公开(公告)号：WO2015167710A1

公开(公告)日：2015-11-05

申请号：PCT/US2015/022738

申请日：2015-03-26

Applicant: NATIONAL HEALTH RESEARCH INSTITUTES ,  WANG, Lu-hai

Inventor： CHOW, Yen-hung ,  TSOU, Yueh-liang

IPC: A61K39/12 ,  A61K38/00

CPC classification number: A61K39/125 ,  A61K39/12 ,  A61K2039/5252 ,  A61K2039/5256 ,  A61K2039/5258 ,  A61K2039/542 ,  A61K2039/545 ,  A61K2039/55566 ,  A61K2039/57 ,  C12N2710/10323 ,  C12N2710/10343 ,  C12N2770/32323 ,  C12N2770/32334 ,  C12N2770/32351 ,  C12N2770/32371 ,  Y02A50/466

Abstract: The present invention relates to a recombinant adenoviral vector for generating immunity against enterovirus infection. In one embodiment, the recombinant adenoviral vector of the invention comprises an expression cassette encoding a PI protein and a 3 CD protease of an enterovirus. In another embodiment, the recombinant adenoviral vector of the invention comprises an expression cassette encoding a 3C protease or a 3CD protease of an enterovirus. The present invention also relates to a vaccine composition comprising the recombinant adenoviral vector as described. A method of inducing an immune response in a subject against enterovirus infection using the recombinant adenoviral vector and the vaccine composition is provided. Further provided is a method for producing virus like particles of an enterovirus by expressing the adenoviral vector as described herein in mammalian cells.

Abstract translation: 本发明涉及用于产生针对肠道病毒感染的免疫的重组腺病毒载体。 在一个实施方案中，本发明的重组腺病毒载体包含编码肠道病毒的PI蛋白和3CD蛋白酶的表达盒。 在另一个实施方案中，本发明的重组腺病毒载体包含编码肠病毒的3C蛋白酶或3CD蛋白酶的表达盒。 本发明还涉及包含如上所述的重组腺病毒载体的疫苗组合物。 提供了使用重组腺病毒载体和疫苗组合物诱导受试者免疫应答的方法。 还提供了通过在哺乳动物细胞中表达本文所述的腺病毒载体来产生病毒样肠道病毒颗粒的方法。

公开(公告)号：WO2015179979A1

公开(公告)日：2015-12-03

申请号：PCT/CA2015/050484

申请日：2015-05-28

Applicant: NATIONAL HEALTH RESEARCH INSTITUTES ,  CHONG, Pele Choi-sing

Inventor： CHONG, Pele Choi-sing ,  LIU, Chia-chyi ,  LIN, Hsiao-yu ,  CHOW, Yen-hung

IPC: C07K16/10 ,  A61K39/125 ,  A61K39/295 ,  A61P31/14 ,  C07K14/085 ,  C12N7/00 ,  C12N7/04

CPC classification number: C07K16/1009 ,  A61K39/12 ,  A61K2039/5258 ,  C07K2317/33 ,  C07K2317/76 ,  C12N7/00 ,  C12N2770/32334 ,  C12N2770/32351

Abstract: The present invention relates to viral particles as immunogens against enterovirus infection and a method of producing the same. Specifically, the present invention features that human embryo kidney 293 (HEK 293) cells are used to produce viral particles of Enterovirus A, particularly Coxsackievirus A6 (CVA6) particles or Coxsackievirus A10 (CVA10) particles or both and optionally additional viral particles of other Enterovirus A e.g. Coxsackievirus A16 (CVA16) and/or Enterovirus A71 (EV71). The yield of the viral particles in HEK 293 cells are unexpectedly high and effective to induce an immune response against enterovirus infection, especially CVA6 and CVA10. The present invention also relates to an immunogenic composition against enterovirus infection for human use comprising the viral particles as described herein and a method of preventing enterovirus infection or a disease as caused, particularly Hand-Foot-Mouth diseases (HFMD), by administering the immunogenic composition to a subject in need thereof.

Abstract translation: 本发明涉及作为针对肠道病毒感染的免疫原的病毒颗粒及其制备方法。 具体地，本发明的特征在于使用人胚胎293（HEK 293）细胞产生肠道病毒A的病毒颗粒，特别是柯萨奇病毒A6（CVA6）颗粒或柯萨奇病毒A10（CVA10）颗粒或其他任何另外的病毒颗粒，其它肠道病毒 例如 柯萨奇病毒A16（CVA16）和/或肠病毒A71（EV71）。 HEK 293细胞中病毒颗粒的产量意外高且有效诱导针对肠道病毒感染的免疫应答，特别是CVA6和CVA10。 本发明还涉及针对人类使用的肠道病毒感染的免疫原性组合物，其包含本文所述的病毒颗粒，以及预防肠病毒感染或引起的疾病，特别是手足口病（HFMD）的方法，通过施用免疫原性 组合成有需要的受试者。