公开(公告)号：WO2022182865A8

公开(公告)日：2022-09-01

申请号：PCT/US2022/017692

申请日：2022-02-24

Applicant: 10X GENOMICS, INC.

Inventor： AKHREMICHEV, Ivan ,  CHEN, Lynna ,  SAUZADE, Martin

IPC: B01L3/00 ,  B01L2200/0673 ,  B01L2300/0681 ,  B01L2400/0487 ,  B01L3/502784

Abstract: The invention provides devices for forming droplets and concentrating droplets, and methods of their use. During operation of the devices, droplets are generated using two liquid phases. Once droplet formation is complete excess continuous phase is removed by the use of one or more pressure differentials.

公开(公告)号：WO2023283452A2

公开(公告)日：2023-01-12

申请号：PCT/US2022/036567

申请日：2022-07-08

Applicant: CEPHEID ,  GLASS, Jennifer Lynn ,  CHEN, Michael Quay ,  DORITY, John Benjamin ,  SIEGRIST, Jonathan Philip ,  WONG, Joanna

Inventor： GLASS, Jennifer Lynn ,  CHEN, Michael Quay ,  DORITY, John Benjamin ,  SIEGRIST, Jonathan Philip ,  WONG, Joanna

IPC: B01L3/00 ,  B01L7/00 ,  B01L2200/027 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/0893 ,  B01L2400/0406 ,  B01L2400/06 ,  B01L2400/0633 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502738 ,  B01L3/50851 ,  B01L7/52

Abstract: Reaction vessels, cartridges, devices and methods for facilitating high-level multiplexing are described herein. Such reaction vessels can include a planar frame defining a fluidic path between a first planar substrate and a second planar substrate, a fluidic interface is located at one end of the planar frame with a pair of fluidic ports, a well chamber and a pre-amplification chamber. Devices for spotting reagents in wells of high-level multiplexing reaction vessels and improved reagent solutions are also described herein.

公开(公告)号：WO2021250060A1

公开(公告)日：2021-12-16

申请号：PCT/EP2021/065393

申请日：2021-06-09

Applicant: MILTENYI BIOTEC B.V. & CO. KG

Inventor： ROCKENBACH, Alexander ,  MILTENYI, Stefan

IPC: B01L3/00 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/088 ,  B01L2400/043 ,  B01L2400/0633 ,  B01L3/502738 ,  B01L3/502761 ,  B01L3/502784

Abstract: A microfabricated droplet dispensing structure is described, which may include a plurality of MEMS microfluidic fluidic valves, configured to open and close a microfluidic channel. The opening and closing of the valve may separate a target particle and a bead from a sample stream, and direct these two particle into a single droplet formed at the edge of the substrate. At least one additional microfabricated valve may sort a barcoded bead, wherein the sorted bead is conjugated with the sorted cell to make a bead/cell complex. The complex may be enclosed in an aqueous droplet and the droplet dispensed into a stream of a second immiscible fluid.

公开(公告)号：WO2021222667A1

公开(公告)日：2021-11-04

申请号：PCT/US2021/030020

申请日：2021-04-29

Applicant: ABBOTT LABORATORIES ,  ARAI, Yoshiyuki ,  KOMORI, Tomotaka

Inventor： YOSHIMURA, Toru

IPC: B01L3/00 ,  G01N35/00 ,  B01L2200/0621 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0896 ,  B01L2400/043 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761

Abstract: Sample analysis systems and methods using assay surfaces, assay processing units (APUs), assay processing systems (APSs), and laboratory systems are disclosed. An assay surface includes a sample processing component comprising a plurality of regions, including at least one wash region and at least one storage region configured to hold a plurality of solid supports moveable through the regions under a magnetic force, and a detection component configured to receive the solid supports. An APU includes an assay surface receiving component, a magnetic element configured to generate a moveable magnetic field, and one or more processors configured to move the magnetic field. An APS includes one or more assay surfaces and an APU. A laboratory system includes one or more APSs and a controller for parallel processing. Sample processing and detection methods are disclosed with a reduced sample volume and/or shortened processing time and/or higher sensitivity.

公开(公告)号：WO2021055072A2

公开(公告)日：2021-03-25

申请号：PCT/US2020/040013

申请日：2020-06-27

Applicant: WISCONSIN ALUMNI RESEARCH FOUNDATION

Inventor： BEEBE, David J. ,  LANG, Joshua M. ,  JUANG, Duane S. ,  BERRY, Scott M. ,  RODEMS, Tamara S.

IPC: B01L3/00 ,  G01N33/543 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0864 ,  B01L2300/161 ,  B01L2400/043 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  G01N1/31 ,  G01N33/54326

Abstract: A method of sample preparation for streamlined multi-step assays is provided. The method includes the step of providing a microfluidic device including a reservoir defined by a surface configured to repel an aqueous solution. A dried reagent is provided on a portion of the surface and the reservoir is filled with an oil. A first droplet formed from the aqueous solution is positioned on the dried reagent so to pick-up and re-dissolve the dried reagent therein so as to expose the portion of the surface. In addition, a second droplet of an aqueous solution may be deposited on a hydrophilic spot patterned on the surface. A magnetic force may be configured to interact magnetically with the paramagnetic beads within the first droplet to move the droplet through the oil in the reservoir or to move the paramagnetic beads from the first droplet, through the oil, into the second droplet.

公开(公告)号：WO2023057642A1

公开(公告)日：2023-04-13

申请号：PCT/EP2022/077989

申请日：2022-10-07

Applicant: HOMEDICUS GMBH

Inventor： RIESTER, Markus ,  DIEBOLD, Michael

IPC: A61B5/15 ,  A61B5/151 ,  A61B5/157 ,  B01L3/00 ,  F16K99/00 ,  A61B5/150022 ,  A61B5/150221 ,  A61B5/150251 ,  A61B5/150358 ,  A61B5/150389 ,  A61B5/150419 ,  A61B5/15107 ,  B01L2200/0673 ,  B01L2300/0654 ,  B01L2300/0803 ,  B01L2400/0406 ,  B01L2400/0457 ,  B01L2400/0688 ,  B01L3/502738 ,  F16K99/0021

Abstract: The invention relates to a liquid sampling device (100) comprising a support structure (106) having a liquid reception interface (130) that is in fluid communication with a reservoir chamber (134) and a microfluidic valve (136), the microfluidic valve (136) being connected to a measuring chamber (138). The liquid reception interface (130), the reservoir chamber (134) and the microfluidic valve (136) are configured to allow liquid entering the liquid reception interface to flow into the reservoir chamber (134) while being prevented from passing the microfluidic valve (136) unless a predetermined static pressure difference over the microfluidic valve (136) is exceeded. The microfluidic valve (136) is a passive valve without moving parts. Passing of liquid through the microfluidic valve is controlled by a static pressure difference overthe microfluidic valve (136). The reservoir chamber (134) is configured and arranged to cause high enough a static pressure at an entrance side of the microfluidic valve (136) that causes a flow of liquid through the microfluidic valve (136) into the measuring chamber (138) once the reservoir chamber (134) is completely filled.

公开(公告)号：WO2022211637A1

公开(公告)日：2022-10-06

申请号：PCT/NL2022/050186

申请日：2022-04-02

Applicant: EMULTECH B.V.

Inventor： VAN STIPHOUT, Petrus Casper Martinus ,  DERKS, Tom ,  VAN DER WEIJDEN, Ferdi Raymon

IPC: B01L3/00 ,  G01N35/08 ,  B01F23/41 ,  B01F33/3011 ,  B01L2200/0673 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/086 ,  B01L3/502746 ,  B01L3/502784

Abstract: The invention relates to a microfluidic chip comprising at least two units for droplet formation, each unit comprising a first supply channel for supplying a first phase, a second supply channel for supplying a second phase and a discharge channel for discharging a product phase, wherein the first and second supply channel converge at a junction to the discharge channel. The hydraulic resistance of the supply channels is higher than that of the discharge channel, so that there is a better flow at the junction, yielding droplets of higher uniformity. In a preferred embodiment, there is a manifold that feeds all the units for droplet formation in a parallel fashion, wherein the manifold has at least a ten times lower hydraulic resistance than the supply channels in the units for droplet formation. This results in droplets that have an even higher uniformity. Another advantage, especially with higher numbers of units for droplet formation in the chip, is that there is less disturbance of liquid flow by gas bubbles and less inactivity of channels when starting a process in the microfluidic chip.

公开(公告)号：WO2021239871A2

公开(公告)日：2021-12-02

申请号：PCT/EP2021/064200

申请日：2021-05-27

Applicant: UNIVERSITEIT LEIDEN

Inventor： HANKEMEIER, Thomas ,  MIGGIELS, Anthonius Lambertus Wilhelmus ,  WOUTERS, Bert

IPC: B01L3/00 ,  G01R33/30 ,  G01N35/10 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/0838 ,  B01L2300/165 ,  B01L3/502707 ,  B01L3/502784 ,  G01N35/1095 ,  G01R33/307 ,  G01R33/46

Abstract: A fluidic assembly comprising a fluid analysis apparatus (30), the fluid analysis apparatus (30) comprising: a fluid measurement device (34); a fluidic device including a flow cell (36) arranged in a measurement region of the fluid measurement device (34), the flow cell (36) constructed of at least one first fluoropolymer material, the flow cell (36) including a channel, the channel containing a sample segment (52) that is carried in a fluorinated fluid carrier (54), wherein the sample segment (52) and fluorinated fluid carrier (54) are immiscible.

公开(公告)号：WO2022212674A1

公开(公告)日：2022-10-06

申请号：PCT/US2022/022781

申请日：2022-03-31

Applicant: CHEMRING SENSORS & ELECTRONIC SYSTEMS, INC.

Inventor： SATTERLY, Neal G. ,  PFEUFFER, Kevin P.

IPC: B01L3/00 ,  C12Q1/6844 ,  B01L2200/0673 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/1805 ,  B01L2400/0427 ,  B01L3/502784 ,  B01L7/52 ,  C12Q1/6816 ,  C12Q1/686 ,  C12Q1/6876 ,  C12Q2563/107

Abstract: A device, method, and non-transitory computer readable medium storing instructions for nucleic acid identification of material in a sample includes a microfluidics system, including a movable cartridge, and a heat source. The movable cartridge assembly includes at least one target-specific set of reagent components and a set of RCA-LAMP reaction components deposited on the surface. Moreover, the at least one target-specific set of reagent components includes at least one target specific padlock probe reagent component. The set of RCA‑LAMP reaction components includes: at least one polymerase buffer component, at least one polymerase enzyme with strand displacement activity, a betaine additive, a TETRONIC additive, a sequence-specific probe, dNTPs, and a primer mix. The primer mix includes both a forward inside primer and a backward inside primer specific to a backbone of the at least one target specific padlock probe reagent component.

公开(公告)号：WO2021252810A1

公开(公告)日：2021-12-16

申请号：PCT/US2021/036885

申请日：2021-06-10

Applicant: CHECKABLE MEDICAL INCORPORATED

Inventor： VAINIKKA, Caleb

IPC: G01N33/558 ,  G01N31/22 ,  G01N33/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/047 ,  B01L2400/0481 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/5029 ,  B01L3/523

Abstract: A method of identifying the presence of an analyte may include providing an in vitro test device and a test swab; obtaining a sample using the test swab; transitioning the locking member from the first configuration to the second configuration; advancing the plunger into the housing to pierce the one or more reagent pouches in the reagent region to cause reagent therein to be released and mix; inserting the test swab with obtained sample into the interior of the plunger; rotating the in vitro test device and disposing it on its plunger base; and determining whether the analyte is present. The in vitro test device may include a plunger, a housing, one or more reagent pouches disposed in the housing, a lateral flow test strip, and a locking member having configurations that either allow or prevent reagent in the one or more reagent pouches from being released.