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    BACILLUS LICHENIFORMIS HOST CELL WITH DELETED LANTIBIOTIC GENE(S)
    1.
    发明申请
    BACILLUS LICHENIFORMIS HOST CELL WITH DELETED LANTIBIOTIC GENE(S) 审中-公开
    BACILLUS LICHENIFORMIS HOST CELL WITH DELETED LANTIBIOTIC GENE（S）

    公开(公告)号：WO2016180928A1

    公开(公告)日：2016-11-17

    申请号：PCT/EP2016/060713

    申请日：2016-05-12

    Applicant: NOVOZYMES A/S

    Inventor： KOEBMANN, Brian ,  RASMUSSEN, Michael, Dolberg

    IPC: C12N1/20 ,  C12P21/00 ,  C12N15/75

    CPC classification number: C12N15/75 ,  C07K14/32 ,  C12P21/02

    Abstract: The present invention relates to Bacillus licheniformis host cells producing heterologous polypeptide of interest, wherein at least one gene in the Ian gene cluster inactivated and methods for producing the polypeptide of interest by cultivating said cells.

    Abstract translation: 本发明涉及产生感兴趣的异源多肽的地衣芽孢杆菌宿主细胞，其中Ian基因簇中的至少一个基因被灭活，并且通过培养所述细胞来产生感兴趣的多肽的方法。

    DIRECT TRANSFER OF POLYNUCLEOTIDES BETWEEN GENOMES
    4.
    发明申请
    DIRECT TRANSFER OF POLYNUCLEOTIDES BETWEEN GENOMES 审中-公开
    基因组间多核苷酸的直接转移

    公开(公告)号：WO2015004013A1

    公开(公告)日：2015-01-15

    申请号：PCT/EP2014/064289

    申请日：2014-07-04

    Applicant: NOVOZYMES A/S

    Inventor： RASMUSSEN, Michael, Dolberg ,  POULSEN, Gitte, Bak

    IPC: C12N15/10

    CPC classification number: C12N15/902 ,  C12N15/1082 ,  C12N15/75

    Abstract: The present invention provides methods for directly transferring a recombinant polynucleotide of interest from the chromosome of a prokaryotic donor cell to the chromosome of a prokaryotic recipient cell of another species, wherein the polynucleotide of interest comprises at least one coding sequence of interest, preferably one gene of interest and a selectable marker, wherein said polynucleotide of interest in the chromosome of the donor cell is flanked on each side by a polynucleotide region derived from the chromosome of the recipient cell, and wherein the flanking polynucleotide regions are of sufficient size and sequence identity to allow double homologous recombination with the corresponding regions in the chromosome of the recipient cell.

    Abstract translation: 本发明提供了将目的重组多核苷酸从原核供体细胞的染色体直接转移到另一物种的原核细胞受体细胞的染色体的方法，其中所述目的多核苷酸包含至少一个感兴趣的编码序列，优选一个基因 感兴趣的靶标和选择性标记，其中所述供体细胞染色体中所述感兴趣的多核苷酸在每侧由位于受体细胞的染色体的多核苷酸区域侧接，并且其中所述侧翼多核苷酸区域具有足够的大小和序列同一性 以允许与受体细胞的染色体中的相应区域的双重同源重组。

    A CELL WITH IMPROVED SECRETION MEDIATED BY MRGA PROTEIN OR HOMOLOGUE
    5.
    发明申请
    A CELL WITH IMPROVED SECRETION MEDIATED BY MRGA PROTEIN OR HOMOLOGUE 审中-公开
    一种细胞与MRGA蛋白或HOMOLOGY介导的改良分泌

    公开(公告)号：WO2005056799A1

    公开(公告)日：2005-06-23

    申请号：PCT/DK2004/000859

    申请日：2004-12-10

    Applicant: NOVOZYMES A/S ,  NIELSEN, Allan, Kent ,  RASMUSSEN, Michael, Dolberg

    Inventor： NIELSEN, Allan, Kent ,  RASMUSSEN, Michael, Dolberg

    IPC: C12N15/31

    CPC classification number: C12N15/75 ,  C07K14/32 ,  C12P21/02

    Abstract: A progeny cell derived from a parent cell, wherein the progeny cell comprises at least one gene encoding MrgA protein or a functional homologue thereof and/or a DNA segment operably linked with the encoding gene, wherein said gene and/or DNA segment is manipulated with respect to the parent cell; the progeny cell comprises two or more copies of a gene encoding MrgA protein or a functional homologue thereof; or the progeny cell is mutated with respect to the parent cell; whereby the progeny cell produces greater amounts of MrgA protein or a functional homologue thereof than the parent cell.

    Abstract translation: 衍生自亲本细胞的子代细胞，其中所述后代细胞包含编码MrgA蛋白或其功能同系物的至少一个基因和/或与所述编码基因可操作地连接的DNA片段，其中所述基因和/或DNA片段用 尊重父母细胞; 子代细胞包含编码MrgA蛋白或其功能同系物的基因的两个或更多拷贝; 或后代细胞相对于亲本细胞突变; 由此后代细胞比亲本细胞产生更大量的MrgA蛋白或其功能同系物。

    METHOD FOR STABLE GENE-AMPLIFICATION IN A BACTERIAL HOST CELL
    8.
    发明申请
    METHOD FOR STABLE GENE-AMPLIFICATION IN A BACTERIAL HOST CELL 审中-公开
    用于在细菌宿主细胞中稳定的基因扩增的方法

    公开(公告)号：WO2005042750A1

    公开(公告)日：2005-05-12

    申请号：PCT/DK2004/000750

    申请日：2004-10-29

    Applicant: NOVOZYMES A/S ,  OLSEN, Carsten ,  RASMUSSEN, Michael, Dolberg

    Inventor： OLSEN, Carsten ,  RASMUSSEN, Michael, Dolberg

    IPC: C12N15/69

    CPC classification number: C12N15/75 ,  C12N9/1007 ,  C12N9/2471 ,  C12P21/00 ,  C12Y201/01014 ,  C12Y302/01023

    Abstract: A bacterial host cell comprising at least two copies of an amplification unit in its genome, said amplification unit comprising: i) at least one copy of a gene of interest, and ii) an expressible conditionally essential gene, wherein the conditionally essential gene is either promoterless or transcribed from a heterologous promoter having an activity substantially lower than the endogenous promoter of said conditionally essential gene, and wherein the conditionally essential gene if not functional would render the cell auxotrophic for at least one specific substance or unable to utilize one or more specific sole carbon source; methods for producing a protein using the cell of the invention, and methods for constructing the cell of the invention.

    Abstract translation: 一种细菌宿主细胞，其在其基因组中包含至少两个拷贝的扩增单元，所述扩增单元包含：i）目的基因的至少一个拷贝，和ii）可表达的条件必需基因，其中条件必需基因是 来自具有显着低于所述条件必需基因的内源启动子的活性的异源启动子的无启动子或转录，并且其中所述条件必需基因如果不是功能性的将使细胞对于至少一种特定物质具有营养缺陷型或不能利用一种或多种特异性 唯一碳源 使用本发明的细胞产生蛋白质的方法以及构建本发明的细胞的方法。

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