公开(公告)号：WO2018231818A1

公开(公告)日：2018-12-20

申请号：PCT/US2018/037090

申请日：2018-06-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： DHINGRA, Dalia ,  CHIEN, Richard

IPC: C12Q1/6806

Abstract: Provided are methods, as well as compositions, kits, and systems for preparing optimized control nucleic acids (polynucleotides) having reduce nucleic acid damage. Provided nucleic acid compositions provide reduced artifacts as compared to nucleic acid compositions prepared by conventional methods. Provided compositions are useful control in a variety of applications, including, but not limited to sequencing workflows to effectively monitor sensitivity, accuracy and/or precision of data.

公开(公告)号：WO2016201142A1

公开(公告)日：2016-12-15

申请号：PCT/US2016/036763

申请日：2016-06-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： MONGAN, Ann ,  CHIEN, Richard ,  BRINZA, Dumitru ,  BRAMLETT, Kelli

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2600/156 ,  C12Q2525/191 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2565/514

Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

Abstract translation: 在一些实施方案中，本公开一般涉及方法以及相关系统，组合物，试剂盒，装置和计算机可读介质，其包含使用附加到多个多核苷酸的多个标签的多重分子标记过程。 标签具有特征，包括序列，长度和/或可检测部分，或唯一地识别其附着的多核苷酸分子的任何其它特征，并允许跟踪标记分子混合物中的单个标记分子。 例如，具有唯一标签序列的标签可以唯一地识别其所附着的单个多核苷酸，并将单个多核苷酸与混合物中的其他标记多核苷酸区分开。 在一些实施方案中，多重分子标记方法可用于产生错误校正的测序数据和用于检测以核酸样品中低丰度存在的靶多核苷酸。