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    METHOD OF DETERMINING THE MOLECULAR BINDING BETWEEN LIBRARIES OF MOLECULES
    3.
    发明申请
    METHOD OF DETERMINING THE MOLECULAR BINDING BETWEEN LIBRARIES OF MOLECULES 审中-公开
    确定分子文库间分子结合的方法

    公开(公告)号：WO2018005720A1

    公开(公告)日：2018-01-04

    申请号：PCT/US2017/039862

    申请日：2017-06-29

    Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

    Inventor： GRISWOLD, Kettner John Frederick ,  FANG, Neng

    IPC: C12Q1/68 ,  C07K16/00 ,  C40B30/04 ,  C40B40/08 ,  C40B50/06

    CPC classification number: C12N15/1062 ,  C12Q1/68 ,  C12Q1/6869 ,  G01N33/6845 ,  G01N2458/10 ,  C12Q2565/514

    Abstract: A method for determining the molecular binding between a target molecule library and a binder molecule library is provided including contacting the target molecule library comprising a plurality of mRNA-protein conjugates with the binder molecule library comprising a plurality of mRNA-protein conjugates under conditions where one or more proteins in the target library bind to one or more proteins in the binder library, and determining the molecular binding of the target and binder protein pairs by determining the sequences of the mRNAs conjugated to the target and binder protein pairs.

    Abstract translation: 提供了确定靶分子文库和结合分子文库之间的分子结合的方法，包括将包含多种mRNA-蛋白缀合物的靶分子文库与包含多个 在靶标文库中的一种或多种蛋白质与结合文库中的一种或多种蛋白质结合的条件下，通过确定与靶标结合的mRNA的序列以及确定靶标和结合蛋白对的分子结合以及 结合蛋白对。

    RECEPTOR LINKED REGENERATED CELLULOSE MEMBRANE AND METHODS FOR PRODUCING AND USING THE SAME
    4.
    发明申请
    RECEPTOR LINKED REGENERATED CELLULOSE MEMBRANE AND METHODS FOR PRODUCING AND USING THE SAME 审中-公开
    受体连接的再生纤维素膜及其制备和使用方法

    公开(公告)号：WO2017086668A1

    公开(公告)日：2017-05-26

    申请号：PCT/KR2016/013105

    申请日：2016-11-14

    Applicant: NB POSTECH, INC.

    Inventor： PARK, Joon Won ,  SEO, Sung Min ,  KO, Jong Myung

    IPC: G01N33/68 ,  G01N33/531 ,  G01N33/543

    CPC classification number: G01N33/531 ,  B01J20/28033 ,  B01J20/3274 ,  G01N33/00 ,  G01N33/543 ,  G01N33/54353 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6845

    Abstract: The present invention provides a regenerated cellulose membrane (RCM) that is useful in analysis of a sample for the presence of or the amount of a target molecule present in the sample. The invention also provides a method for producing and using the same. The RCM of the invention has a plurality of RCM functional groups on its surface in which a linker is covalently attached. The linker has a distal end and a proximal end, where the proximal end comprises at least one RCM linking functional group such that the RCM linking functional group is attached to the RCM functional group. The distal end of the linker comprises a receptor linking functional group that is used to covalently attach a receptor molecule. The receptor molecule is adapted for binding to a target molecule of interest when the target molecule is present in the sample. The RCM of the invention can be used for quantitative and/or qualitative analysis of a target molecule within the sample. The present invention also provides a method for producing and using the linker.

    Abstract translation: 本发明提供再生纤维素膜（RCM），其可用于分析样品中样品中存在的靶分子的存在或量。 本发明还提供了一种生产和使用它的方法。 本发明的RCM在其表面上具有多个RCM官能团，其中连接基共价连接。 接头具有远端和近端，其中近端包含至少一个RCM连接官能团，使得RCM连接官能团连接到RCM官能团。 接头的远端包含用于共价连接受体分子的受体连接官能团。 当目标分子存在于样品中时，受体分子适于结合目的靶分子。 本发明的RCM可以用于样品内靶分子的定量和/或定性分析。 本发明还提供了一种生产和使用接头的方法。

    MULTIPLEX ANALYSIS OF SINGLE CELL CONSTITUENTS
    5.
    发明申请
    MULTIPLEX ANALYSIS OF SINGLE CELL CONSTITUENTS 审中-公开
    单节电池组件的多重分析

    公开(公告)号：WO2017075265A1

    公开(公告)日：2017-05-04

    申请号：PCT/US2016/059195

    申请日：2016-10-27

    Applicant: THE BROAD INSTITUTE, INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY

    Inventor： GAUBLOMME, Jellert ,  REGEV, Aviv

    IPC: G01N1/30 ,  G01N33/58 ,  C12Q1/68 ,  C12N15/10

    CPC classification number: G01N33/58 ,  C12Q1/6869 ,  G01N33/6845 ,  G01N2458/10 ,  C12Q2535/122 ,  C12Q2563/179

    Abstract: The present invention relates to methods for high multiplex protein or cellular constituent analysis in single cells or single isolated aggregations of cellular constituents. The methods provide for embedding cells or isolated aggregations of cellular constituents in a hydrogel mesh and labeling of cellular constituents with labeling ligands linked to a nucleic acid tag. Cellular constituents can be determined using sequencing methods.

    Abstract translation: 本发明涉及用于在细胞成分的单细胞或单分离聚集体中进行高度多重蛋白质或细胞组分分析的方法。 该方法提供将细胞或分离的细胞成分聚集体包埋在水凝胶网中，并用与核酸标签连接的标记配体标记细胞成分。 细胞成分可以使用测序方法来确定。

    A METHOD OF DETECTING MOLECULES IN PROXIMITY TO A TARGET MOLECULE IN A SAMPLE
    6.
    发明申请
    A METHOD OF DETECTING MOLECULES IN PROXIMITY TO A TARGET MOLECULE IN A SAMPLE 审中-公开
    检测样品中目标分子的分子的方法

    公开(公告)号：WO2017040404A1

    公开(公告)日：2017-03-09

    申请号：PCT/US2016/049263

    申请日：2016-08-29

    Applicant: THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES

    Inventor： BAR, Daniel Z. ,  COLLINS, Francis

    IPC: G01N33/68

    CPC classification number: G01N33/6845

    Abstract: Antibody-based methods of detecting of molecules interacting with a target molecule, such as a nuclear or cytoplasmic protein, in a fixed and, optionally, permeabilized cell or tissue sample. In one example, the target molecule is bound by a primary antibody, and a secondary, peroxidase-conjugated antibody, then binds to the primary antibody. The peroxidase may also be conjugated the primary antibody. Biotin tyramide is added to the sample, and the peroxidase generates short-lived intermediates resulting in biotinylation of molecules adjacent to the peroxidase. Biotinylated molecules can be isolated from the sample by affinity interaction with avidin- or streptavidin, resulting in a fraction containing biotinylated molecules that are located in the sample in proximity to the target molecule. The fraction can be analyzed by mass-spectroscopy, Western blotting, sequencing and other techniques.

    Abstract translation: 在固定和任选的透化细胞或组织样品中检测与靶分子例如核或细胞质蛋白相互作用的分子的基于抗体的方法。 在一个实例中，靶分子被一级抗体结合，并且次级的，过氧化物酶缀合的抗体然后与一级抗体结合。 过氧化物酶也可以与一级抗体结合。 将生物素酪胺加入到样品中，过氧化物酶产生短暂的中间体，导致与过氧化物酶相邻的分子的生物素化。 生物素化的分子可以通过与抗生物素蛋白或链霉抗生物素蛋白亲和力相互作用从样品中分离出来，导致含有生物素化分子的部分位于样品中靠近靶分子。 该分数可以通过质谱，蛋白质印迹，测序和其他技术进行分析。

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