公开(公告)号：WO2011038370A1

公开(公告)日：2011-03-31

申请号：PCT/US2010/050445

申请日：2010-09-27

Applicant: N3D BIOSCIENCES, INC. ,  SOUZA, Glauco, R.

Inventor： SOUZA, Glauco, R.

IPC: B05D5/12 ,  H01F1/04

CPC classification number: C12N5/0062 ,  B03C1/015 ,  B03C1/30 ,  B03C2201/26 ,  B82Y25/00 ,  C12N5/00 ,  C12N2513/00 ,  C12N2529/00 ,  C12N2531/00 ,  C12N2533/10 ,  C12N2533/32 ,  G01N33/54326 ,  G01N33/544 ,  G01R33/1269 ,  H01F1/0045 ,  H01F1/0063 ,  H01F1/083 ,  H01F1/113 ,  H01F1/445 ,  Y10S977/773 ,  Y10S977/89 ,  Y10S977/904 ,  Y10S977/923

Abstract: A material comprising positively and negatively charged nanoparticles, wherein one of said nanoparticles contained a magnetically responsive element, are combined with a support molecule, which is a long natural or synthetic molecule or polymer to make a magnetic nanoparticle assembly. When the magnetic nanoparticle assembly is combined with cells, it will magnetize those cells. The magnetized cells can then be washed to remove the magnetic nanoparticle assembly and the magnetized cells manipulated in a magnetic field.

Abstract translation: 包含正电荷和带负电荷的纳米颗粒的材料，其中所述纳米颗粒中的一个包含磁响应元件，与作为磁性纳米颗粒组件的长天然或合成分子或聚合物的载体分子组合。 当磁性纳米颗粒组件与细胞结合时，它将磁化这些细胞。 然后可以洗涤磁化的细胞以除去磁性纳米颗粒组件，并且磁化的细胞在磁场中操作。

公开(公告)号：WO2010036957A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058473

申请日：2009-09-25

Applicant: WILLIAM MARSH RICE UNIVERSITY ,  SOUZA, Glauco, R. ,  PASQUALINI, Renata ,  ARAP, Wadih ,  KILLIAN, Thomas, Charles ,  RAPHAEL, Robert, M. ,  STARK, Daniel, Joshua

Inventor： SOUZA, Glauco, R. ,  PASQUALINI, Renata ,  ARAP, Wadih ,  KILLIAN, Thomas, Charles ,  RAPHAEL, Robert, M. ,  STARK, Daniel, Joshua

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N13/00 ,  B82Y5/00 ,  C12M35/06 ,  C12N5/0062 ,  C12N2529/00 ,  C12N2533/10

Abstract: Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly.

Abstract translation: 讨论了一般在医学，细胞生物学，纳米技术和细胞培养中有用的系统和方法。 特别地，至少在一些实施例中，讨论了用于细胞和材料的磁性引导和图案化的系统和方法。 这些系统和方法的一些具体应用可以包括将细胞悬浮培养离开表面，制备和操纵悬浮细胞的图案，以及对表面上的细胞进行图案化培养。 具体地说，提出培养细胞的方法。 该方法可以包括提供多个单元，提供磁场，以及悬浮在磁场中的多个单元中的至少一些单元，其中多个单元包括磁性纳米颗粒。 该方法还可以包括将悬浮液维持足以允许细胞生长形成组件的时间。

公开(公告)号：WO2012106089A2

公开(公告)日：2012-08-09

申请号：PCT/US2012/021233

申请日：2012-01-13

Applicant: NANO3D BIOSCIENCES, INC. ,  SOUZA, Glauco, R.

Inventor： SOUZA, Glauco, R.

IPC: C12Q1/02 ,  C12N11/02 ,  G06F17/10

CPC classification number: G01N33/5088 ,  G01N33/5082

Abstract: Cells are grown in 3D culture and topological features obtained by photomicrography are correlated to cell viability and cell cell interactions.

Abstract translation: 细胞在3D培养中生长并且通过显微摄影获得的拓扑特征与细胞活力和细胞细胞相互作用相关。