公开(公告)号：WO1992007934A1

公开(公告)日：1992-05-14

申请号：PCT/US1991007887

申请日：1991-10-31

Applicant: IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC.

Inventor： IOWA STATE UNIVERSITY RESEARCH FOUNDATION, INC. ,  KRAMER, Theodore, T.

IPC: C12N01/00

CPC classification number: C12R1/42 ,  A61K39/0275 ,  A61K2039/522 ,  A61K2039/552 ,  C12N1/36 ,  Y02A50/482 ,  Y10S435/879

Abstract: Disclosed herein are methods for attenuating virulent Gram negative bacteria to produce avirulent bacteria. The methods comprise passaging the wild-type bacteria through phagocytic cells, such as macrophages or polymorphonuclear leukocytes, or through lysosomes derived from such cells, a sufficient number of times until the bacteria become avirulent to the animal host. The bacteria are preferably from the family Enterobacteracea and most preferably from the genus Salmonellae. The invention further comprises the avirulent bacteria produced by the methods, pure cultures of such bacteria, and methods of using the bacteria, preferably in a vaccine for administration to an animal host to induce an immune response to the wild-type Gram negative bacteria in the host.

Abstract translation: 本文公开了用于减毒毒性革兰氏阴性细菌以产生无毒力细菌的方法。 所述方法包括通过吞噬细胞（例如巨噬细胞或多形核白细胞）或通过来源于这些细胞的溶酶体传代野生型细菌，直到细菌无毒给动物宿主为止。 细菌优选来自肠杆菌属，最优选来自沙门氏菌属。 本发明还包括通过这些方法产生的无毒力细菌，这种细菌的纯培养物，以及使用细菌的方法，优选在疫苗中给动物宿主诱导对野生型革兰氏阴性菌的免疫应答 主办。