公开(公告)号：WO1998011255A1

公开(公告)日：1998-03-19

申请号：PCT/US1997016246

申请日：1997-09-16

Applicant: LI-COR, INC.

Inventor： LI-COR, INC. ,  OOMMEN, Abraham ,  ROEMER, Steve

IPC: C12Q01/68

CPC classification number: C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/113 ,  C12Q2535/101

Abstract: The present invention provides a novel method for labeling and sequencing nucleic acid molecules, particularly DNA molecules in which an internally labeled, partially extended primer is elongated in a cycled primer extension reaction. An unlabeled DNA primer is contacted with a DNA template in the presence of suboptimal amounts of four dNTPs, one of which is labeled with a detectable marker which may be a fluorescent or visible fluorophor, and infrared fluorophor or a radioactive label. This small, labeled primer extension product is then transferred to a new reaction where chain terminated primer extension products for DNA analysis are prepared.

Abstract translation: 本发明提供了一种用于标记和测序核酸分子的新方法，特别是在循环引物延伸反应中内部标记的部分延伸的引物伸长的DNA分子。 未标记的DNA引物与DNA模板在次最佳量的四种dNTP存在下接触，其中一种用可检测的标记物标记，其可以是荧光或可见的荧光体，红外荧光或放射性标记。 然后将这种小的标记的引物延伸产物转移到新的反应中，其中制备用于DNA分析的链终止引物延伸产物。