公开(公告)号：WO2021245635A1

公开(公告)日：2021-12-09

申请号：PCT/IB2021/054981

申请日：2021-06-07

Applicant: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)

Inventor： THIRIET, Pierre-Emmanuel ,  GUIDUCCI, Carlotta ,  PORRO, Gloria

IPC: B01L3/00 ,  B03C5/00 ,  G01N1/00 ,  G01N27/00 ,  B03C5/02 ,  G01N1/40 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0848 ,  B01L2400/0424 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502761 ,  B03C2201/24 ,  B03C2201/26 ,  B03C5/005 ,  B03C5/026 ,  G01N2001/4038

Abstract: A dielectrophoretic detection device comprising a chip, with a flow channel having at least one inlet and one outlet, and at least a detection area configured to detect analytes trapped on functionalised beads flowing within said flow channel, a first and a second electrode assembly shaped as rows of parallel pillars extending over said height of the flow channel, configured to generate under an appropriate electric tension therebetween an electric field so as to form an electrical barrier, preventing said beads to cross said barrier and drawing said beads to said detection area by dielectrophoretic forces, where they are clustered and concentrated. The device may be provided with multiple rows of parallel pillars of electrode assemblies extending over the height of the flow channel, forming multiple concentration lines. The flow channel may be further provided with further rows of parallel pillars of electrode assemblies crossing said flow channel in transverse direction, forming further incubation lines.

公开(公告)号：WO2021111133A2

公开(公告)日：2021-06-10

申请号：PCT/GB2020/053096

申请日：2020-12-03

Applicant: OXFORD NANOPORE TECHNOLOGIES LIMITED

Inventor： BROWN, Clive Gavin ,  ORTIZ BAHAMON, Pedro Miguel ,  BOWEN, Rebecca Victoria

IPC: B01L3/00 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0819 ,  B01L2300/0829 ,  B01L2300/0864 ,  B01L2300/0883 ,  B01L2300/0896 ,  B01L2300/1805 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/0481 ,  B01L2400/06 ,  B01L2400/0683 ,  B01L2400/086 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502792

Abstract: The invention resides in a microfluidic device for analysing a test liquid. The device has a preparation portion between an inlet and an upstream portion of the device, wherein said upstream portion is configured with a sensing chamber for housing a well array and for receiving via the inlet a test liquid to be sensed, and said preparation portion is configured having: a reagent port configured to receive a reagent for interacting with a test liquid to be sensed within the upstream portion; and/or an actuator, operably configured to induce a test liquid from the inlet towards the upstream portion. Between the inlet and respective outlet lies a sole channel. The channel can extend parallel to the substrate, preferably along its entire length. The preparation portion can be configured in a first part, and the upstream portion can be configured in a second part, wherein said first part and second part are connectable such that a sample received and processed in the first part can be passed to the second part for analysis.

公开(公告)号：WO2023275503A1

公开(公告)日：2023-01-05

申请号：PCT/GB2021/051658

申请日：2021-06-30

Applicant: QUANTUMDX GROUP LIMITED

Inventor： BOADA ORTIZ, Eduardo ,  SCHMID, Lothar ,  MURTON, Heather

IPC: B01L3/00 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/08 ,  B01L2400/0424 ,  B01L2400/084 ,  B01L3/50273 ,  B01L3/502746 ,  B01L3/502761

Abstract: Disclosed herein is a microfluidic device for concentrating target particles in a fluid sample using dielectrophoresis (DEP). The microfluidic device comprises an inlet chamber comprising a fluid inlet for receiving a fluid sample, an outlet chamber comprising a fluid outlet for discharging the fluid sample, and a plurality of DEP channels. Each DEP channel is fluidically connected to the inlet chamber and to the outlet chamber such a fluid path from the fluid inlet to the fluid outlet is provided through each of the DEP channels, wherein the microfluidic device is configured such that each of the fluid paths has substantially the same fluid resistance.

公开(公告)号：WO2021211754A2

公开(公告)日：2021-10-21

申请号：PCT/US2021/027347

申请日：2021-04-14

Applicant: QUANTERIX CORPORATION

Inventor： DUFFY, David, C. ,  COLLIER, Nicholas ,  KAN, Cheuk, W. ,  TOBOS, Carmen, I. ,  RISSIN, David, M. ,  WIENER, Alexander, D. ,  MEYER, Ray ,  WARWICK, Christopher ,  MILLINGTON, Roger ,  SVANCARA, Danielle, M.

IPC: B01L3/00 ,  G01N1/40 ,  G01N35/00 ,  G01N35/08 ,  B01L2200/0668 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0877 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/043 ,  B01L3/502761 ,  B01L3/502784 ,  G01N15/0612 ,  G01N15/1456 ,  G01N15/1484 ,  G01N2015/03 ,  G01N2035/0441 ,  G01N35/0098

Abstract: Methods and systems for capture object-based assays, including for determining a measure of the concentration of an analyte molecule or particle in a fluid sample, are described. The methods and systems may relate to high sensitivity detection of analytes, sometimes using assay conditions and sample handling that result in the capture and detection of a high percentage of the analyte molecules or particles in a fluid sample using relatively few capture objects. Apparatuses and methods for immobilizing capture objects with respect to assay sites, in some instances with unexpectedly high efficiencies are also described. Some such apparatuses involve the use of force fields and fluid meniscus forces, alone or in combination, to facilitate or improve capture object immobilization. Also described are techniques for utilizing a relatively high percentage of capture objects in an assay sample, such as by using disclosed sample washing techniques, imaging systems, and analysis procedures that can reduce capture object loss.

公开(公告)号：WO2021194844A2

公开(公告)日：2021-09-30

申请号：PCT/US2021/022968

申请日：2021-03-18

Applicant: STRIKE PHOTONICS, INC.

Inventor： CAROTHERS, Daniel

IPC: G01N21/63 ,  B01L3/00 ,  B01L2200/0684 ,  B01L2300/023 ,  B01L2300/0645 ,  B01L2300/0825 ,  B01L2300/0883 ,  B01L2300/0896 ,  B01L2300/168 ,  B01L2400/0406 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/0487 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502761 ,  B82Y20/00 ,  G01B9/02058 ,  G02B2006/12061 ,  G02B2006/12078 ,  G02B2006/12138 ,  G02B2006/12159 ,  G02B2006/12176 ,  G02B6/12 ,  G02B6/12004 ,  G02B6/42

Abstract: This disclosure provides photonic integrated chip that has an optical waveguide located on a photonic circuit substrate that includes a photonic circuit that is optically coupled to the waveguide. A microfluidic channel is in a silicon substrate and is attached to the photonic circuit substrate. The microfluidic channel is positioned over the optical waveguide such that its side surfaces and an outermost surface extend into the microfluidic channel. The microfluidic channel extends along a length of the optical waveguide, and nanoparticles are located on or adjacent the optical waveguide located within the microfluidic channel.

公开(公告)号：WO2021148804A2

公开(公告)日：2021-07-29

申请号：PCT/GB2021/050148

申请日：2021-01-22

Applicant: LIGHTCAST DISCOVERY LTD

Inventor： BUSH, James ,  CONTERIO, Jasmin Kaur Chana ,  CUNHA, Pedro ,  DEACON, William Michael ,  FRAYLING, Cameron ,  ISAAC, Thomas Henry ,  TOPKAYA, Ibrahim Saygin

IPC: B01L3/00 ,  B01L2200/0673 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L3/502792

Abstract: The present invention provides methods and apparatus for manipulating and interrogating the contents of large numbers of microdroplets in parallel on a surface of a microfluidic chip. According to one aspect of the invention a method is provided for manipulating and inspecting microdroplets on a microfluidic chip by optically- mediated electrowetting (oEWOD), the method comprising forming, using a first optical assembly, a plurality of oEWOD traps on a surface of the chip and forming, using a second optical assembly, a second array of oEWOD traps on the surface of the chip, and making an adjustment to the first optical assembly whilst one or more of the microdroplets are held in place by second array of oEWOD traps. Apparatus comprising a microfluidic chip and first and second optical assemblies is also provided.