公开(公告)号：WO2023060310A1

公开(公告)日：2023-04-20

申请号：PCT/AU2022/051229

申请日：2022-10-13

Applicant: MEAT & LIVESTOCK AUSTRALIA LIMITED

Inventor： GIBB, Zamira ,  KLEIN, Erin ,  AITKEN, Robert John

IPC: A01N1/02 ,  A61K35/52 ,  A61K47/02 ,  A61K47/10 ,  A61K47/12 ,  A61K47/18 ,  A61K47/22 ,  A61K47/26 ,  A61K9/08 ,  A61P15/08 ,  C12N5/076

Abstract: There is provided a sperm extender medium for prolonging the fertility of bovine spermatozoa during liquid storage of the spermatozoa. The medium comprises a monosaccharide energy source for the spermatozoa. The one or more α or β amino acid osmolytes are selected from mono-amino carboxylic and mono-amino sulfonic acids, each independently having an R side group selected from the group consisting of H and non-polar, neutral open aliphatic chains excluding branched C3 and longer aliphatic chains. The medium also comprises one or more antioxidants and physiologically acceptable inorganic salts for maintaining sperm viability, and is buffered for maintaining pH in a range of from about 7.0 to about 8.5. Further, in embodiments, the medium has a concentration of sodium ions of less than about 95 mM, and an osmolarity in a range of from about 290 mOsm/L to about 400 mOsm/L. There are also provided preparations comprising the spermatozoa in the medium, methods for preparing the medium and methods for fertilisation of an ovum comprising contacting the ovum with spermatozoa administered in the sperm extender medium.