  C12N5/0783

Abstract: The present disclosure provides methods, automated modules, and instruments for enrichment of live cells that have been edited by nucleic acid-guided nuclease genome editing. The disclosure provides improved methods and modules–including high throughput methods and modules–for enriching for cells that have been subjected to editing.

公开(公告)号：WO2020005383A1

公开(公告)日：2020-01-02

申请号：PCT/US2019/030085

申请日：2019-04-30

Applicant: INSCRIPTA, INC.

Inventor： BELGRADER, Phillip ,  MASQUELIER, Don ,  CHABANSKY, Bruce ,  BERNATE, Jorge ,  GARST, Andrew ,  FOX, Richard

IPC: B01D25/00 ,  B01D25/22 ,  B01D61/18

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods — including high throughput methods — for screening cells that have been subjected to editing and identifying cells that have been properly edited.

公开(公告)号：WO2019190874A1

公开(公告)日：2019-10-03

申请号：PCT/US2019/023342

申请日：2019-03-21

Applicant: INSCRIPTA, INC.

Inventor： MASQUELIER, Don ,  BELGRADER, Phillip

IPC: C12N1/36 ,  C12N5/07

Abstract: The present disclosure relates to methods and devices for automated control of cell growth rates where cell growth is measured in situ and the devices can be used as a stand-alone device or as a module in an automated environment, e.g., as one module in a multi-station or multi-module cell processing environment. The cell growth device comprises a temperature-controlled vial, a motor assembly to spin the vial, a spectrophotometer for measuring, e.g., OD of the cells in the vial, and a processor to accept input from a user and control the growth rate of the cells.

公开(公告)号：WO2020081149A3

公开(公告)日：2020-04-23

申请号：PCT/US2019/047135

申请日：2019-08-20

Applicant: INSCRIPTA, INC.

Inventor： SPINDLER, Eileen ,  WAGNER, Isaac ,  DAVIS, Clint ,  SWAVOLA, Julia ,  BELGRADER, Phillip

IPC: C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N9/22

Abstract: The present disclosure provides modules, instruments and methods to enrich for cells edited via nucleic acid-guided nuclease editing of live cells.

公开(公告)号：WO2020051323A1

公开(公告)日：2020-03-12

申请号：PCT/US2019/049735

申请日：2019-09-05

Applicant: INSCRIPTA, INC.

Inventor： BERNATE, Jorge ,  MASQUELIER, Don ,  BELGRADER, Phillip ,  CHABANSKY, Bruce

IPC: B01L3/00 ,  C12M1/12 ,  C12M1/32 ,  C12M1/34 ,  C12M1/36 ,  C12M3/06

Abstract: The present disclosure provides a cell growth, buffer exchange, and/or cell concentration/filtration device that may be used as a stand-alone device or as a module configured to be used in an automated multi-module cell processing environment.

公开(公告)号：WO2020037051A1

公开(公告)日：2020-02-20

申请号：PCT/US2019/046515

申请日：2019-08-14

Applicant: INSCRIPTA, INC.

Inventor： MASQUELIER, Don ,  BELGRADER, Phillip ,  GARST, Andrew ,  FOX, Richard ,  ESTES, Matthew ,  CHABANSKY, Bruce

IPC: B32B37/14 ,  C12M1/34 ,  C12M3/06

Abstract: The present disclosure provides instruments, modules and methods for improved detection of edited cells following nucleic acid-guided nuclease genome editing. The disclosure provides improved automated instruments that perform methods—including high throughput methods—for screening cells that have been subjected to editing and identifying cells that have been properly edited.