公开(公告)号：WO2006036246A3

公开(公告)日：2006-04-06

申请号：PCT/US2005/023608

申请日：2005-07-01

Applicant: LUMIGEN, INC. ,  AKHAVAN-TAFTI, Hashem

Inventor： AKHAVAN-TAFTI, Hashem

IPC: C12Q1/68 ,  C07H21/00

Abstract: Methods of isolating nucleic acids from samples of cellular material are disclosed which use solid phase binding materials and which avoid the use of a lysis solution. The use of the solid phase binding materials unexpectedly allow the nucleic acid content of cells to be freed and captured directly and in one step. The new methods represent a significant simplification over existing methods. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.

公开(公告)号：WO2006019388A3

公开(公告)日：2006-02-23

申请号：PCT/US2004/039762

申请日：2004-12-13

Applicant: LUMIGEN, INC. ,  AKHAVAN-TAFTI, Hashem ,  DESILVA, Renuka ,  EICKHOLT, Robert, A. ,  GUNDLACH, William, C. ,  HANDLEY, Richard, S. ,  LAUWERS, Kenneth, S. ,  SANDISON, Mark, D. ,  XIE, Wenhua

Inventor： AKHAVAN-TAFTI, Hashem ,  DESILVA, Renuka ,  EICKHOLT, Robert, A. ,  GUNDLACH, William, C. ,  HANDLEY, Richard, S. ,  LAUWERS, Kenneth, S. ,  SANDISON, Mark, D. ,  XIE, Wenhua

IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04

Abstract: Solid phase materials for binding nucleic acids and methods of their use are disclosed. The materials feature a cleavable linker portion which can be cleaved to release bound nucleic acids. The solid phase materials comprise a solid support portion comprising a matrix selected from silica, glass, insoluble synthetic polymers, and insoluble polysaccharides to which is attached a nucleic acid binding portion for attracting and binding nucleic acids, the nucleic acid binding portion (NAB) being linked by a cleavable linker portion to the solid support portion. Preferred nucleic acid binding portions comprise a ternary or quaternary onium group. The materials can be in the form of microparticles, fibers, beads, membranes, test tubes or micowells and can further comprise a magnetic core portion. Methods of binding nucleic acids using the cleavable solid supports are disclosed as are their use in methods of isolating or purifying nucleic acids.

公开(公告)号：WO1994010337A1

公开(公告)日：1994-05-11

申请号：PCT/US1993009740

申请日：1993-10-12

Applicant: LUMIGEN, INC.

Inventor： LUMIGEN, INC. ,  AKHAVAN-TAFTI, M., Hashem

IPC: C12Q01/00

CPC classification number: G01N33/581 ,  C12Q1/28 ,  C12Q1/6816 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S436/819

Abstract: An assay method, compositions and test kits using a hydroxyaryl cyclic diacylhydrazide is described. A hydrogen peroxide and peroxidase enzyme. The preferred compositions incorporate enhancer compounds and a chelating agent which suppresses light production prior to addition of a peroxidase enzyme. The assay method can test for a peroxidase enzyme, a peroxide or can be used in immunoassays and probe assays.

Abstract translation: 描述了使用羟基芳基环二酰基酰肼的测定方法，组合物和测试试剂盒。 过氧化氢和过氧化物酶。 优选的组合物掺入增强剂化合物和在加入过氧化物酶之前抑制光产生的螯合剂。 测定方法可以测试过氧化物酶，过氧化物或可用于免疫测定和探针测定。

公开(公告)号：WO2003104447A1

公开(公告)日：2003-12-18

申请号：PCT/US2003/013410

申请日：2003-05-15

Applicant: LUMIGEN, INC.

Inventor： AKHAVAN-TAFTI, Hashem ,  DE SILVA, Renuka ,  SANDISON, Mark, D. ,  HANDLEY, Richard, S.

IPC: C12N9/96

CPC classification number: C12Q1/28 ,  G01N33/533 ,  Y10S435/968

Abstract: Methods of producing fluorescence from fluorogenic substrates reactive with a peroxidase enzyme are disclosed. Use of the methods in assays for peroxidase enzymes, peroxidase-labeled analytes are provided. Fluorogenic compounds, compositions and kits for reaction with peroxidase enzymes are described. Two modes of producing fluorescent compounds are described.

Abstract translation: 公开了从与过氧化物酶反应的荧光底物产生荧光的方法。 提供了在过氧化物酶，过氧化物酶标记的分析物的测定中使用这些方法。 描述了与过氧化物酶反应的荧光化合物，组合物和试剂盒。 描述了产生荧光化合物的两种模式。

公开(公告)号：WO9954503B1

公开(公告)日：1999-11-25

申请号：PCT/US9906531

申请日：1999-04-16

Applicant: LUMIGEN INC

Inventor： AKHAVAN-TAFTI HASHEM

IPC: G01N33/532 ,  C12Q1/28 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/68 ,  G01N33/535 ,  G01N33/58 ,  G01N33/53 ,  G01N33/545 ,  G01N33/552

CPC classification number: G01N33/582 ,  C12Q1/28 ,  Y10S435/973

Abstract: A method for sequential chemiluminescent detection of two differently labeled analytes on a single blot is described. In the method, a uniquely labeled DNA is detected with a horseradish peroxidase (HRP) substrate followed by the detection of another uniquely labeled DNA with a second different enzyme substrate which also inhibits the chemiluminescence generated by HRP. The sequential detection method described herein eliminates the need to strip and reprobe Southern, Northern and Western blots. Potential applications of this method include forensic DNA fingerprinting where more than one probe is used for probing a Southern blot, multiplex DNA sequencing of more than one template, detection of gene rearrangements, mutations and gene linkage.

Abstract translation: 描述了在单个印迹上顺序化学发光检测两种不同标记的分析物的方法。 在该方法中，用辣根过氧化物酶（HRP）底物检测到唯一标记的DNA，随后用也抑制由HRP产生的化学发光的第二不同酶底物检测另一个唯一标记的DNA。 本文所述的顺序检测方法消除了剥离和重新覆盖南部，北部和西部印迹的需要。 该方法的潜在应用包括法医DNA指纹图谱，其中使用多于一个探针来探测Southern印迹，多个DNA测序多个模板，检测基因重排，突变和基因连锁。

公开(公告)号：WO1996007911A1

公开(公告)日：1996-03-14

申请号：PCT/US1995010952

申请日：1995-08-30

Applicant: LUMIGEN, INC.

Inventor： LUMIGEN, INC. ,  AKHAVAN-TAFTI, Hashem ,  ARGHAVANI, Zahra ,  DeSILVA, Renuka

IPC: G01N33/535

CPC classification number: C07D219/02 ,  C07D219/04 ,  C07D219/06 ,  C09B15/00 ,  C12Q1/28 ,  G01N33/581 ,  G01N33/582 ,  Y10S435/966 ,  Y10S435/968 ,  Y10S435/975

Abstract: A chemiluminescent assay method, compositions and kits are described which use a protected phenolic enhancer compound which is deprotected by a hydrolytic enzyme and then enhances a chemiluminescent reaction. The reaction involves an acridan compound, preferably a derivative of an N-alkyl-acridan-9-carboxylic acid, which is activated to produce light by a peroxide compound and a peroxidase enzyme in the presence of the deprotected enhancer. The result is enhanced generation of light from the reaction. The hydrolytic enzyme is present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the hydrolytic enzyme is bound to a reporter molecule.

Abstract translation: 描述了化学发光测定方法，组合物和试剂盒，其使用被水解酶去保护的受保护的酚类增强剂化合物，然后增强化学发光反应。 该反应涉及吖啶化合物，优选N-烷基 - 吖啶-9-羧酸的衍生物，其在去保护的增强子存在下被过氧化物和过氧化物酶活化以产生光。 结果是增强了反应产生的光。 水解酶在免疫测定，DNA探针测定或其他测定中单独存在或连接到特异性结合对的成员，其中水解酶与报道分子结合。

公开(公告)号：WO1995028495A1

公开(公告)日：1995-10-26

申请号：PCT/US1995002918

申请日：1995-03-08

Applicant: LUMIGEN, INC.

Inventor： LUMIGEN, INC. ,  AKHAVAN-TAFTI, Hashem ,  DESILVA, Renuka ,  ARGHAVANI, Zahra

IPC: C12Q01/28

CPC classification number: C07D219/06 ,  C07D219/02 ,  C07D219/04 ,  C12Q1/28 ,  Y10S435/81 ,  Y10S435/968

Abstract: Aryl N-alkylacridanthiocarboxylate compounds are used with peroxide and peroxidase to generate chemiluminescence. The compounds are used as a substrate in assays for various analytes. The figure shows a graph for a comparison of the light emission profiles from a reagent containing an aryl N-alkylacridanthiocarboxylate compound.

Abstract translation: 硝基N-烷基酰基羧酸盐化合物与过氧化物和过氧化物酶一起使用以产生化学发光。 该化合物用作各种分析物测定中的底物。 该图示出了用于比较来自含有N-烷基酰基羧酸芳基酯化合物的试剂的发光谱的曲线图。

公开(公告)号：WO1995023971A1

公开(公告)日：1995-09-08

申请号：PCT/US1995002568

申请日：1995-03-01

Applicant: LUMIGEN, INC.

Inventor： LUMIGEN, INC. ,  AKHAVAN-TAFTI, Hashem ,  DESILVA, Renuka ,  ARGHAVANI, Zahra ,  SCHOENFELNER, Barry, A.

IPC: G01N33/53

CPC classification number: G01N33/533 ,  C07D219/02 ,  C07D219/04 ,  C07D219/06 ,  C12Q1/28 ,  Y10S435/968

Abstract: Acridans which are reactable with a peroxidase and peroxide. The acridans are characterized by having an aromatic leaving group ArO which is a di- or polyhalosubstituted phenoxy group. The compounds are useful in assays where one member of a binding pair is linked to the peroxidase and for detecting the peroxidase. The method can also be used to detect hydrogen peroxide.

Abstract translation: 可与过氧化物酶和过氧化物反应的吖啶。 吖啶的特征在于具有芳族离去基团ArO，其为二取代或多取代的苯氧基。 该化合物可用于其中一个结合对成员与过氧化物酶连接并检测过氧化物酶的测定中。 该方法也可用于检测过氧化氢。

公开(公告)号：WO2007098379A3

公开(公告)日：2008-11-20

申请号：PCT/US2007062270

申请日：2007-02-16

Applicant: LUMIGEN INC ,  AKHAVAN-TAFTI HASHEM ,  DE SILVA RENUKA ,  EICKHOLT ROBERT A ,  MAZELIS MICHAEL E ,  XIE WENHUA ,  HANDLEY RICHARD S ,  BRAY MONICA A ,  MASTRONARDI MICHELLE L ,  O'CONNOR ELIZABETH A ,  SIRIPURAPU SARADA

Inventor： AKHAVAN-TAFTI HASHEM ,  DE SILVA RENUKA ,  EICKHOLT ROBERT A ,  MAZELIS MICHAEL E ,  XIE WENHUA ,  HANDLEY RICHARD S ,  BRAY MONICA A ,  MASTRONARDI MICHELLE L ,  O'CONNOR ELIZABETH A ,  SIRIPURAPU SARADA

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC classification number: C12Q1/6806

Abstract: Methods and materials are disclosed for rapid and simple extraction and isolation of nucleic acids, particularly RNA, from a biological sample involving the use of an alkaline reagent followed by an acidic solution and a solid phase binding material that has the ability to liberate nucleic acids from biological samples, including whole blood, without first performing any preliminary lysis to disrupt cells or viruses. No detergents or chaotropic substances for lysing cells or viruses are needed or used. Viral, bacterial and mammalian genomic RNA can be obtained using the method of the invention. RNA obtained by the present method is suitable for use in downstream processes such as RT-PCR.

Abstract translation: 公开的方法和材料用于从涉及使用碱性试剂的酸性溶液和固相结合材料的生物样品中快速和简单地提取和分离核酸，特别是RNA，所述固体相结合材料具有将核酸从 生物样品，包括全血，而不首先进行任何初步裂解以破坏细胞或病毒。 不需要或使用用于裂解细胞或病毒的洗涤剂或离液序列物质。 可以使用本发明的方法获得病毒，细菌和哺乳动物基因组RNA。 通过本方法获得的RNA适用于下游过程如RT-PCR。

公开(公告)号：WO2006036243A3

公开(公告)日：2007-07-26

申请号：PCT/US2005023519

申请日：2005-07-05

Applicant: LUMIGEN INC ,  AKHAVAN-TAFTI HASHEM

Inventor： AKHAVAN-TAFTI HASHEM

IPC: C12Q1/68 ,  C07H21/00 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12N15/1006 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q2565/518 ,  C12Q2563/143 ,  C12Q2527/113

Abstract: Methods of isolating nucleic acids from samples of biological or cellular material are disclosed which use solid phase binding materials and which avoid the use of any lysis solution or coating. The use of the solid phase binding materials unexpectedly allow the nucleic acid content of cells to be freed and captured directly and in one step. the new methods represent a significant simplification over existing methods. Nucleic acids can be captured and released in a form suitable for downstream processing in under five minutes. Preferred solid phase materials for use with the methods and compositions of the invention comprise a quaternary onium nucleic acid binding portion.

Abstract translation: 公开了从生物或细胞材料样品分离核酸的方法，其使用固相结合材料，并且避免使用任何裂解溶液或涂层。 固相结合材料的使用意外地允许细胞的核酸含量被直接释放并且在一个步骤中捕获。 新方法代表了对现有方法的显着简化。 核酸可以在五分钟内以适合下游处理的形式被捕获和释放。 与本发明的方法和组合物一起使用的优选固相材料包含季鎓核酸结合部分。