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    METHODS FOR SPERMATOGONIAL CULTURE
    1.
    发明申请
    METHODS FOR SPERMATOGONIAL CULTURE 审中-公开

    公开(公告)号：WO2022026999A1

    公开(公告)日：2022-02-03

    申请号：PCT/US2021/070967

    申请日：2021-07-27

    Applicant: WASHINGTON STATE UNIVERSITY

    Inventor： OATLEY, John Michael ,  OATLEY, Melissa Joan

    IPC: C12N5/076 ,  A61K35/52

    Abstract: This disclosure provides improved method, kits, and systems and for isolating and enriching spermatogonial stem cells (SSCs) from livestock animal testicular tissue. In one aspect, the disclosure provides a method for enriching SSCs from a population of testis-derived cells containing at least one SSC, where the method comprises contacting the population of testis-derived cells to a culture media comprising, or that is preconditioned with, endothelial feeder cells, and maintaining culture conditions suitable for SSC cell maintenance and enrichment. In some embodiments, the media and culture conditions comprise one or more growth factors selected from GDNF, FGF2, SDF-1a, CSF-1, FDGF, NGF, and TGF-β, in any combination. In exemplary embodiments, the SSCs are porcine or bovine SSCs.

    A CONTRACEPTIVE VACCINE BASED ON THE SPERM-ASSOCIATED PROTEIN CATSPER
    2.
    发明申请
    A CONTRACEPTIVE VACCINE BASED ON THE SPERM-ASSOCIATED PROTEIN CATSPER 审中-公开

    公开(公告)号：WO2020163853A1

    公开(公告)日：2020-08-13

    申请号：PCT/US2020/017449

    申请日：2020-02-10

    Applicant: RENSSELAER POLYTECHNIC INSTITUTE

    Inventor： BYSTROFF, Christopher ,  CHUNG, Jean-Ju, Lucia

    IPC: A61K39/00 ,  A61P15/16 ,  A61P15/18 ,  A61K35/52 ,  A61K39/395 ,  C07K14/705 ,  C12N7/04

    Abstract: A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

    METHODS OF ESTIMATING GAMETE VARIATION
    4.
    发明申请
    METHODS OF ESTIMATING GAMETE VARIATION 审中-公开

    公开(公告)号：WO2019006342A1

    公开(公告)日：2019-01-03

    申请号：PCT/US2018/040353

    申请日：2018-06-29

    Applicant: INGURAN, LLC

    Inventor： HEUER, Claas

    IPC: A01K67/02 ,  A61K35/52 ,  A61K35/54 ,  C12Q1/68

    Abstract: The invention encompasses a method of estimating a distribution or a standard deviation of breeding values or predicted transmitting abilities of gametes from an individual using population-wide estimates of linkage disequilibrium for one or more genetic markers or genes, so as to more efficiently produce offspring having breeding values or predicted transmitting abilities that equal or exceed a desired breeding value or predicted transmitting ability.

    PREPARATIONS OF DERIVED EXTRACELLULAR VESICLES, ASSAYS, AND METHODS TO MODIFY THERAPEUTIC OUTCOMES USING SUCH PREPARATIONS
    5.
    发明申请
    PREPARATIONS OF DERIVED EXTRACELLULAR VESICLES, ASSAYS, AND METHODS TO MODIFY THERAPEUTIC OUTCOMES USING SUCH PREPARATIONS 审中-公开
    衍生的细胞外细胞的制备，测定和使用这种制剂来修饰治疗成果的方法

    公开(公告)号：WO2015143113A1

    公开(公告)日：2015-09-24

    申请号：PCT/US2015/021394

    申请日：2015-03-19

    Applicant: COHEN, Barb, Ariel

    Inventor： COHEN, Barb, Ariel

    IPC: C12N5/076 ,  A61K35/52

    CPC classification number: A61K35/52 ,  C12N5/061

    Abstract: A method for altering the properties of extracellular vesicles and exosomes for use in diagnosis and therapeutics is disclosed, through their production by primary cell culture under Applicant's specific controlled conditions. A mammalian ejaculate is provided and sperm therefrom are incubated, during which time aliquots are evaluated for production of vesicles engineered to contain the cargo of choice and targeted to the desired application through adjustment of the surface receptor complement. For example, vesicle-borne Fc receptors act as decoys to protect sperm from antibodies in the female tract and vesicles additionally deliver regulatory molecules to modulate tract function, enabling the ability to control release and to engineer sperm-derived vesicle/ exosome structures to be used in diagnosis of infertility and in production of sperm doses of superior performance.

    Abstract translation: 通过根据申请人的特定受控条件通过原代细胞培养来生产用于改变用于诊断和治疗的胞外囊泡和外来体的性质的方法。 提供哺乳动物射精，并从中孵育精子，在此期间评估等分试样的生产，以便通过调整表面受体补体来制备包含所选择的货物并靶向所需应用的囊泡。 例如，囊泡传播的Fc受体作为诱饵来保护精子免于女性道中的抗体，并且囊泡另外传递调节分子以调节功能，使得能够控制释放并且使得精子来源的囊泡/外来体结构被使用 在诊断不孕症和生产精子剂量方面表现优异。

    ANTIBODY FOR SKEWING SEX RATIO AND METHODS OF USE THEREOF
    6.
    发明申请
    ANTIBODY FOR SKEWING SEX RATIO AND METHODS OF USE THEREOF 审中-公开
    用于剔除性别的抗体及其使用方法

    公开(公告)号：WO2015127055A1

    公开(公告)日：2015-08-27

    申请号：PCT/US2015/016589

    申请日：2015-02-19

    Applicant: UNIVERSITY OF TENNESSEE RESEARCH FOUNDATION

    Inventor： EDWARDS, J., Lannett ,  RISPOLI, Louisa, A. ,  SCHRICK, F., Neal

    IPC: A61K35/52

    CPC classification number: G01N33/56966 ,  C07K16/18 ,  C07K16/28 ,  C07K2317/33 ,  C07K2317/34

    Abstract: A purified antibody, or an antigen-binding fragment thereof, is provided that binds selectively to a protein specific to an X-chromosome of a mammalian sperm cell. The sperm cell protein comprises an amino acid sequence set forth in SEQ ID NOs: 4 and 9-16. The antibody or antigen-binding fragment thereof may be derived by immunization of a host by an antigenic peptide composition comprising one or more natural or synthetic antigenic peptide sequences set forth as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NOs: 4 and 9-16. The antibody finds utility in identifying an X-chromosome bearing sperm cell population, and in methods for skewing a sex ratio in mammals.

    Abstract translation: 提供纯化的抗体或其抗原结合片段，其选择性地结合哺乳动物精子细胞的X染色体特异的蛋白质。 精子细胞蛋白质包含SEQ ID NO：4和9-16所示的氨基酸序列。 抗体或其抗原结合片段可以通过由包含SEQ ID NO：1，SEQ ID NO：2，SEQ ID NO：1所示的一种或多种天然或合成抗原肽序列的抗原肽组合物免疫宿主而得到。 3和SEQ ID NO：4和9-16。 该抗体可用于鉴定携带X染色体的精子细胞群体，以及用于歪斜哺乳动物中性别比例的方法。

    COMPOSITION FOR SEPARATING SPERMATOZOA FROM A SEMEN SAMPLE
    10.
    发明申请
    COMPOSITION FOR SEPARATING SPERMATOZOA FROM A SEMEN SAMPLE 审中-公开
    用于从SEMENA样品分离SPERMATOZOA的组合物

    公开(公告)号：WO2009031970A1

    公开(公告)日：2009-03-12

    申请号：PCT/SE2008/050997

    申请日：2008-09-05

    Applicant: MORRELL, Jane ,  RODRIGUEZ-MARTINEZ, Heriberto

    Inventor： MORRELL, Jane ,  RODRIGUEZ-MARTINEZ, Heriberto

    IPC: C12N5/06 ,  A61D19/02 ,  A61K35/52 ,  B01J13/00 ,  C12N3/00

    CPC classification number: C12N1/02 ,  A61K35/52 ,  C12N5/061 ,  C12N5/0612

    Abstract: The present invention relates to the field of veterinary medicine, more specifically to compositions and methods useful for processing animal semen for use in assisted reproduction. The invention relates to a composition for separation of spermatozoa from a semen sample, comprising at least one salt of an alkali metal and/or an alkaline earth metal, EDTA, a zwitterion buffer, silane-coated silica particles and water, said composition having a pH of 7.0- 7.35 and an osmolarity of 300-345 mOsm. Furthermore it relates to a method for preparing spermatozoa from a semen sample from a non-human animal, comprising the step of separating the spermatozoa from other semen constituents by centrifugation through a single layer of a colloid formulation. Preferably, the colloid formulation is the composition according to the invention. The invention also relates to a method for separating a sperm sub-population of interest from a semen sample from a non-human animal, comprising the steps of providing a density gradient comprising at least two layers of the composition of the invention, each layer having a different density;separating the sperm sub-populations in the semen sample by centrifugation through the density gradient; and selecting the sperm sub-population of interest. Finally, the invention relates to spermatozoa prepared with the methods of the invention and the use of such spermatozoa in artificial insemination, in vitro fertilisation or intracytoplasmic sperm injection.

    Abstract translation: 本发明涉及兽医领域，更具体地涉及可用于处理用于辅助繁殖的动物精液的组合物和方法。 本发明涉及一种用于从精液样品中分离精子的组合物，其包含至少一种碱金属和/或碱土金属的盐，EDTA，两性离子缓冲剂，硅烷涂覆的二氧化硅颗粒和水，所述组合物具有 pH 7.0〜7.35，渗透压为300-345mOsm。 此外，它涉及从非人动物的精液样品制备精子的方法，包括通过单层胶体制剂离心分离精子与其他精液成分的步骤。 优选地，胶体制剂是根据本发明的组合物。 本发明还涉及从精液样品与非人类动物分离目标精子亚群的方法，包括提供包含至少两层本发明组合物的密度梯度的步骤，每一层具有 密度不同;通过密度梯度离心分离精液样本中的精子亚群; 并选择感兴趣的精子亚群体。 最后，本发明涉及用本发明的方法制备的精子和这种精子在人工授精，体外受精或胞质内精子注射中的应用。

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