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    METHODS FOR THE ISOLATION OF BIOMOLECULES AND USES THEREOF
    2.
    发明申请
    METHODS FOR THE ISOLATION OF BIOMOLECULES AND USES THEREOF 审中-公开
    用于分离生物分子的方法及其用途

    公开(公告)号：WO2017180897A1

    公开(公告)日：2017-10-19

    申请号：PCT/US2017/027459

    申请日：2017-04-13

    Applicant: NEW YORK GENOME CENTER

    Inventor： HOUCK-LOOMIS, Brian

    IPC: C12N15/00 ,  C12N15/10 ,  G01N1/34 ,  G01N1/40

    CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/101 ,  C12Q1/6874 ,  C12Q2527/119

    Abstract: Methods for preparing and utilizing tunable electrostatic capture ("TEC") ligands that have an ionizable function are provided. The electrostatic nature of the ionizable functionality of the TEC ligands can be "tuned" or adjusted to either reversibly bind or release a desired target anion, such as a biomolecule, by varying the pH and/or the ionic strength of the binding conditions and release conditions. The TEC ligands can be bound to a solid support to form TEC binding surfaces. TEC surfaces, ligands, solid supports and the accompanying methods and buffer systems can be used to isolate polyanions, such as nucleic acids, from materials, for example in a size-selective manner.

    Abstract translation: 提供了用于制备和利用具有可离子化功能的可调谐静电俘获（“TEC”）配体的方法。 TEC配体的可电离官能团的静电性质可以被“调整” 或调整为通过改变结合条件和释放条件的pH和/或离子强度来可逆地结合或释放期望的靶标阴离子，例如生物分子。 TEC配体可以结合到固体载体上以形成TEC结合表面。 TEC表面，配体，固体载体以及伴随的方法和缓冲体系可以用于从材料中例如以尺寸选择性的方式分离聚阴离子如核酸。

    NEXT-GENERATION SEQUENCING TO IDENTIFY ABO BLOOD GROUP
    3.
    发明申请
    NEXT-GENERATION SEQUENCING TO IDENTIFY ABO BLOOD GROUP 审中-公开
    下一代序列鉴定ABO血型

    公开(公告)号：WO2017160686A1

    公开(公告)日：2017-09-21

    申请号：PCT/US2017/022033

    申请日：2017-03-13

    Applicant: GEORGETOWN UNIVERSITY

    Inventor： HURLEY, Carolyn, K. ,  HOU, Lihua ,  NG, Jennifer

    IPC: G01N33/50 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/11 ,  C12N15/00

    CPC classification number: C12Q1/6881 ,  C12N9/1048 ,  C12Q1/6874 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/172

    Abstract: Provided are methods of phase-defined genotyping of both alleles of the glycosyltransferase (ABO) locus of a human subject. In certain embodiments the methods include a sequencing step using next-generation sequencing. In certain embodiments the methods include a sequencing step using sequencing-by-synthesis. In certain embodiments the methods further include the steps of comparing contiguous composite nucleotide sequences to a library of reference genomic sequences encoding a region comprising exon (6) and exon (7) of the ABO locus, and identifying individual contiguous composite nucleotide sequences as either (i) a sequence encoding a region comprising a known exon (6) and exon (7) of the ABO locus, or (ii) a sequence encoding a region comprising a novel exon (6) and/or exon (7) of the ABO locus. Also provided are kits for phase-defined genotyping of both alleles of the ABO locus of a human subject.

    Abstract translation: 提供了人受试者的糖基转移酶（ABO）基因座的两个等位基因的阶段定义基因分型的方法。 在某些实施方案中，所述方法包括使用下一代测序的测序步骤。 在某些实施方案中，所述方法包括使用合成测序的测序步骤。 在某些实施方案中，所述方法还包括以下步骤：将连续复合核苷酸序列与编码包含ABO基因座的外显子（6）和外显子（7）的区域的参照基因组序列文库进行比较，并且将单个连续复合核苷酸序列鉴定为 i）编码包含ABO基因座的已知外显子（6）和外显子（7）的区域的序列，或（ii）编码包含ABO的新外显子（6）和/或外显子（7）的区域的序列 轨迹。 还提供了用于对人类ABO基因座的两个等位基因进行阶段定义基因分型的试剂盒。

    COMPOSITE LIQUID CELL (CLC) SUPPORTS, AND METHODS OF MAKING AND USING THE SAME
    6.
    发明申请
    COMPOSITE LIQUID CELL (CLC) SUPPORTS, AND METHODS OF MAKING AND USING THE SAME 审中-公开
    复合液体细胞（CLC）支持物及其制备和使用方法

    公开(公告)号：WO2017033070A1

    公开(公告)日：2017-03-02

    申请号：PCT/IB2016/054248

    申请日：2016-07-15

    Applicant: GENCELL BIOSYSTEMS LTD.

    Inventor： DALTON, Mark

    IPC: B01L3/00 ,  G01N35/00

    CPC classification number: C12N15/1075 ,  B01L3/5085 ,  B01L3/50851 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/161 ,  C12Q1/6874 ,  G01N35/0099 ,  G01N35/1002

    Abstract: Composite liquid cell supports are provided. Aspects of the supports include: a plurality of CLC containers, wherein each CLC container is configured to hold a CLC and comprises a fluorophilic inner surface having a water contact angle of 80 degrees or greater. The fluorophilic inner surface may have a first contact angle with a fluorous carrier liquid which is less than a second contact angle with an encapsulating liquid that is immiscible with the carrier liquid. The supports find use in, among other applications, CLC systems and devices. Also provided are methods of preparing and using CLC arrays that include the CLC supports of the invention.

    Abstract translation: 提供复合液晶盒支架。 支撑件的方面包括：多个CLC容器，其中每个CLC容器被配置为保持CLC并且包括具有80度或更大的水接触角的富含荧光的内表面。 荧光内表面可以与氟载体液体具有与与载体液体不混溶的封装液体小于第二接触角的第一接触角。 支持在其他应用中可用于CLC系统和设备。 还提供了制备和使用包括本发明的CLC载体的CLC阵列的方法。

    DEVICES AND METHODS FOR DIAGNOSTICS BASED ON ANALYSIS OF NUCLEIC ACIDS
    8.
    发明申请
    DEVICES AND METHODS FOR DIAGNOSTICS BASED ON ANALYSIS OF NUCLEIC ACIDS 审中-公开
    基于核酸分析的诊断设备和方法

    公开(公告)号：WO2016123481A3

    公开(公告)日：2017-01-05

    申请号：PCT/US2016015645

    申请日：2016-01-29

    Applicant: RGA INT CORP ,  YU BENJAMIN ,  GHOURI AHMED ,  RAYNER GARY ,  SUGAVANAM RAGHU ,  PAPP ZOLTAN

    Inventor： YU BENJAMIN ,  GHOURI AHMED ,  RAYNER GARY ,  SUGAVANAM RAGHU ,  PAPP ZOLTAN

    IPC: C12Q1/68 ,  G06F19/22 ,  G06F19/28

    CPC classification number: G06F19/22 ,  C12Q1/68 ,  C12Q1/6874 ,  G06F19/18 ,  G06F19/28 ,  G16H50/20 ,  Y02A90/26 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2545/114

    Abstract: A condition can be diagnosed based on a symptom experienced by a subject and based on a biological sample including nucleic acids. Based on the symptom, a first set of the nucleic acids can be preselected for analysis. A first plurality of the nucleic acids of the first set that are present in the first biological sample can be captured. For each of the captured nucleic acids of the first plurality, an amount of that captured nucleic acid that is present in the first biological sample can be quantified and sequenced and based on the sequence of that captured nucleic acid, an origin of that captured nucleic acid can be identified. An indication can be output of the quantified amount and the identified origin of at least one captured nucleic acid that is present in the first biological sample.

    Abstract translation: 可以基于受试者经历的症状并且基于包括核酸的生物样品来诊断病症。 基于症状，可以预先选择第一组核酸进行分析。 可以捕获存在于第一生物样品中的第一组的第一组多个核酸。 对于第一多个捕获的核酸中的每一个，存在于第一生物样品中的所捕获的核酸的量可以进行定量和测序，并且基于捕获的核酸的序列，捕获的核酸的来源 可以识别。 可以输出存在于第一生物样品中的至少一种捕获的核酸的量化量和鉴定的来源。

    METHODS, SYSTEMS, COMPOSITIONS, KITS, APPARATUS AND COMPUTER-READABLE MEDIA FOR MOLECULAR TAGGING
    10.
    发明申请
    METHODS, SYSTEMS, COMPOSITIONS, KITS, APPARATUS AND COMPUTER-READABLE MEDIA FOR MOLECULAR TAGGING 审中-公开
    方法，系统，组合物，套件，用于分子标签的装置和计算机可读介质

    公开(公告)号：WO2016201142A1

    公开(公告)日：2016-12-15

    申请号：PCT/US2016/036763

    申请日：2016-06-09

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： MONGAN, Ann ,  CHIEN, Richard ,  BRINZA, Dumitru ,  BRAMLETT, Kelli

    IPC: C12Q1/68

    CPC classification number: C12Q1/6886 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2600/156 ,  C12Q2525/191 ,  C12Q2531/113 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2565/514

    Abstract: In some embodiments, the disclosure relates generally to methods, as well as related systems, compositions, kits, apparatuses and computer-readable media, comprising a multiplex molecular tagging procedure that employs a plurality of tags that are appended to a plurality of polynucleotides. The tags have characteristics, including a sequence, length and/or detectable moiety, or any other characteristic, that uniquely identifies the polynucleotide molecule to which it is appended, and permits tracking individual tagged molecules in a mixture of tagged molecules. For example, the tag having a unique tag sequence, can uniquely identify an individual polynucleotide to which it is appended, and distinguish the individual polynucleotide from other tagged polynucleotides in a mixture. In some embodiments, the multiplex molecular tagging procedure can be used for generating error-corrected sequencing data and for detecting a target polynucleotide which is present at low abundance in a nucleic acid sample.

    Abstract translation: 在一些实施方案中，本公开一般涉及方法以及相关系统，组合物，试剂盒，装置和计算机可读介质，其包含使用附加到多个多核苷酸的多个标签的多重分子标记过程。 标签具有特征，包括序列，长度和/或可检测部分，或唯一地识别其附着的多核苷酸分子的任何其它特征，并允许跟踪标记分子混合物中的单个标记分子。 例如，具有唯一标签序列的标签可以唯一地识别其所附着的单个多核苷酸，并将单个多核苷酸与混合物中的其他标记多核苷酸区分开。 在一些实施方案中，多重分子标记方法可用于产生错误校正的测序数据和用于检测以核酸样品中低丰度存在的靶多核苷酸。

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