公开(公告)号：WO2021242178A1

公开(公告)日：2021-12-02

申请号：PCT/SG2021/050306

申请日：2021-05-31

Applicant: NATIONAL UNIVERSITY OF SINGAPORE

Inventor： YAN, Jie ,  LI, Rong ,  LU, Chen ,  WANG, Yinan ,  ZHAO, Xiaodan ,  ZHOU, Yu ,  LE, Shimin

IPC: G01N33/536 ,  G01N33/68 ,  G01N21/00

Abstract: Disclosed herein is a method of detecting the presence of a target analyte in a sample, as well as a system, an article and a kit thereof, wherein the target analyte comprising biomolecule, bioorganelle, bioparticle, cell and microorganism. In certain embodiment, the method of detecting a target analyte comprising the steps of (I) providing a plurality of probe particles having sensing elements capable of specific interaction with a target analyte bound to a surface coated with another sensing element, (ii) applying mechanical force such as magnetic force to remove non-specifically bound probe particles, and (ill) measuring the amount of specifically bound probe particles or non-specifically bound probe particles through particle density, aggregate size or colour density. In one embodiment, the target analyte is SARS- CoV-2.

公开(公告)号：WO2017151062A1

公开(公告)日：2017-09-08

申请号：PCT/SG2017/050101

申请日：2017-03-03

Applicant: NATIONAL UNIVERSITY OF SINGAPORE

Inventor： YAN, Jie ,  WINARDHI, Ricksen Surya ,  MINGXI, Yao

IPC: G01Q60/24 ,  G01L5/00 ,  G01N21/64 ,  G01N23/20

CPC classification number: G01N27/745 ,  G01N21/648 ,  G01N33/6803 ,  G01N2500/04 ,  G01Q60/42

Abstract: A method of determining a presence of interaction between a substance molecule and a stretched substrate molecule, the method comprising the steps of: providing the stretched substrate molecule, such that a first contact point of the stretched substrate molecule and a second contact point of the stretched substrate molecule are separated at a displacement; contacting the stretched substrate molecule with a solution, wherein the solution comprises the substance molecule or is suspected to comprise the substance molecule; and detecting a change in the displacement between the first contact point and the second contact point of the stretched substrate molecule, wherein the change in the displacement between the first contact point and the second contact point indicates the presence of interaction between the substance molecule and the stretched substrate molecule.

Abstract translation: 一种确定物质分子与拉伸的底物分子之间相互作用的存在的方法，所述方法包括以下步骤：提供拉伸的底物分子，使得拉伸的底物分子的第一接触点 并且拉伸的底物分子的第二接触点在位移处分离; 使拉伸的底物分子与溶液接触，其中该溶液包含物质分子或被怀疑包含物质分子; 以及检测拉伸的基底分子的第一接触点和第二接触点之间的位移的变化，其中第一接触点和第二接触点之间的位移的变化指示物质分子与第二接触点之间的相互作用的存在 拉伸的底物分子。

公开(公告)号：WO2021230821A1

公开(公告)日：2021-11-18

申请号：PCT/SG2021/050264

申请日：2021-05-12

Applicant: NATIONAL UNIVERSITY OF SINGAPORE

Inventor： YAN, Jie ,  LI, Rong ,  LU, Chen ,  ZHAO, Xiaodan ,  ZHOU, Yu ,  LE, Shimin

IPC: G01N33/543 ,  G01N33/569

Abstract: Disclosed herein is a method of detecting the presence or absence of an analyte in a test sample, based on the use of motion resistant particle for example non-magnetic particle coated with first sensing element for the analyte and force driven particle for example superparamagnetic particle coated with a second sensing element for the analyte so as to form a motion resistant particle-analyte-force driven particle conjugate. A viscous medium is added to the mixture and a force such as magnetic force is applied to provide separation of the conjugate for quantification. Disclosed herein are also a sensing kit, a system for detecting the presence or absence of an analyte in a test sample and the use of the kit or the system thereof.