公开(公告)号：WO2016187466A1

公开(公告)日：2016-11-24

申请号：PCT/US2016/033348

申请日：2016-05-19

Applicant: INVENRA, INC.

Inventor： GLASER, Bryan ,  MEYVANTSSON, Ivar ,  GREEN, Roland ,  KAUFMAN, Kimberly ,  GREEN, Madison ,  GRIBB, Tye ,  PAINTER, John ,  HAMMER, Bonnie ,  SKOIEN, Allyson

IPC: C12N15/10 ,  C12N15/13 ,  C40B20/04 ,  C40B30/04 ,  C40B40/06

CPC classification number: C40B40/06 ,  C12N15/1075 ,  C12Q1/6806 ,  G01N33/6845 ,  G01N33/6854 ,  C12Q2537/149 ,  C12Q2537/159 ,  C12Q2563/149

Abstract: Methods and apparatus are provided for screening libraries of polypeptides linked to coding sequences for a biological activity. Libraries of nucleic acid molecules encoding candidate polypeptides, such as antibodies, can be amplified, expressed, and linked to beads. The library allows for efficient high-throughput screening to identify polypeptides having a desired biological activity. In some aspects, amplification, expression, and/or screening is performed in a high-density pico-, nano-, or micro-well plate format. Methods and compositions for efficient expression and screening of antibodies are likewise provided.

Abstract translation: 提供了用于筛选与用于生物活性的编码序列连接的多肽的文库的方法和装置。 编码候选多肽例如抗体的核酸分子的文库可以被扩增，表达并连接到珠子上。 该文库允许有效的高通量筛选来鉴定具有所需生物活性的多肽。 在一些方面，扩增，表达和/或筛选以高密度微晶，纳米或微孔板格式进行。 同样提供用于有效表达和筛选抗体的方法和组合物。