公开(公告)号：WO2007098379A3

公开(公告)日：2008-11-20

申请号：PCT/US2007062270

申请日：2007-02-16

Applicant: LUMIGEN INC ,  AKHAVAN-TAFTI HASHEM ,  DE SILVA RENUKA ,  EICKHOLT ROBERT A ,  MAZELIS MICHAEL E ,  XIE WENHUA ,  HANDLEY RICHARD S ,  BRAY MONICA A ,  MASTRONARDI MICHELLE L ,  O'CONNOR ELIZABETH A ,  SIRIPURAPU SARADA

Inventor： AKHAVAN-TAFTI HASHEM ,  DE SILVA RENUKA ,  EICKHOLT ROBERT A ,  MAZELIS MICHAEL E ,  XIE WENHUA ,  HANDLEY RICHARD S ,  BRAY MONICA A ,  MASTRONARDI MICHELLE L ,  O'CONNOR ELIZABETH A ,  SIRIPURAPU SARADA

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC classification number: C12Q1/6806

Abstract: Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.

Abstract translation: 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。

公开(公告)号：WO2006019388A2

公开(公告)日：2006-02-23

申请号：PCT/US2004039762

申请日：2004-12-13

Applicant: LUMIGEN INC ,  AKHAVAN-TAFTI HASHEM ,  DESILVA RENUKA ,  EICKHOLT ROBERT A ,  GUNDLACH WILLIAM C ,  HANDLEY RICHARD S ,  LAUWERS KENNETH S ,  SANDISON MARK D ,  XIE WENHUA

Inventor： AKHAVAN-TAFTI HASHEM ,  DESILVA RENUKA ,  EICKHOLT ROBERT A ,  GUNDLACH WILLIAM C ,  HANDLEY RICHARD S ,  LAUWERS KENNETH S ,  SANDISON MARK D ,  XIE WENHUA

IPC: C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6823

Abstract: Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or micowells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

Abstract translation: 公开了用于结合核酸的固相材料及其使用方法。 该材料具有可切割的接头部分，其可以被切割以释放结合的核酸。 所述固相材料包含固体载体部分，所述固体载体部分包含选自二氧化硅，玻璃，不溶性合成聚合物和不可溶合的多糖的基质，所述多糖附着有用于吸引和结合核酸的核酸结合部分，所述核酸结合部分（NAB） 通过可裂解的接头部分与固体支持部分连接。 优选的核酸结合部分包含三元或四元鎓基团。 材料可以是微粒，纤维，珠子，膜，试管或微孔的形式，并且可以进一步包含磁芯部分。 公开了使用可切割固体支持物结合核酸的方法，以及它们在分离或纯化核酸的方法中的用途。

公开(公告)号：WO2004074810A3

公开(公告)日：2005-08-25

申请号：PCT/US2004002020

申请日：2004-02-17

Applicant: LUMIGEN INC ,  AKHAVAN-TAFTI HASHEM ,  EICKHOLT ROBERT A ,  LAUWERS KENNETH S ,  HANDLEY RICHARD S

Inventor： AKHAVAN-TAFTI HASHEM ,  EICKHOLT ROBERT A ,  LAUWERS KENNETH S ,  HANDLEY RICHARD S

IPC: C07D305/00 ,  C07D321/00 ,  C07F5/02 ,  C12N9/96 ,  C12Q1/26 ,  G01N20060101 ,  G01N21/76 ,  G01N33/53 ,  G01N33/533 ,  G01N33/534

CPC classification number: C07F5/025 ,  G01N33/533 ,  Y10S436/904 ,  Y10T436/141111 ,  Y10T436/142222 ,  Y10T436/206664

Abstract: Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula (I) is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig - OH or Sig -O>-

Abstract translation: 公开了用于检测过氧化氢源的方法和化合物，其中式（I）的信号化化合物与过氧化物反应。 Sig是非聚合有机基团，B是硼原子，并且每个R独立地选自氢，烷基和芳基，并且可以作为形成环或芳环的直链或支链亚烷基链连接在一起。 通过测量颜色，吸光度，荧光，化学发光或生物发光产生和检测式Sig-OH​​或Sig -O> - 的可检测产物化合物。 信号化合物本身不具有可检测的性质，或仅仅是非常弱的程度。 该方法可用作过氧化物或产生过氧化物的酶的测定中的可检测信号，以及使用酶标记的特异性结合对的测定中。

公开(公告)号：WO03027004A3

公开(公告)日：2003-07-03

申请号：PCT/US0225946

申请日：2002-08-27

Applicant: LUMIGEN INC

Inventor： AKHAVAN-TAFTI HASHEM ,  HANDLEY RICHARD S ,  SANDISON MARK D ,  LARKIN RANDALL K

IPC: C09K3/00 ,  B01J31/02 ,  B01J31/06 ,  B01J35/00 ,  C08F8/00 ,  C08F8/44 ,  C08C19/22 ,  C08C19/24

CPC classification number: C08F8/44 ,  B01J31/0234 ,  B01J31/06 ,  B01J35/004 ,  C08F8/18 ,  C08F8/40 ,  C08F212/08

Abstract: Improved polymer-immobilized photosensitizer are disclosed as well as methods of preparing and using them. The polymer-immobilized photosensitizers comprise a cross-linked polymer backbone, a plurality of cationic ammonium or phosphonium groups covalently bound to the polymer backbone and an immobilized photosensitizer. The average total number of carbon atoms in the ammonium or phosphonium group is at least four and preferably at least 12. The photosensitizer can be either covalently or ionically bound to the polymer. Polymer-supported photosensitizers of the invention are unexpectedly superior in catalyzing the photosensitized oxidation of compounds containing carbon-carbon double bonds.

Abstract translation: 公开了改进的聚合物固定的光敏剂以及制备和使用它们的方法。 聚合物固定的光敏剂包含交联的聚合物主链，与聚合物主链共价结合的多个阳离子铵基或鏻基和固定的光敏剂。 铵或鏻基团中碳原子的平均总数为至少4，优选至少12.光敏剂可以共价或离子键合到聚合物上。 本发明的聚合物支持的光敏剂在催化含有碳 - 碳双键的化合物的光敏氧化方面意想不到地优异。