公开(公告)号：WO2023076134A1

公开(公告)日：2023-05-04

申请号：PCT/US2022/047519

申请日：2022-10-24

Applicant: INSCRIPTA, INC.

Inventor： HORWITZ, Andrew ,  APEL, Amanda Reider ,  TARASOW, Theodore M. ,  MIRSIAGHI, Mona ,  GRAVES, Christopher ,  BARBIERI, Charles

IPC: C12N15/10 ,  C12Q1/04 ,  C12N15/70 ,  C12N15/81

Abstract: The disclosure provides examples of multi-parallel processes that are used to measure strain fitness of genotypically different strains of a microorganism in one or more bioreactors and the selection of strains that have improved genotypes.

公开(公告)号：WO2022146498A1

公开(公告)日：2022-07-07

申请号：PCT/US2021/048578

申请日：2021-09-01

Applicant: INSCRIPTA, INC.

Inventor： KIM, Juhan ,  MIJTS, Benjamin

IPC: C12N9/22 ,  C12N9/78 ,  C12N15/11 ,  C12N15/85

Abstract: The present disclosure provides engineered nucleic acid-guided nickases and optimized scaffolds for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：WO2022146497A1

公开(公告)日：2022-07-07

申请号：PCT/US2021/048566

申请日：2021-08-31

Applicant: INSCRIPTA, INC.

Inventor： KIM, Juhan ,  MIJTS, Benjamin ,  MIR, Aamir

IPC: C12N9/22 ,  C12N15/09 ,  C12N15/10 ,  C12N15/113 ,  C12N15/52

Abstract: The present disclosure provides new RNA-guided nuclease systems and engineered nickases for making rational, direct edits to nucleic acids in live cells.

公开(公告)号：WO2022125329A1

公开(公告)日：2022-06-16

申请号：PCT/US2021/061156

申请日：2021-11-30

Applicant: INSCRIPTA, INC. [US]/[US]

Inventor： MIR, Aamir

IPC: C12N9/12 ,  C12N9/22 ,  C12N15/113

Abstract: The present disclosure provides compositions of matter, methods and instruments for nucleic acid-guided nickase/reverse transcriptase fusion editing in live cells. Editing efficiency is improved using fusion proteins (e.g., the nickase-RT fusion) that retain certain characteristics of nucleic acid-directed nucleases (e.g., the binding specificity and ability to cleave one or more DNA strands in a targeted manner) combined with reverse transcriptase activity. Editing cassettes are employed, comprising a gRNA and a repair template where the 3' end of the repair template is protected from degradation.

公开(公告)号：WO2022072878A1

公开(公告)日：2022-04-07

申请号：PCT/US2021/053235

申请日：2021-10-01

Applicant: INSCRIPTA, INC.

Inventor： HALWEG-EDWARDS, Andrea ,  HRAHA, Thomas ,  YERRAMSETTY, Krishna ,  LAMBERT, Shea ,  GANDER, Miles ,  ESTES, Matthew David ,  SANADA, Chad Douglas ,  WAGNER, Isaac David ,  HARDENBOL, Paul

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C40B30/00 ,  C40B40/06 ,  C40B40/08

Abstract: Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population.

公开(公告)号：WO2021217106A1

公开(公告)日：2021-10-28

申请号：PCT/US2021/029011

申请日：2021-04-24

Applicant: INSCRIPTA, INC.

Inventor： BELGRADER, Phillip ,  BADE, Nathan ,  SILTANEN, Christian ,  MIR, Aamir ,  CHEN, Xi-Jun ,  MOK, Janine ,  DURA, Burak ,  CHABANSKY, Bruce ,  STUMBO, David ,  SMITH, Eric ,  BERNATE, Jorge

IPC: C12N15/11 ,  C12M1/00 ,  C12M1/36 ,  C12N9/22

Abstract: This invention relates to compositions of matter, methods, modules and automated, end-to-end closed instruments for automated mammalian cell growth, reagent bundle creation and mammalian cell transfection followed by nucleic acid-guided nuclease editing in live mammalian cells.

公开(公告)号：WO2021154480A1

公开(公告)日：2021-08-05

申请号：PCT/US2021/012868

申请日：2021-01-10

Applicant: INSCRIPTA, INC.

Inventor： ABRAHAM, Christopher

IPC: C12M3/00 ,  C12N9/22 ,  C12N15/113

Abstract: In an illustrative embodiment, the present disclosure provides methods, systems and/or instruments for the automated editing of immune cells for chimeric antigen receptor therapies.

公开(公告)号：WO2021071746A1

公开(公告)日：2021-04-15

申请号：PCT/US2020/053873

申请日：2020-10-01

Applicant: INSCRIPTA, INC.

Inventor： GARST, Andrew

IPC: C12N9/22 ,  C12N15/10 ,  C12N15/11

Abstract: The present disclosure provides compositions and methods to increase the percentage of edited cells in a cell population when employing nucleic-acid guided editing, as well as automated multi-module instruments for performing these methods.

公开(公告)号：WO2019006436A1

公开(公告)日：2019-01-03

申请号：PCT/US2018/040519

申请日：2018-06-30

Applicant: INSCRIPTA, INC.

Inventor： MASQUELIER, Don ,  BELGRADER, Phillip ,  BERNATE, Jorge ,  GILL, Ryan ,  NESS, Kevin

IPC: C12N15/10 ,  G01N35/04 ,  G01N35/00

Abstract: In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells.

公开(公告)号：WO2022150269A1

公开(公告)日：2022-07-14

申请号：PCT/US2022/011052

申请日：2022-01-03

Applicant: INSCRIPTA, INC.

Inventor： KIM, Juhan ,  MIJTS, Benjamin ,  MIR, Aamir

IPC: C12N9/22 ,  C12P19/34 ,  C12N15/09 ,  C12N15/10 ,  C12N15/113 ,  C12N15/52

Abstract: The present disclosure provides new RNA-guided nucleases for making rational, direct edits to nucleic acids in live cells; specifically, the present disclosure provides Type V MAD nucleases (e.g., RNA-guided nucleases or RGNs) with altered PAM preferences and/or altered activity at different temperatures or fidelity, and/or varied nuclease activities; all changes that may increase the versatility of a nucleic acid-guided nuclease for certain editing tasks.