公开(公告)号：WO2021146443A1

公开(公告)日：2021-07-22

申请号：PCT/US2021/013465

申请日：2021-01-14

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

Inventor： BERMAN, Russell ,  BENITEZ-MARZAN, Jaime Juan ,  POPOVICH, Natasha ,  RULISON, Aaron ,  SAXENA, Ravi

IPC: G01N21/03 ,  G01N21/64 ,  G01N21/77 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2201/0873

Abstract: Provided herein are cartridges, packaged devices, and systems for improved nucleic acid sequencing. The cartridges, devices, and systems include a highly multiplexed optical chip comprising a plurality of nanoscale reaction regions that is configured to perform and report nucleic acid sequencing reactions. The chips are, in some embodiments, packaged for use in analytical nucleic acid sequencing reactions. The chips may be attached to a printed circuit board, may be surrounded by a protective enclosure, may include a flow cell, and may include optical features to minimize or block photobleaching of the sequencing reagents and background fluorescent signals. Also provided are analytical systems for nucleic acid sequencing that comprise the disclosed cartridges and packaged devices. The systems comprise an analytical instrument with electronic, optical, mechanical, fluidic, and/or thermal connectors designed to interact with the corresponding connectors on an associated cartridge or packaged device in a highly precise but reversible manner.

公开(公告)号：WO2019090192A1

公开(公告)日：2019-05-09

申请号：PCT/US2018/059112

申请日：2018-11-03

Applicant: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.

Inventor： KURITSYN, Alexey ,  MCDONALD, Mark ,  RULISON, Aaron ,  BERMAN, Russell

IPC: G02B6/124 ,  G01N21/17 ,  G01N21/63 ,  G01N21/64 ,  G01N21/77 ,  G02B6/34

CPC classification number: G02B6/12016 ,  G01N21/17 ,  G01N21/63 ,  G01N21/64 ,  G01N21/648 ,  G01N21/77 ,  G01N21/7703 ,  G02B6/122 ,  G02B6/124 ,  G02B6/34 ,  G02B6/422 ,  G02B6/4224 ,  G02B27/0955 ,  G02B27/0972 ,  G02B27/0977 ,  G02B2006/12147 ,  G02B2006/1215

Abstract: Provided herein are systems, devices, and methods for improved optical waveguide transmission and alignment in an analytical system. Waveguides in optical analytical systems can exhibit variable and increasing back reflection of single-wavelength illumination over time, thus limiting their effectiveness and reliability. The systems are also subject to optical interference under conditions that have been used to overcome the back reflection. Novel systems and approaches using broadband illumination light with multiple longitudinal modes have been developed to improve optical transmission and analysis in these systems. Novel systems and approaches for the alignment of a target waveguide device and an optical source are also disclosed.