公开(公告)号：WO2007038145A3

公开(公告)日：2007-10-11

申请号：PCT/US2006036668

申请日：2006-09-08

Applicant: LARGE SCALE BIOLOGY CORP

Inventor： LINDBO JOHN A ,  SMITH MARK L ,  PALMER KENNETH E

IPC: C12N7/01

CPC classification number: C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K39/385 ,  A61K2039/5258 ,  A61K2039/55566 ,  A61K2039/6075 ,  A61K2039/625 ,  C07K2319/00 ,  C07K2319/22 ,  C07K2319/60 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2770/00022

Abstract: Compositions and methods to utilize coat proteins of tobacco mosaic virus (TMV) as a scaffold with commonly available linker chemistries and surface antigens to enhance immune responses when used as a vaccination; randomized library to introduce a reactive lysine or cysteine residue externally located at the amino-terminus of the coat protein. TMV coat protein modification allows a controlled stoichiometric biotinylation of the Introduced lysine or cysteine residues further allowing the attachment of antigens using streptavidin as a linker to the biotinylated coat protein. A TMV-protein complex vaccine prepared utilizing this invention results in enhanced immunogenicity and a detectable humoral immune response after only a single immunization in mice.

Abstract translation: 使用烟草花叶病毒（TMV）的外壳蛋白作为具有通常可用的接头化学物质和表面抗原的支架的组合物和方法，以在用作疫苗接种时增强免疫应答; 随机化文库引入外部位于外壳蛋白的氨基末端的反应性赖氨酸或半胱氨酸残基。 TMV外壳蛋白修饰允许引入的赖氨酸或半胱氨酸残基的受控化学计量生物素化进一步允许使用链霉抗生物素蛋白作为接头与生物素化的外壳蛋白连接抗原。 使用本发明制备的TMV-蛋白复合疫苗在小鼠中仅进行单次免疫后导致增强的免疫原性和可检测的体液免疫应答。

公开(公告)号：WO0107641A3

公开(公告)日：2001-08-09

申请号：PCT/US0020143

申请日：2000-07-20

Applicant: LARGE SCALE BIOLOGY CORP ,  FITZMAURICE WAYNE P ,  LINDBO JOHN A ,  PADGETT HAL S ,  POGUE GREGORY P

Inventor： FITZMAURICE WAYNE P ,  LINDBO JOHN A ,  PADGETT HAL S ,  POGUE GREGORY P

IPC: A01H1/04 ,  A01H5/12 ,  C12N15/10 ,  C12N15/82 ,  C12N15/83 ,  C12Q1/68

CPC classification number: C12N15/1079 ,  A01H1/04 ,  A01H5/12 ,  C12N15/1034 ,  C12N15/8203 ,  C12N15/8242 ,  C12N15/8257 ,  C12Q1/68

Abstract: The present invention relates to a method for using viral vectors to bear populations of sequence variants and using plant hosts to select to sequences that exhibit the desired traits.

Abstract translation: 本发明涉及使用病毒载体来承载序列变体种群并使用植物宿主选择显示所需性状的序列的方法。

公开(公告)号：WO0066743A9

公开(公告)日：2002-03-14

申请号：PCT/US0012380

申请日：2000-05-04

Applicant: LARGE SCALE BIOLOGY CORP ,  FITZMAURICE WAYNE P ,  POGUE GREGORY P ,  LINDBO JOHN A

Inventor： FITZMAURICE WAYNE P ,  POGUE GREGORY P ,  LINDBO JOHN A

IPC: A01H5/00 ,  C07K14/08 ,  C12N5/10 ,  C12N15/09 ,  C12N15/40 ,  C12N15/82 ,  C12N15/86 ,  C12N5/14

CPC classification number: C07K14/005 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/86 ,  C12N2770/00022 ,  C12N2770/00043

Abstract: The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183kDa replicase proteins further characterized in its ability to stabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.

Abstract translation: 本发明提供具有改变的病毒运动蛋白质和126 / 183kDa复制酶蛋白质的核酸序列，其进一步特征在于其稳定包含在表达改变的运动蛋白质的病毒中的转基因的能力。 本发明还提供表达改变的运动蛋白的病毒载体，用载体转化的细胞，以及被病毒载体感染的宿主植物。

公开(公告)号：WO03103605A2

公开(公告)日：2003-12-18

申请号：PCT/US0318247

申请日：2003-06-06

Applicant: LARGE SCALE BIOLOGY CORP

Inventor： MCCORMICK ALISON A ,  SMITH MARK L ,  PALMER KENNETH E ,  LINDBO JOHN A ,  NGUYEN LONG V ,  POGUE GREGORY P

IPC: A61K20060101 ,  A61K39/12 ,  A61K39/385 ,  A61K48/00 ,  C07K14/025 ,  C07K14/08 ,  C07K14/11 ,  C07K14/115 ,  C07K14/12 ,  C07K14/47 ,  C07K19/00 ,  C12N7/00 ,  C12N7/01 ,  C12N7/04 ,  C12N15/86 ,  A61K

CPC classification number: C07K14/005 ,  A61K39/0011 ,  A61K39/12 ,  A61K39/385 ,  A61K2039/5258 ,  A61K2039/55516 ,  A61K2039/6075 ,  A61K2039/627 ,  A61K2039/64 ,  C07K14/4748 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20034 ,  C12N2760/16122 ,  C12N2760/18022 ,  C12N2770/00022 ,  C12N2770/00023

Abstract: Herein described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

Abstract translation: 本文描述了制备由重新组装的病毒样颗粒（VLP）制成的疫苗的各种方法。 首先，将VLP分解成壳化中间种群。 每个壳化中间体经历例如独特的肽或核酸部分的化学共轭以形成分开的群体。 此后，将来自不同群体的几种（一种或多种）不同的壳化中间体中的每一种的预定量混合并连接，形成围绕核酸核心的完整的VLP，其由不同的包封中间体组成，使得重新组装的VLP显示 多于一种肽或核酸。 核酸可以作为单独的支架起作用，或者可以被工程化以在真核细胞中表达免疫调节蛋白。

公开(公告)号：WO0166778A2

公开(公告)日：2001-09-13

申请号：PCT/US0107355

申请日：2001-03-08

Applicant: LARGE SCALE BIOLOGY CORP ,  POGUE GREGORY P ,  LINDBO JOHN A ,  MCCULLOCH MICHAEL J ,  LAWRENCE JONATHAN E ,  GROSS CYNTHIA S ,  GARGER STEPHEN J

Inventor： POGUE GREGORY P ,  LINDBO JOHN A ,  MCCULLOCH MICHAEL J ,  LAWRENCE JONATHAN E ,  GROSS CYNTHIA S ,  GARGER STEPHEN J

IPC: A01H5/00 ,  A61K39/00 ,  C07K14/08 ,  C07K14/445 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/02 ,  C12N15/09 ,  C12N15/40 ,  C12N15/82 ,  C12P21/02 ,  C12P21/04 ,  C12N5/14 ,  C12N15/62

CPC classification number: C07K14/005 ,  A61K39/00 ,  C07K14/445 ,  C07K2319/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2770/00022

Abstract: The present invention relates to foreign peptide sequences fused to the amino-terminal of plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The foreign peptide sequences can be cleaved from the fusion proteins by proteolytic enzymes or chemical reagents. The foreign peptide sequences of the invention have many uses. Such uses include use as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, for use as vaccine antigens for the induction of protective immunity, including immunity against parasitic infections, for use as a protein involved in hormonal activity, or for use as a protein involved in immunoregulatory activity.

Abstract translation: 本发明涉及与植物病毒结构蛋白的氨基末端融合的外来肽序列及其制备方法。 融合蛋白在生物学含有的烟草花叶病毒的高水平植物中经济合成。 外源肽序列可以通过蛋白水解酶或化学试剂从融合蛋白中切割出来。 本发明的外源肽序列具有许多用途。 这样的用途包括用作诱导产生具有所需结合特性的抗体的抗原，例如保护性抗体，用作诱导保护性免疫的疫苗抗原，包括针对寄生虫感染的免疫，用作参与激素活性的蛋白质， 或用作参与免疫调节活性的蛋白质。

公开(公告)号：WO2005091753A3

公开(公告)日：2006-08-03

申请号：PCT/US2005010191

申请日：2005-03-25

Applicant: LARGE SCALE BIOLOGY CORP ,  MCCORMICK ALISON A ,  SMITH MARK L ,  PALMER KENNETH E ,  LINDBO JOHN A ,  NGUYEN LONG V ,  POGUE GREGORY P

Inventor： MCCORMICK ALISON A ,  SMITH MARK L ,  PALMER KENNETH E ,  LINDBO JOHN A ,  NGUYEN LONG V ,  POGUE GREGORY P

IPC: A61K48/00 ,  A61K39/385 ,  A61K47/48 ,  C07K14/025 ,  C07K14/08 ,  C07K14/11 ,  C07K14/12 ,  C07K14/16 ,  C07K14/47 ,  C12N7/04

CPC classification number: C07K14/005 ,  A61K39/385 ,  A61K47/646 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/53 ,  A61K2039/545 ,  A61K2039/6075 ,  A61K2039/62 ,  A61K2039/627 ,  A61K2039/64 ,  C07K14/4748 ,  C07K2319/00 ,  C12N7/00 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2740/16122 ,  C12N2760/16122 ,  C12N2760/18422 ,  C12N2770/00022 ,  C12N2770/36143

Abstract: Herein described are various methods for making a vaccine that are made of re­assembled virus like particles (VLP). First, the VLPs are disassembled into coat proteins or encapsidation intermediate populations. Each population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different coat proteins or encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different coat proteins such that the reassembled VLP displays more than one peptide or other molecule. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.

Abstract translation: 本文描述了制备由重组病毒样颗粒（VLP）制成的疫苗的各种方法。 首先，将VLP分解成外壳蛋白或衣壳化中间种群。 每个群体经历例如独特的肽或核酸部分的化学共轭以形成分开的群体。 此后，将来自不同群体的几种（一种或多种）不同外壳蛋白或壳化中间体中的每一种的预定量混合并结合，形成由不同外壳蛋白构成的核酸核心周围的完整VLP， 重组VLP显示多于一个肽或其他分子。 核酸可以作为单独的支架起作用，或者可以被工程化以在真核细胞中表达免疫调节蛋白。

公开(公告)号：WO03066809A2

公开(公告)日：2003-08-14

申请号：PCT/US0302958

申请日：2003-01-31

Applicant: LARGE SCALE BIOLOGY CORP ,  PADGETT HAL S ,  VAEWHONGS ANDREW A ,  VOJDANI FAKHRIEH S ,  SMITH MARK L ,  LINDBO JOHN A ,  FITZMAURICE WAYNE P

Inventor： PADGETT HAL S ,  VAEWHONGS ANDREW A ,  VOJDANI FAKHRIEH S ,  SMITH MARK L ,  LINDBO JOHN A ,  FITZMAURICE WAYNE P

IPC: C12N15/09 ,  C07K14/08 ,  C12N9/16 ,  C12N9/22 ,  C12N15/10 ,  C12N15/82 ,  C12P21/00 ,  C12Q1/34 ,  C12Q1/68 ,  C12N

CPC classification number: C12N15/102 ,  C07K14/005 ,  C12N9/22 ,  C12N15/1027 ,  C12N15/8203 ,  C12N15/8257 ,  C12N2770/00022

Abstract: We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.

Abstract translation: 我们在这里描述了限制性内切核酸酶及其用途。 限制性内切核酸酶可用于寻找单核苷酸多态性。 它们也可用于在不相同的多核苷酸序列之间重新分配序列变异的体外方法。

公开(公告)号：WO0107613A2

公开(公告)日：2001-02-01

申请号：PCT/US0020142

申请日：2000-07-20

Applicant: LARGE SCALE BIOLOGY CORP ,  CHAPMAN SEAN ,  DAWSON WILLIAM O ,  DONSON JONOTHAN ,  KUMAGAI MONTO H ,  LEWANDOWSKI DENNIS J ,  LINDBO JOHN A ,  POGUE GREGORY P ,  SHIVPRASAD SHAILAJA

Inventor： CHAPMAN SEAN ,  DAWSON WILLIAM O ,  DONSON JONOTHAN ,  KUMAGAI MONTO H ,  LEWANDOWSKI DENNIS J ,  LINDBO JOHN A ,  POGUE GREGORY P ,  SHIVPRASAD SHAILAJA

IPC: C07K14/61 ,  C07K14/805 ,  C07K14/82 ,  C07K16/30 ,  C12N9/32 ,  C12N15/12 ,  C12N15/13 ,  C12N15/18 ,  C12N15/55 ,  C12N15/82 ,  C12N5/10

CPC classification number: C12N9/2422 ,  C07K14/61 ,  C07K14/805 ,  C07K14/82 ,  C07K16/3061 ,  C07K2317/13 ,  C07K2317/622 ,  C12N15/8203 ,  C12N15/8216 ,  C12N15/8245 ,  C12N15/8257 ,  C12N15/8258

Abstract: The present invention provides a method for enhancing the production of RNAs or proteins in a plant host using either non-native 5' untranslated sequences or artificial leader sequences. Preferably, commercially useful proteins, polypeptides, or fusion products thereof are produced, such as, enzymes, antibodies, hormones, pharmaceuticals, vaccines, pigments, anti-microbial polypeptides, and the like. The non-native 5' untranslated enhancers may also be effective in many different types of transcription or translation systems, such as bacterial and animal systems.

Abstract translation: 本发明提供了使用非天然5'非翻译序列或人造前导序列来增强植物宿主中RNA或蛋白质产生的方法。 优选地，产生商业上有用的蛋白质，多肽或其融合产物，例如酶，抗体，激素，药物，疫苗，颜料，抗微生物多肽等。 非天然的5'非翻译增强子也可以在许多不同类型的转录或翻译系统中有效，例如细菌和动物系统。