公开(公告)号：WO2017180904A1

公开(公告)日：2017-10-19

申请号：PCT/US2017/027472

申请日：2017-04-13

Applicant: ARIZONA BOARD OF REGENTS on behalf of THE UNIVERSITY OF ARIZONA

Inventor： SCHWARTZ, Jacob ,  MOINPOUR, Mahta

IPC: C07K14/435 ,  C07K14/47 ,  G01N33/68 ,  G01N33/53

CPC classification number: G01N33/533 ,  C07K1/1136 ,  C07K1/13 ,  G01N33/52 ,  G01N33/57484 ,  G01N33/582 ,  G01N33/60 ,  G01N33/68 ,  G01N33/6803 ,  G01N33/6896

Abstract: Methods, systems, and compositions for detecting molecule aggregation, folding, or interactions featuring comparing the amount of labeling of a molecule of interest, such as a protein, in a test sample with an amount of labeling in a control, e.g., a sample wherein the molecule of interest is denatured. If less labeling is present in the test sample as compared to the control sample, the test sample may comprise the molecule of interest in aggregate form, folded form, or interactive form, e.g., interacting with another molecule such as a protein molecule, DNA molecule or RNA molecule. The present invention may be used for detecting or monitoring a disease or condition such as a protein misfolding disease (proteopathy).

Abstract translation: 用于检测分子聚集，折叠或相互作用的方法，系统和组合物，其特征在于将测试样品中感兴趣的分子如蛋白质的标记量与 对照，例如其中感兴趣的分子变性的样品。 如果与对照样品相比，测试样品中存在较少的标记，则测试样品可以包含聚集形式，折叠形式或交互形式的感兴趣分子，例如与另一种分子如蛋白质分子，DNA分子相互作用 或RNA分子。 本发明可用于检测或监测诸如蛋白质错误折叠疾病（蛋白质病）的疾病或病症。

公开(公告)号：WO2017151062A1

公开(公告)日：2017-09-08

申请号：PCT/SG2017/050101

申请日：2017-03-03

Applicant: NATIONAL UNIVERSITY OF SINGAPORE

Inventor： YAN, Jie ,  WINARDHI, Ricksen Surya ,  MINGXI, Yao

IPC: G01Q60/24 ,  G01L5/00 ,  G01N21/64 ,  G01N23/20

CPC classification number: G01N27/745 ,  G01N21/648 ,  G01N33/6803 ,  G01N2500/04 ,  G01Q60/42

Abstract: A method of determining a presence of interaction between a substance molecule and a stretched substrate molecule, the method comprising the steps of: providing the stretched substrate molecule, such that a first contact point of the stretched substrate molecule and a second contact point of the stretched substrate molecule are separated at a displacement; contacting the stretched substrate molecule with a solution, wherein the solution comprises the substance molecule or is suspected to comprise the substance molecule; and detecting a change in the displacement between the first contact point and the second contact point of the stretched substrate molecule, wherein the change in the displacement between the first contact point and the second contact point indicates the presence of interaction between the substance molecule and the stretched substrate molecule.

Abstract translation: 一种确定物质分子与拉伸的底物分子之间相互作用的存在的方法，所述方法包括以下步骤：提供拉伸的底物分子，使得拉伸的底物分子的第一接触点 并且拉伸的底物分子的第二接触点在位移处分离; 使拉伸的底物分子与溶液接触，其中该溶液包含物质分子或被怀疑包含物质分子; 以及检测拉伸的基底分子的第一接触点和第二接触点之间的位移的变化，其中第一接触点和第二接触点之间的位移的变化指示物质分子与第二接触点之间的相互作用的存在 拉伸的底物分子。

公开(公告)号：WO2017139044A1

公开(公告)日：2017-08-17

申请号：PCT/US2017/012089

申请日：2017-01-04

Applicant: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC.

Inventor： FISER, Andras ,  YAP, Enghui

IPC: C40B40/00 ,  C40B40/04 ,  G06F19/00 ,  G06F19/10 ,  G06F19/16

CPC classification number: G06F19/16 ,  G01N33/6803 ,  G01N2500/00

Abstract: A system and a method for identifying a cognate ligand molecules for known proteins.

Abstract translation: 用于鉴定已知蛋白质的同源配体分子的系统和方法。

公开(公告)号：WO2017085086A1

公开(公告)日：2017-05-26

申请号：PCT/EP2016/077785

申请日：2016-11-15

Applicant: CHROMOTEK GMBH ,  NMI NATURWISSENSCHAFTLICHES UND MEDIZINISCHES INSTITUT AN DER UNIVERSITÄT TÜBINGEN

Inventor： ROTHBAUER, Ulrich ,  POETZ, Oliver ,  ROMER, Tina ,  BUCHFELLNER, Andrea ,  YURLOVA, Larisa ,  ZOLGHADR, Kourosh ,  BOGNER, Jaqueline ,  RUF, Benjamin ,  LINKE-WINNEBECK, Christian ,  METTERLEIN, Michael

IPC: C12N15/62 ,  C07K1/22 ,  G01N33/68

CPC classification number: C07K7/06 ,  C07K1/22 ,  C07K7/08 ,  C07K14/47 ,  C07K16/18 ,  C07K2317/34 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/569 ,  C07K2317/92 ,  C07K2319/40 ,  C12N15/62 ,  G01N21/6428 ,  G01N33/58 ,  G01N33/68 ,  G01N33/6803 ,  G01N2021/6439

Abstract: The present invention is concerned with a novel epitope, which can be used as a tag for use in methods for rapid and effective characterization, purification, and subcellular localization of polypeptides of interest, which comprise the tag. The tag is specifically recognized by an epitope specific antibody, which can be used to detect, capture, quantify, and/or purify polypeptides of interest, which are tagged with the epitope of the present invention.

Abstract translation: 本发明涉及一种新型表位，其可以用作用于包含标签的感兴趣多肽的快速和有效表征，纯化和亚细胞定位的方法中的标签。 标签特异性识别表位特异性抗体，其可用于检测，捕获，定量和/或纯化用本发明的表位标记的目的多肽。

公开(公告)号：WO2017062973A3

公开(公告)日：2017-05-26

申请号：PCT/US2016056318

申请日：2016-10-10

Applicant: DICE MOLECULES SV LLC ,  PATTEN PHILLIP ,  HARBURY PEHR ,  SCHMIDT DONALD ,  LINSELL MARTIN

Inventor： PATTEN PHILLIP ,  HARBURY PEHR ,  SCHMIDT DONALD ,  LINSELL MARTIN ,  WLASICHUK KENNETH ,  PRINCE ROBIN ,  CHURCH TIMOTHY J ,  VAN DER LINDEN WOUTER

IPC: C12M1/34 ,  G01N30/00

CPC classification number: G01N33/92 ,  B01D15/325 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6876 ,  C12Q2600/136 ,  G01N30/02 ,  G01N33/15 ,  G01N33/6803 ,  G01N2030/027 ,  C12Q2563/179 ,  C12Q2565/125 ,  C12Q2565/514 ,  C12Q2565/119 ,  C12Q2565/137

Abstract: Disclosed herein are methods of estimating LogD of compounds comprising a ligand operatively linked to a recognition element. The methods generally involve contacting the compounds with a matrix, measuring the interaction of the compounds with the matrix and relating the interaction of the compounds with the matrix to LogD of the compounds. Also, described herein, are novel compounds which include a ligand operatively linked to a recognition element and linkers that connect the ligand to the recognition element.

Abstract translation: 本文公开了估计包含可操作地连接至识别元件的配体的化合物的LogD的方法。 该方法通常包括使化合物与基质接触，测量化合物与基质的相互作用并将化合物与基质的相互作用与化合物的LogD相关联。 此外，本文所述的新化合物包括与识别元件有效连接的配体和将配体连接至识别元件的接头。

公开(公告)号：WO2017075552A1

公开(公告)日：2017-05-04

申请号：PCT/US2016/059609

申请日：2016-10-29

Applicant: 20/20 GENESYSTEMS INC.

Inventor： RAIT, Vladimir

IPC: C07H21/00 ,  C12N3/00 ,  C12Q1/68

CPC classification number: G01N33/6803 ,  C12Q1/04 ,  G01N33/52

Abstract: Methods, kits and reagents are provided for increasing the sensitivity of detecting the presence or absence of endospores by increasing the available protein for detection. The methods are fast and amendable to testing in a non-laboratory setting and use a protein detection reagent and solid microparticles.

Abstract translation: 通过增加用于检测的可用蛋白来提供方法，试剂盒和试剂以提高检测内生孢子存在或不存在的灵敏度。 该方法速度快，可在非实验室环境下进行检测，并使用蛋白质检测试剂和固体微粒。

公开(公告)号：WO2017062973A2

公开(公告)日：2017-04-13

申请号：PCT/US2016/056318

申请日：2016-10-10

Applicant: DICE MOLECULES SV, LLC ,  PATTEN, Phillip ,  HARBURY, Pehr ,  SCHMIDT, Donald ,  LINSELL, Martin

Inventor： PATTEN, Phillip ,  HARBURY, Pehr ,  SCHMIDT, Donald ,  LINSELL, Martin ,  WLASICHUK, Kenneth ,  PRINCE, Robin ,  CHURCH, Timothy, J. ,  VAN DER LINDEN, Wouter

IPC: G01N33/92 ,  C12Q1/68

CPC classification number: G01N33/92 ,  B01D15/325 ,  C07H21/04 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6876 ,  C12Q2600/136 ,  G01N30/02 ,  G01N33/15 ,  G01N33/6803 ,  G01N2030/027 ,  C12Q2563/179 ,  C12Q2565/125 ,  C12Q2565/514 ,  C12Q2565/119 ,  C12Q2565/137

Abstract: Disclosed herein are methods of estimating LogD of compounds comprising a ligand operatively linked to a recognition element. The methods generally involve contacting the compounds with a matrix, measuring the interaction of the compounds with the matrix and relating the interaction of the compounds with the matrix to LogD of the compounds. Also, described herein, are novel compounds which include a ligand operatively linked to a recognition element and linkers that connect the ligand to the recognition element.

Abstract translation: 本文公开了估计包含可操作地连接到识别元件的配体的化合物的LogD的方法。 方法通常包括使化合物与基质接触，测量化合物与基质的相互作用并将化合物与基质的相互作用与化合物的LogD相关联。 此外，本文描述的是包括可操作地连接到识别元件的配体和将配体连接到识别元件的接头的新化合物。

公开(公告)号：WO2017040323A2

公开(公告)日：2017-03-09

申请号：PCT/US2016/049083

申请日：2016-08-26

Applicant: DANA-FARBER CANCER INSTITUTE, INC.

Inventor： WALENSKY, Loren D. ,  BIRD, Gregory H.

IPC: C07K7/64 ,  G01N30/72

CPC classification number: G01N30/72 ,  C07K7/08 ,  C07K14/00 ,  G01N33/6803 ,  G01N2030/8831

Abstract: Provided herein is a platform technology for designing stabilized peptides that covalently bind their target protein and thereby inhibit the activity of the target protein. Also provided are exemplary stabilized peptides that can be used for covalent modification of their target proteins.

Abstract translation: 本文提供了用于设计与其靶蛋白共价结合从而抑制靶蛋白活性的稳定化肽的平台技术。 还提供了可用于共价修饰其靶蛋白的示例性稳定化肽。

公开(公告)号：WO2016173542A1

公开(公告)日：2016-11-03

申请号：PCT/CN2016/080679

申请日：2016-04-29

Applicant: WINNOZ TECHNOLOGY, INC

Inventor： HSIUNG, Le-Chang ,  TAI, Chun-Yen ,  CHEN, Yu-Lin ,  LIN, Fang-Yu ,  OU, Chuan Whatt Eric

IPC: G01N27/414

CPC classification number: B01L3/502753 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/087 ,  B01L2300/0883 ,  B01L2400/0406 ,  B01L2400/043 ,  B01L2400/088 ,  C12Q1/6806 ,  G01N33/6803 ,  G01N33/721

Abstract: A system (300) for detecting a target (324) and a method for detecting a target (324) are provided. The system (300) includes a field effect transistor (310), having a gate (312), a source (316), and a drain (314); a potentiostat (330), having a working electrode (332), a counter electrode (334), and a reference electrode (336); wherein the working electrode (332) is coupled with a detection region (320), and the counter electrode (334) is coupled with the gate (312); wherein the detection region (320), the gate (312), and the reference electrode (336) are arranged in an ion fluid (302); wherein the potentiostat (330) is configured to generate redox in the ion fluid (302) by an electrochemical method to detect the target (324).

Abstract translation: 提供了一种用于检测目标（324）的系统（300）和用于检测目标（324）的方法。 系统（300）包括具有栅极（312），源极（316）和漏极（314）的场效应晶体管（310）。 具有工作电极（332），对电极（334）和参考电极（336）的恒电位仪（330）; 其中所述工作电极（332）与检测区域（320）耦合，并且所述对置电极（334）与所述栅极（312）耦合; 其中所述检测区域（320），所述栅极（312）和所述参考电极（336）被布置在离子流体（302）中; 其中所述恒电位仪（330）被配置为通过电化学方法在所述离子流体（302）中产生氧化还原以检测所述靶（324）。

公开(公告)号：WO2016153187A1

公开(公告)日：2016-09-29

申请号：PCT/KR2016/002134

申请日：2016-03-03

Applicant: 포항공과대학교 산학협력단

Inventor： 박재성 ,  신현우 ,  김종민

IPC: C12Q1/24 ,  C12N5/00 ,  C12N13/00 ,  G01N33/535 ,  C12Q1/68 ,  G01N33/68 ,  C12M1/26

CPC classification number: C12M1/264 ,  C12M1/26 ,  C12M1/42 ,  C12N5/00 ,  C12N5/0081 ,  C12N13/00 ,  C12Q1/24 ,  C12Q1/68 ,  C12Q1/686 ,  G01N33/535 ,  G01N33/6803

Abstract: 본 발명은 단백질과 혼합되어 오염된 세포 밖 소포체를 수용액 이상계를 이용한 다단계 정제방법을 통하여 짧은 시간 안에 고순도로 많은 양을 분리 및 정제할 수 있는, 수용액 이상계를 이용한 세포 밖 소포체의 다단계 정제방법 및 장치에 관한 것으로, 95 % 이상의 단백질을 제거할 수 있으며, 고순도의 세포 밖 소포체를 획득할 수 있어 종래의 기술에 비하여 매우 높은 효율을 갖는다. 특히 초고속 원심분리 장치나 항체와 같은, 고가의 장비나 재료가 필요 없기에, 비용이 적게 들어 경제적이므로, 경쟁력을 높일 수 있는 방법이다. 또한, 이렇게 분리 및 정제된 세포 밖 소포체는 RT-PCR이나 western blot과 같은 분석 방법에 이용할 수 있어, 이를 이용한 연구 분야 및 질병 진단에도 활용할 수 있다.

Abstract translation: 本发明涉及使用水溶液两相体系的细胞外囊泡的多级纯化方法和装置，其可以在短时间内以高纯度分离和纯化大量由于与蛋白质混合而污染的细胞外小泡 时间通过使用水溶液两相体系的多级纯化方法，其中可以除去95％或更多的蛋白质并获得高纯度的细胞外囊泡，从而与现有技术相比具有非常高的效率。 特别地，该方法成本较低并且是经济的，因此能够提高竞争力，因为不需要昂贵的设备或诸如高速离心分离器或抗体的材料。 此外，分离和纯化的细胞外囊泡可用于分析方法如RT-PCR或western印迹，从而用于使用其的疾病的研究和诊断领域。