公开(公告)号：WO2020104769A1

公开(公告)日：2020-05-28

申请号：PCT/GB2019/053168

申请日：2019-11-08

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： ISAAC, Tom ,  FRAYLING, Cameron

IPC: B01L3/00 ,  G01N35/08 ,  G01N33/48

Abstract: Devices, systems, and associated methods are provided for manipulating and/or determining one or more characteristics of cells contained within a biological sample. In particular a device and methods of use thereof are provided, the device comprising a sorting component configured to separate cell-containing microdroplets from empty ones into a population of cell-containing first microdroplets; a microdroplet manipulation component configured to manipulate the first microdroplets using real or virtual electrowetting electrodes, and an optical detection system configured to detect an optical signal from the microdroplets via the one or more detection windows.

公开(公告)号：WO2021009491A1

公开(公告)日：2021-01-21

申请号：PCT/GB2020/051665

申请日：2020-07-10

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： FRAYLING, Cameron ,  ISAAC, Thomas Henry ,  SOSNA, Maciej ,  ATHANASOPOULOU, Evangelia-Nefeli

IPC: B01L3/00 ,  C12M3/06

Abstract: A device for manipulating microdroplets, the device comprising a microfluidic chip adapted to receive and manipulate microdroplets dispersed in carrier fluid flowing along pathways on a surface of the chip, wherein the microdroplets are manipulated using an optically-mediated electrowetting (oEWOD) force; characterised in that the surface of the chip comprises a coating structure configured to allow controlled attachment and /or detachment of adherent cells contained within the microdroplets by application of the oEWOD force.

公开(公告)号：WO2022195281A1

公开(公告)日：2022-09-22

申请号：PCT/GB2022/050671

申请日：2022-03-16

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： ISAAC, Thomas Henry ,  HEAD, Emma ,  SOSNA, Maciej ,  DIECKMANN, Nele Marie Godette ,  MURZEAU, Claire Marie ,  ATHANASOPOULOU, Evangelia-Nefeli ,  WAEBER, Andrew ,  FRAYLING, Cameron ,  LOEFFEN, Paul

IPC: G01N33/50 ,  B01L3/00 ,  C12Q1/6881

Abstract: A method for selecting cells and/or part(s) of a cell(s) based on at least one characteristic in an EWOD or oEWOD device is provided. The method comprising: i. providing a test panel of microdroplets, comprising at least a medium or a medium and at least one cell; ii. providing at least one reporter panel of microdroplets, containing one or more reporter entities; iii. merging microdroplets of the test panel with microdroplets of the at least one reporter panel to form a panel of merged assay microdroplets; and iv. monitoring the panel of assay microdroplets using a detection system capable of detecting a change in said reporter entity based upon the presence of at least one characteristic, and v. selecting a subset of microdroplets from a panel on the basis of the change in the reporter entity, said subset containing the selected cells; wherein the test panel of microdroplets comprising at least medium is created by splitting a panel of microdroplets containing at least one cell and a medium, further creating a reference panel of microdroplets containing at least one cell; and/or further wherein the test panel of microdroplets comprising at least one cell are split into at least two panels of microdroplets before or after any of steps (i) to (v), said panels being: a test panel of microdroplets comprising at least medium or medium and at least one cell, suitable for merging with microdroplets of a reporter entity panel; and a reference panel of microdroplets containing at least one cell.

公开(公告)号：WO2021069911A1

公开(公告)日：2021-04-15

申请号：PCT/GB2020/052508

申请日：2020-10-09

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： FRAYLING, Cameron ,  ISAAC, Thomas Henry ,  WAEBER, Andreas Michael

IPC: C12Q1/18 ,  B01L3/00

Abstract: 27 ABSTRACT Provided is a method for determining an interaction between a medicament and a cell type, the method comprising: providing an array of first microdroplets, each containing a cell type derived from a 5 biological sample; providing an array of second microdroplets, each containing one or more medicaments at one or more predetermined concentrations, merging the array of first microdroplets and the array of second microdroplets to form an array of merged microdroplets;10 monitoring the characteristics of one or more cells in the merged microdroplets using an optical detection system configured to detect an interaction between a cell type and a medicament. Figure 1

公开(公告)号：WO2023281273A1

公开(公告)日：2023-01-12

申请号：PCT/GB2022/051763

申请日：2022-07-08

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： ISAAC, Thomas Henry ,  HEAD, Emma ,  PELLING, Aya ,  DEACON, William ,  FRAYLING, Cameron

IPC: B01L3/00 ,  B01F33/302

Abstract: A method of detecting the interactions between a biological entity and a molecule, the method comprising providing an array of first microdroplets into a microfluidic chip; wherein each microdroplet contains at least one bead and each bead having a bound photocleavable molecule; providing an array of second microdroplets into the microfluidic chip; wherein each microdroplet contains at least one biological entity; holding the entire first and second arrays of microdroplets; illuminating at least a subset of the first microdroplets containing at least one bead with an illumination source configured to photo-cleave the molecule; subsequently merging at least one subset of the first array of microdroplets with at least one subset of the second microdroplets to form an array of merged microdroplets; and detecting a change in an optical signal from the merged microdroplets using an optical system to indicate the interactions between the biological entity and the molecule. An apparatus for detecting the interaction between a biological entity and a molecule is also provided.

公开(公告)号：WO2021148804A2

公开(公告)日：2021-07-29

申请号：PCT/GB2021/050148

申请日：2021-01-22

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： BUSH, James ,  CONTERIO, Jasmin Kaur Chana ,  CUNHA, Pedro ,  DEACON, William Michael ,  FRAYLING, Cameron ,  ISAAC, Thomas Henry ,  TOPKAYA, Ibrahim Saygin

IPC: B01L3/00 ,  B01L2200/0673 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L3/502792

Abstract: The present invention provides methods and apparatus for manipulating and interrogating the contents of large numbers of microdroplets in parallel on a surface of a microfluidic chip. According to one aspect of the invention a method is provided for manipulating and inspecting microdroplets on a microfluidic chip by optically- mediated electrowetting (oEWOD), the method comprising forming, using a first optical assembly, a plurality of oEWOD traps on a surface of the chip and forming, using a second optical assembly, a second array of oEWOD traps on the surface of the chip, and making an adjustment to the first optical assembly whilst one or more of the microdroplets are held in place by second array of oEWOD traps. Apparatus comprising a microfluidic chip and first and second optical assemblies is also provided.